Niobium pentoxide as radiopacifying agent of calcium silicate-based material: evaluation of physicochemical and biological properties

Silva, Guilherme F.; Tanomaru-Filho, Mário; Bernardi, Maria Inês Basso; Guerreiro-Tanomaru, Juliane Maria; Cerri, Paulo Sérgio

doi:10.1007/s00784-015-1412-9

Cited by 35 publications

(94 citation statements)

References 31 publications

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“…In fact, the greatest values in the immunoexpression of VEGF and IL-6 were concomitant with chronic inflammatory reaction in the capsules of EF specimens still observed at 60 days. These findings reinforce the concept that the substances released by materials induce the host cells to produce and release several growth factors and cytokines, such as VEGF and IL-6, which drive the fate of the inflammatory reaction 4,5,21 . This concept is reinforced since the IL-6 immunoexpression in the EF group, in the period of 60 days, was similar to the control group at 7 days.…”

Section: Discussionsupporting

confidence: 83%

“…In GFB and MTAF groups, von Kossa-positive structures (in black colour) were observed in the capsules at all time points indicating, therefore, the presence of calcium in the capsules 18,21,37,53,54 . Otherwise, positive structures for calcium were not detected by von Kossa method in the EF and CG groups.…”

Section: Discussionmentioning

confidence: 91%

“…In EF group, a chronic severe/moderate inflammatory reaction with accentuated immunoexpression of IL-6 was still observed in the thick capsules at 60 days. Although calcium silicate-based materials generally promote a moderate/mild inflammatory reaction 4,21,37 , the MTAF contains in its composition resins such as salicylate resin, diluting resin and silica which may be responsible for cytotoxicity promoted by this sealer, mainly, at initial periods 5,35,[38][39][40] . Furthermore, the high solubility about 11-13% of this new MTAF with calcium tungstate 19 may also contribute to the enhanced number of inflammatory cells and IL-6-immunolabelled cells observed in the capsules in comparison with GFB specimens.…”

Section: Discussionmentioning

confidence: 99%

“…The IL-6 is a pro-inflammatory cytokine produced by several cells, including inflammatory cells, which participates in the initiation and maintenance of the inflammatory reaction 25,29,44 . The reduction in the IL-6 immunoexpression culminates in the regression of the inflammatory reaction and collagen formation in the capsules around GFB and MTAF endodontic sealers implanted in the subcutaneous connective tissue 4,5,18,21,22 . Therefore, EF may release harmful substances for a long period leading to a chronic inflammatory process and, consequently, preventing the tissue repair.…”

Section: Discussionmentioning

confidence: 99%

“…The coordinate action of these chemokines regulates the degree of the inflammatory reaction as well as its regression and tissue repair 5 . A biocompatible material may allow the release of mediators by host cells which promote the regression of the inflammatory reaction 4,18,[20][21][22] and stimulate the tissue repair 5,22 . Among the cytokines and growth factors, the interleukin-6 (IL-6) has a participation in the initiation and maintenance of the inflammatory reaction 4,5,[23][24][25] .…”

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confidence: 99%

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Immunoinflammatory response and bioactive potential of GuttaFlow bioseal and MTA Fillapex in the rat subcutaneous tissue

Delfino

Guerreiro-Tanomaru

Tanomaru‐Filho

et al. 2020

Sci Rep

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To evaluate the effect of GuttaFlow bioseal (GFB) and MTA Fillapex (MTAF) in comparison with Endofill (EF) in the subcutaneous tissue. Polyethylene tubes with GFB, MTAF, EF or empty tubes (control group; CG) were implanted into subcutaneous of rats. After 7, 15, 30 and 60 days, the capsule thickness, inflammatory reaction, interleukin-6 (IL-6), vascular endothelial growth factor (VEGF), caspase-3, TUNEL-positive cells, von Kossa and ultrastructural features were evaluated. The data were statistically analyzed (p ≤ 0.05). At all periods, the number of IL-6-and VEGF-immunolabelled cells, and capsule thickness were lower in GFB than MTAF, which was lower than EF (p < 0.0001). At 60 days, the number of inflammatory cells was similar in GFB and MTAF (p = 0.58). Significant differences in the number of TUNEL-and caspase-3-positive cells were not observed among GFB, MTAF and CG whereas the highest values were found in EF specimens. The EF specimens exhibited several cells with condensed chromatin, typical of apoptosis. von Kossa-positive and birefringent structures were only observed in GFB and MTAF, suggesting the presence of calcite crystals. Taken together, these results show that cellular and structural damage induced by GFB and MTAF sealers were recovery over time. Moreover, these sealers express bioactive potential in subcutaneous tissue.The clinical use indication of root canal sealers depends on their physicochemical and biological properties. Thus, the biological properties of endodontic sealers and degree of cytotoxicity on different cells, including fibroblasts, osteoblasts and cementoblasts have been widely investigated 1 . However, in vitro studies have the disadvantage of reflecting the response to a specific cell type and, therefore, do not reflect the host response, which is under influence of several cytokines and growth factors 2 . The implant into subcutaneous connective tissue is widely used to evaluate the biocompatibility and the complex cascade of cellular and molecular events induced by dental materials 2-5 .Studies have suggested that silicone-based endodontic sealers exhibit proper physicochemical and biological properties 6,7 . A novel formulation of silicone-based sealer, the GuttaFlow bioseal (GFB; Coltene Whaledent, GmBH + Co KG, Langenau, Switzerland) is a mixture of gutta-percha powder and polydimethylsiloxane with silver nanoparticles added as a preservative; silica, calcium oxide and phosphorous oxide particles were also combined to this mixture to provide bioactivity and stimulate the tissue repair. The GFB has low solubility and porosity, alkalinizing activity and slight calcium release 8,9 . An in vitro study using human periodontal ligament cells has demonstrated that GFB displays better cytocompatibility than AH Plus 10 , which is considered as a gold standard in the clinical endodontic.Considering the suitable biocompatibility of MTA (Mineral Trioxide Aggregate, Angelus, Londrina, PR, Brazil), the manufactures have added tricalcium silicate to resin-based sealers in an attempt to ...

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Section: Discussionsupporting

confidence: 83%

Section: Discussionmentioning

confidence: 91%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

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Immunoinflammatory response and bioactive potential of GuttaFlow bioseal and MTA Fillapex in the rat subcutaneous tissue

Delfino

Guerreiro-Tanomaru

Tanomaru‐Filho

et al. 2020

Sci Rep

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Bioactive potential of Bio‐C Pulpo is evidenced by presence of birefringent calcite and osteocalcin immunoexpression in the rat subcutaneous tissue

et al. 2022

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As the biocompatibility and bioactive potential of repair materials are desired characteristics in dentistry, the tissue response of Bio‐C Pulpo, a bioceramic material launched on the marked by Angelus (Brazil), was compared with Biodentine (Septodont, France) and White MTA (WMTA; Angelus, Brazil). In 32 rats, 148 polyethylene tubes filled with Bio‐C Pulpo, Biodentine or WMTA, and empty (CG, control group) were implanted into subcutaneous tissues for 7, 15, 30, and 60 days. The capsule thickness, numerical density of inflammatory cells (IC) and fibroblasts (Fb), amount of collagen, immunohistochemistry detection of interleukin‐6 (IL‐6) and osteocalcin (OCN), von Kossa and analysis under polarized light were performed. Data were subjected to two‐way ANOVA followed by Tukey's test (p ≤ 0.05). At 7 and 15 days, the capsules around Bio‐C Pulpo were thicker than in WMTA while, at 30 and 60 days, significant differences were not observed among the groups. Although at 7, 15, and 30 days, a greater number of IL‐6‐immunostained cells was found in Bio‐C Pulpo and Biodentine than in WMTA, no significant difference was detected among the groups at 60 days. In all groups, the number of Fb and collagen content increased significantly over time. The capsules around materials exhibited von Kossa‐positive and birefringent structures, and OCN‐immunostained cells whereas, in the CG, these structures were not observed. Bio‐C Pulpo, similarly to Biodentine and WMTA, is biocompatible, allows the connective tissue repair and presents bioactive potential in connective tissue of rats.

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Perampanel als Therapieoption bei Epilepsiepatienten

Hintz¹,

Nawratil²,

Schulze-Bonhage³

2016

Nervenarzt

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Niobium pentoxide as radiopacifying agent of calcium silicate-based material: evaluation of physicochemical and biological properties

Abstract: Niobium pentoxide can be used as radiopacifier for the development of calcium silicate-based materials.

Cited by 35 publications

References 31 publications

Immunoinflammatory response and bioactive potential of GuttaFlow bioseal and MTA Fillapex in the rat subcutaneous tissue

Immunoinflammatory response and bioactive potential of GuttaFlow bioseal and MTA Fillapex in the rat subcutaneous tissue

Bioactive potential of Bio‐C Pulpo is evidenced by presence of birefringent calcite and osteocalcin immunoexpression in the rat subcutaneous tissue

Perampanel als Therapieoption bei Epilepsiepatienten

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