Nine absorbed components pharmacokinetic of raw and processed Moutan Cortex in normal and blood‐heat and hemorrhage syndrome model rats

Abstract

Raw Moutan Cortex (RMC) and Processed Moutan Cortex (PMC) have a long history of use in China and other Asian countries. In this study, a rapid and accurate ultra‐high‐pressure liquid chromatography coupled with diode array detector (UHPLC–DAD) method was developed and validated for the simultaneous determination of nine absorbed compounds of RMC/PMC. After extraction by protein precipitation with methanol from plasma, the analytes were separated on an Acquity UPLC® BEH Shield RP18 column (2.1 × 100 mm, 1.7 μm, Waters, USA). Acetonitrile (A) and 0.1% (v/v) formic acid in water (B) were selected as the mobile phase to perform gradient elution. The linearity of nine analytes was >0.9915. The intra‐ and inter‐assay precision (RSD) values were within 11.18%, and accuracy ranged from 91.32 to 101.29%. Suitable stability, matrix effect and extraction recoveries were also obtained. The validated method was applied to compare the pharmacokinetics of RMC and PMC in Blood‐Heat and Hemorrhage Syndrome Model and normal rats. The results revealed that processing and the pathological state could influence the pharmacokinetic characteristics of compounds in RMC/PMC. The study willbe useful for further studies on pharmacokinetics and clinical application of raw and processed Moutan Cortex.

The influence of essential oils from ZhaLi NuSi Prescription on the pharmacokinetics of its non‐volatile components in normal rats

“…However, the concentration of the second peak did not increase significantly in group NEO + EO, so that the t max value was significantly longer in group NEO. A similar phenomenon had been reported before (Ye et al, 2020), where the concentration of the second peak was significantly higher. The conversion between components may be the reason for the second peak after the oral administration.…”

Integrating pharmacokinetics and network analysis to investigate the mechanism of Moutan Cortex in blood-heat and blood stasis syndrome

“…However, Cortex Moutan Carbon, the Processed product of Moutan Cortex (PMC), had the opposite effect of cooling blood and hemostatic. Our last article research found that PMC may promote the absorption of 5-HMF and inhibit oxypaeoniflorin, quercetin and 3, 8-Dihydroxy-2- methylchromone absorption in Blood-Heat and Hemorrhage Syndrome Model rat (BHH) as compared with the normal rats [ 37 ]. The absorption of three components (3, 8-dihydroxy-2-methylchromone, benzoic acid, quercetin) was promoted and 5-HMF was inhibited in BHS-RMC.…”

“…The chromatography experiment was carried out using UPLC system (Thermo Fisher Scientific, USA). Chromatographic conditions were according to the paper published by our research group [ 37 ]. Column: Acquity UPLC ® BEH Shield RP 18 Column (2.1 mm × 100 mm, 1.7 μm, Waters, USA).…”

“…The twice filtrates were collected and combined, and vacuum concentrated into an alcohol-free extractum. Last, the extractum was dissolved in water (equal to 6 g/mL of RMC) and stored in seal at 4 °C [36][37][38].…”

Simultaneous Quantification of Four Ginsenosides in Rat Plasma and Its Application to a Comparative Pharmacokinetic Study in Normal and Depression Rats Using UHPLC-MS/MS

“…UHPLC-MS/MS has been widely focused on the preclinical analytical application research and pharmacokinetics during the last decade [ 26 ]. Small particle sizes in columns in UHPLC systems play a crucial role in the process of separation, which can not only increase separation efficiency and sensitivities but also reduce the chromatographic run time [ 27 ].…”