Nile tilapia CXCR4, the receptor of chemokine CXCL12, is involved in host defense against bacterial infection and chemotactic activity

Gao, Along; Li, Lan; Yan, Fangfang; Yang, Lei; Chen, Jianlin; Wu, Liting; Ye, Jianmin

doi:10.1016/j.dci.2020.103836

Cited by 12 publications

(12 citation statements)

References 80 publications

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“…The expression of the pro-inflammatory and immune related genes (IL-8, CC-chemokine, and C-X-C chemokine) is associated with the fish's response to cope with the stress features. The response includes several functions: promoting adherence of neutrophils to endothelial cells, neutrophil chemo-attractant ability, histamine release, and subsequent migration toward inflammatory sites [63]. Likewise, the expression of TLR7 is related to the antistress response of fish to relieve inflammation impacts [64].…”

Section: Discussionmentioning

confidence: 99%

The Effect of Stocking Density and Carbon Sources on the Oxidative Status, and Nonspecific Immunity of Nile tilapia (Oreochromis niloticus) Reared under Biofloc Conditions

Shourbela

Khatab

Hassan

et al. 2021

Animals

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The present study investigated the effect of stocking density and dietary carbon sources on the water quality, oxidative status, and immune-related genes of Nile tilapia (Oreochromis niloticus) reared under biofloc conditions (BFT). Eight groups were established at two levels of stocking densities (140 fish per m3: low stocking density, LSD) and (280 fish per m3: high stocking density, HSD) (5.15 ± 1.12 g) and kept in eight biofloc units containing water without carbon sources (control groups) or with glycerol, molasses, or starch. Red blood cells count, hemoglobin, and hematocrit values were reduced in fish stocked in control groups at LSD and HSD than biofloc groups. Control fish groups reared at both LSD and HSD have the highest significant (p < 0.05) white blood cells number than other fish groups. Meanwhile, fish groups that received glycerol, molasses, and starch maintained in both LSD and HSD presented a higher significant (p < 0.05) monocyte % than in the control group reared at both LSD and HSD. The fish group reared in biofloc conditions (BFT) using starch carbon source and reared at the HSD presented a significantly higher (p < 0.05) increase in total serum protein and albumin levels as well as globulin value than the control fish group reared at both LSD and HSD. The highest glucose and cortisol levels were showed in the control fish group reared at both LSD and HSD. Fish maintained in glycerol-based biofloc at LSD attained the highest (p < 0.05) serum superoxide dismutase (SOD), glutathione reductase (GR), and catalase than other experimental groups. Regarding the nonspecific immune status, significantly increased expression of CC-chemokines, CXC-chemokines, TLR7 and IL-8 genes was found in molasses based biofloc groups. The data of the present study revealed that using molasses promotes health status of Nile tilapia cultured in a biofloc system.

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Section: Discussionmentioning

confidence: 99%

The Effect of Stocking Density and Carbon Sources on the Oxidative Status, and Nonspecific Immunity of Nile tilapia (Oreochromis niloticus) Reared under Biofloc Conditions

Shourbela

Khatab

Hassan

et al. 2021

Animals

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“…Furthermore, chemokines play a crucial role in certain stages of tumor development [ 3 , 4 ]. Of these different chemokines, CXCL12 possesses a strong chemotactic ability to cells expressing high levels of CXCR4 [ 5 ]. In addition, the interaction between CXCL12 and its receptor CXCR4 has generated widespread interest with regard to tumor progression [ 6 ].…”

Section: Introductionmentioning

confidence: 99%

Recent Advances in CXCL12/CXCR4 Antagonists and Nano-Based Drug Delivery Systems for Cancer Therapy

Zhao

Liu

et al. 2022

Pharmaceutics

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Chemokines can induce chemotactic cell migration by interacting with G protein-coupled receptors to play a significant regulatory role in the development of cancer. CXC chemokine-12 (CXCL12) can specifically bind to CXC chemokine receptor 4 (CXCR4) and is closely associated with the progression of cancer via multiple signaling pathways. Over recent years, many CXCR4 antagonists have been tested in clinical trials; however, Plerixafor (AMD3100) is the only drug that has been approved for marketing thus far. In this review, we first summarize the mechanisms that mediate the physiological effects of the CXCL12/CXCR4 axis. Then, we describe the use of CXCL12/CXCR4 antagonists. Finally, we discuss the use of nano-based drug delivery systems that exert action on the CXCL12/CXCR4 biological axis.

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“…The fish were immunized with 100 μL (1 × 10 6 CFU/fish, 1 × 10 7 CFU/mL concentration) live bacteria liquid such as S. agalactiae (ZQ1901) or A. hydrophila (BYK00810) dissolved in PBS (10 mM phosphate, 150 mM NaCl, pH 7.4), and grouped in stimulation groups, respectively [ 35 ]. As for the control group, PBS of equal volume was immunized [ 25 , 36 , 37 , 38 ].…”

Section: Methodsmentioning

confidence: 99%

Functional Characterization of Serum Amyloid P Component (SAP) in Host Defense against Bacterial Infection in a Primary Vertebrate

Bai

Yin

et al. 2022

IJMS

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Serum amyloid P component (SAP), an ancient short pentraxin of the pentraxin family, plays an essential role in resistance to bacterial infection. In this study, the expression and functional characterization of SAP (OnSAP) in Nile tilapia (Oreochromis niloticus), a primary vertebrate, are investigated. The open reading frame of OnSAP is 645 bp of a nucleotide sequence encoding a polypeptide of 214 amino acids. As a calcium-binding protein, the structure and relative motif of OnSAP is highly similar to those of humans, containing amino acid residues Asn, Glu, Gln and Asp. In healthy fish, OnSAP mRNA is extensively distributed in all eleven tissues examined, with the highest level in spleen. The mRNA expression of OnSAP was significantly up-regulated after being challenged with gram-positive bacterium Streptococcus agalactiae and gram-negative bacterium Aeromonas hydrophila in vivo. In addition, recombinant OnSAP ((r)OnSAP) protein had capacities of binding S. agalactiae or A. hydrophila in the presence of Ca2+. Further, (r)OnSAP helped monocytes/macrophages to efficiently phagocytize bacteria. Moreover, the (r)OnSAP was able to enhance the complement-mediated lysis of the chicken red blood cells. Collectively, the evidence of SAP in tilapia, based on the results including its evolutionary conserved protein structure, bacterial binding and agglutination, opsonophagocytosis of macrophage and hemolysis enhancement, enriches a better understanding of the biological functions of the pentraxin family.

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Nile tilapia CXCR4, the receptor of chemokine CXCL12, is involved in host defense against bacterial infection and chemotactic activity

Cited by 12 publications

References 80 publications

The Effect of Stocking Density and Carbon Sources on the Oxidative Status, and Nonspecific Immunity of Nile tilapia (Oreochromis niloticus) Reared under Biofloc Conditions

The Effect of Stocking Density and Carbon Sources on the Oxidative Status, and Nonspecific Immunity of Nile tilapia (Oreochromis niloticus) Reared under Biofloc Conditions

Recent Advances in CXCL12/CXCR4 Antagonists and Nano-Based Drug Delivery Systems for Cancer Therapy

Functional Characterization of Serum Amyloid P Component (SAP) in Host Defense against Bacterial Infection in a Primary Vertebrate

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