Nineteen polymorphic microsatellite markers from two African Vanilla species: across-species transferability and diversity in a wild population of V. humblotii from Mayotte

Gigant, Rodolphe; Brugel, A.; Bruyn, Alexandre De; Risterucci, Ange Marie; Guiot, V.; Viscardi, G. P.; Humeau, Laurence; Grisoni, Michel; Besse, Pascale

doi:10.1007/s12686-011-9489-1

Cited by 10 publications

(11 citation statements)

References 11 publications

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“…Here we demonstrated that none of them were transferable to Vanilla subgenus Vanilla. On the other hand, the 19 microsatellite markers developed from the Vanilla subgenus Xanata section Tethya African species V. humblotii and V. roscheri were highly transferable to other species from the same section (18 markers in mean were transferable) as well as to various American species from section Xanata (with however a slightly lower mean of 15.7 transferable loci) [21]. We showed that only six of them were transferable to Vanilla subgenus Vanilla.…”

Section: Discussionmentioning

confidence: 68%

“…Within our current efforts to determine the reproductive biology and genetic diversity in vanilla CWR, which led us so far to study V. roscheri Rchb. f. in South Africa [20] and V. humblotii in Mayotte [13,21], we focused on wild populations of V. mexicana occurring in the island of Guadeloupe (French west indies) to unravel its mating system. The vast majority of Vanilla species displays a mixed reproductive mode [1,4] with both asexual and sexual reproduction.…”

Section: Introductionmentioning

confidence: 99%

“…As no specific markers were available for V. mexicana, we used microsatellite markers previously developed in other Vanilla species: the cultivated species V. planifolia (an American species from the genus Vanilla subgenus Xanata section Xanata)[2], V. humblotii and V. roscheri (African species from the genus Vanilla subgenus Xanata section Tethya)[21]. We performed genetic analyses and conducted reproductive biology experiments on V. mexicana wild populations from the island of Guadeloupe (French West Indies) to unravel its mating system.…”

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confidence: 99%

“…and 19 microsatellite markers isolated from V. humblotii and V. roscheri[21] were tested in V. mexicana. Only six markers (from V. humblotii and V. roscheri) were transferable to V. mexicana, giving readable and repeatable amplifications and were used for subsequent PCR amplifications.…”

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confidence: 99%

“…Only six markers (from V. humblotii and V. roscheri) were transferable to V. mexicana, giving readable and repeatable amplifications and were used for subsequent PCR amplifications. These were HU03, HU04, HU06, HU07, HU09, and RO05 using appropriate fluorochrome dyes (see[21] for primer sequences and dyes). PCR volume was 15 µl including 7.5 µl of 2X Qiagen multiplex PCR Master Mix buffer (Qiagen, Hilden/Germany), 0.2 µl of each primer at 20 µM, 5.1 µl HPLC water, and 2 µl DNA (10 ng µl -1 ).…”

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confidence: 99%

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Microsatellite Markers Confirm Self‐Pollination and Autogamy in Wild Populations of Vanilla mexicana Mill. (syn. V. inodora) (Orchidaceae) in the Island of Guadeloupe

Gigant¹,

Rakotomanga²,

Goulié³

et al. 2016

Microsatellite Markers

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The study aimed at evaluating the mating system of Vanilla mexicana (Orchidaceae) in natural populations in the island of Guadeloupe. A total of 132 V. mexicana samples were collected from 12 sites in Guadeloupe (Basse-Terre). Five other samples coming from Martinique and Mexico completed our analyses. Reproductive biology experiments excluding pollinators with bagged flowers revealed 53.9% fruit set, a value identical to the natural fruit set measured in the populations. These results suggested that V. mexicana, unlike most Vanilla species, was reproducing by self-pollination and autogamy. Due to lack of specific DNA markers for V. mexicana, microsatellite markers, previously developed in other Vanilla species, were used for the genetic analyses. Only 6 out of the 33 markers tested were transferable and polymorphic in V. mexicana. A panel of 51 V. mexicana samples genotyped with 3 polymorphic loci was finally retained for Guadeloupe population genetic analyses. A heterozygote deficiency was detected, and the selfing rate was estimated to 74%. These results confirmed the reproductive biology results as self-pollination and autogamy were the most likely explanation for this deficit. Results were compared to those from allogamous wild Vanilla species and discussed in the light of suggested existence of a pollinator for V. mexicana in other areas (Mexico).

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Section: Discussionmentioning

confidence: 68%

Section: Introductionmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Microsatellite Markers Confirm Self‐Pollination and Autogamy in Wild Populations of Vanilla mexicana Mill. (syn. V. inodora) (Orchidaceae) in the Island of Guadeloupe

Gigant¹,

Rakotomanga²,

Goulié³

et al. 2016

Microsatellite Markers

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Drivers of population divergence and species differentiation in a recent group of indigenous orchids (Vanilla spp.) in Madagascar

Andriamihaja

Ramarosandratana

Grisoni

et al. 2021

Ecology and Evolution

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Active sexual reproduction but no sign of genetic diversity in range-edge populations of Vanilla roscheri Rchb. f. (Orchidaceae) in South Africa

et al. 2014

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International audienceIn South Africa, the wild leafless Vanilla roscheri Rchb. f. (Orchidaceae) is distributed only on the banks of the Lake Sibaya in KwaZulu-Natal Coastal Forest in the iSimangaliso Wetland Park. It is the southernmost vanilloid orchid on the African continent and a species of high conservation priority with little understanding of its reproductive biology and levels of genetic diversity. Mating systems and pollination ecology of the species were assessed by in situ experiments, in addition to genotyping plants at 16 microsatellite markers. Allogamous but self-compatible, V. roscheri depends on pollinators to achieve sexual reproduction. Its natural fruit set (26.3 %) is the highest reported for a non-spontaneously self-pollinating Vanilla species. It was associated with numerous flower visitors including two female allodapine bees (Allodapula variegata and Allodape rufogastra, Xylocopinae), captured with pollinia stored on the hind legs, and one female anthophorine bee. On the other hand, we report an absence of genetic diversity and homozygosity of the South-African populations of V. roscheri for all the genetic markers. Given the preferential outbreeding, the high natural fruit set and the pollinator/visitor richness of V. roscheri in Sibaya, a high level of genetic diversity was expected, but this was not the case. We detected a counterintuitive situation between the results of reproductive biology and genetics, making sense once the population situation on the margins of the species distribution is included. The null diversity expressed through the homozygous monomorphic markers is a consequence of the range-edge localization of the populations, which may have been subjected to severe bottlenecks (due to long distance colonization or fragmentation) along with inbreeding, in the past. The analysis highlights the complementarity of the approaches for which the implications in terms of conservation of the species in South Africa are discussed

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Nineteen polymorphic microsatellite markers from two African Vanilla species: across-species transferability and diversity in a wild population of V. humblotii from Mayotte

Cited by 10 publications

References 11 publications

Microsatellite Markers Confirm Self‐Pollination and Autogamy in Wild Populations of Vanilla mexicana Mill. (syn. V. inodora) (Orchidaceae) in the Island of Guadeloupe

Microsatellite Markers Confirm Self‐Pollination and Autogamy in Wild Populations of Vanilla mexicana Mill. (syn. V. inodora) (Orchidaceae) in the Island of Guadeloupe

Drivers of population divergence and species differentiation in a recent group of indigenous orchids (Vanilla spp.) in Madagascar

Active sexual reproduction but no sign of genetic diversity in range-edge populations of Vanilla roscheri Rchb. f. (Orchidaceae) in South Africa

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