Niosomal delivery of pumpkin seed oil: development, characterisation, and physical stability

Morakul, Boontida; Teeranachaideekul, Veerawat; Junyaprasert, Varaporn Buraphacheep

doi:10.1080/02652048.2019.1607597

Cited by 16 publications

(10 citation statements)

References 26 publications

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“…It contains a wide variety of bioactive compounds, including fatty acids, phytosterols, vitamins, etc. (12)(13)(14). However, the active ingredients and their mechanisms against BPH are unclear.…”

Section: Popular Scientific Summarymentioning

confidence: 99%

Phytosterols in hull-less pumpkin seed oil, rich in ∆7-phytosterols, ameliorate benign prostatic hyperplasia by lowing 5α-reductase and regulating balance between cell proliferation and apoptosis in rats

Kang

Chen

Zhou

et al. 2021

Food & Nutrition Research

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Background: Pumpkin seed oil is widely used to treat benign prostatic hyperplasia (BPH), a common disease in elder men. However, its active components and mechanism have remained to be elucidated. Objective: The objective of the present study was to investigate the active components of pumpkin seed oil and its mechanism against BPH. Design: Total phytosterol (TPS) was isolated from hull-less pumpkin (Cucurbita pepo L. var. Styriaca) seed oil and analyzed by gas chromatography/mass spectrometry (GC/MS). Three phytosterols were purified by preparative HPLC (high performance liquid chromatography) and confirmed by NMR (nuclear magnetic resonance). TPS (3.3 mg/kg body weight, 1 mL/day/rat) was administered intragastrically to the testosterone propionate-induced BPH rats for 4 weeks. The structure changes of prostate tissues were assessed by hematoxylin & eosin (H&E) staining. The expression of androgen receptor (AR) and steroid receptor coactivator 1 (SRC-1) was analyzed by immunohistochemistry, while that of 5α-reductase (5AR), apoptosis, or proliferation-related growth factors/proteins was detected by real-time quantitative polymerase chain reaction or western blotting. Results: The ∆7-phytosterols in TPS reached up to 87.64%. Among them, 24β-ethylcholesta-7,22,25-trienol, 24β-ethylcholesta-7,25(27)-dien-3-ol, and ∆7-avenasterol were confirmed by NMR. TPS treatment significantly ameliorated the pathological prostate enlargement and restored histopathological alterations of prostate in BPH rats. It effectively suppressed the expressions of 5AR, AR, and coactivator SRC-1. TPS inhibited the expression of proliferation-related growth factor epidermal growth factor, whereas it increased the expressions of apoptosis-related growth factor/gene transforming growth factor-β1. The proliferation-inhibiting effect was achieved by decreasing the ERK (extracellular signal-regulated kinase) phosphorylation, while apoptosis was induced by Caspase 3 activation through JNK (c-Jun N-terminal kinase) and p38 phosphorylation. Conclusion: TPS from hull-less pumpkin seed oil, with ∆7-phytosterols as its main ingredients, is a potential nutraceutical for BPH prevention.

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Section: Popular Scientific Summarymentioning

confidence: 99%

Phytosterols in hull-less pumpkin seed oil, rich in ∆7-phytosterols, ameliorate benign prostatic hyperplasia by lowing 5α-reductase and regulating balance between cell proliferation and apoptosis in rats

Kang

Chen

Zhou

et al. 2021

Food & Nutrition Research

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“…Vegetable oils are the main source of the various phenolic compounds, vitamins, tocopherols, pigments, and minerals that have a positive effect on human health [1]. The essential fatty acids (linoleic and α-linolenic acid) play an important role in brain function, proper growth, and development, reduction of inflammation and risk factors for the development of heart disease, as well as cancer and arthritis therapy [2][3][4]. The plum seeds have significant amounts of oil (about 30%) [5] that contains various bioactive compounds, including tocols, phenolic compounds [6], proteins, and lipids [7].…”

Section: Introductionmentioning

confidence: 99%

Physico-Chemical Properties and Oxidative Stability of Fixed Oil from Plum Seeds (Prunus domestica Linn.)

Savić

Gajić

2020

Biomolecules

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Storage of a great amount of plum kernel waste becomes a challenge for food industry. In this work, the plum seed was used as a source of fixed oil that can be an ingredient of commercial products. Soxhlet extraction was carried out using the different solvents, such as n-hexane, n-heptane, ethyl acetate, acetone, or chloroform:methanol mixture (2:1 v/v). The highest yield of oil (about 30%) was obtained using n-heptane and n-hexane, while the lowest yield was obtained using ethyl acetate. The analysis of physico-chemical parameters indicated that all samples of plum seed oil have an exceptional quality. Schaal oven test indicated that the fixed oil of plum seed exhibited satisfactory oxidative stability at moderate storage temperatures (up to 65 °C). The composition of phenolic compounds in the oil samples was determined using HPLC method. The most abundant compound of seven identified and quantified phenolic compounds was vanillic acid. The highest content of β-carotene (1.67 mg 100 g−1 fixed oil) spectrophotometrically determined was in the oil extracted with n-hexane. The lowest content of β-carotene (1.26 mg 100 g−1 fixed oil) was determined in the oil extracted with a mixture of chloroform:methanol (2:1 v/v). This oil had the highest antioxidant activity (IC50 value of 4.35 mg mL−1) compared to other oil samples. The antioxidant activity was probably caused by the presence of phenolic compounds. The investigated physico-chemical properties demonstrated that the plum seed oil has a potential for application in the food, cosmetics, and pharmaceutical industry.

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“…Here, the concentration of the drug released is correlated to the square root of time with the slope of k (mg L −1 min −1/2 ). [ 35 ]…”

Section: Resultsmentioning

confidence: 99%

Inclusion of sulfamethoxazole in a novel CuFe₂O₄ nanoparticles/mesoporous silica‐based nanocomposite: Release kinetics and antibacterial activity

Mehrabi

Shamspur

Mostafavi

et al. 2020

Applied Organom Chemis

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In this work, we aimed to improve the antibacterial activity of sulfamethoxazole (SMX) via its inclusion in a newly synthesized nanocomposite composed of CuFe 2 O 4 nanoparticles and poly(vinyl alcohol)/silica-based mesoporous materials. Characterization of this formulation using different techniques confirmed the correct synthesis and showed that this mesoporous nanocomposite had an amorphous structure with relatively high surface area of 1,620.7 m 2 g −1 and mean pore diameter of 1.6576 nm. Zeta potential of the formulation was obtained to be zero which led to its higher bioavailability in comparison to pure SMX with negative zeta potential. Antibacterial property of the prepared formulation against Staphylococcus aureus and Escherichia coli was evaluated. Minimum inhibitory concentration of the SMX-loaded mesoporous nanocomposite was considerably lower than those of pure SMX, indicating the efficient function of the mesoporous material as a delivery system. Kinetics of SMX release was also studied using zero-order, first-order, Higuchi, and Korsmeyer-Peppas kinetics models. According to the obtained results, the release kinetics was found to obey zero-order model. So the possibility of sustained release of SMX from the synthesized carrier may be suggested.

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Niosomal delivery of pumpkin seed oil: development, characterisation, and physical stability

Cited by 16 publications

References 26 publications

Phytosterols in hull-less pumpkin seed oil, rich in ∆7-phytosterols, ameliorate benign prostatic hyperplasia by lowing 5α-reductase and regulating balance between cell proliferation and apoptosis in rats

Phytosterols in hull-less pumpkin seed oil, rich in ∆7-phytosterols, ameliorate benign prostatic hyperplasia by lowing 5α-reductase and regulating balance between cell proliferation and apoptosis in rats

Physico-Chemical Properties and Oxidative Stability of Fixed Oil from Plum Seeds (Prunus domestica Linn.)

Inclusion of sulfamethoxazole in a novel CuFe₂O₄ nanoparticles/mesoporous silica‐based nanocomposite: Release kinetics and antibacterial activity

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Niosomal delivery of pumpkin seed oil: development, characterisation, and physical stability

Cited by 16 publications

References 26 publications

Phytosterols in hull-less pumpkin seed oil, rich in ∆7-phytosterols, ameliorate benign prostatic hyperplasia by lowing 5α-reductase and regulating balance between cell proliferation and apoptosis in rats

Phytosterols in hull-less pumpkin seed oil, rich in ∆7-phytosterols, ameliorate benign prostatic hyperplasia by lowing 5α-reductase and regulating balance between cell proliferation and apoptosis in rats

Physico-Chemical Properties and Oxidative Stability of Fixed Oil from Plum Seeds (Prunus domestica Linn.)

Inclusion of sulfamethoxazole in a novel CuFe2O4 nanoparticles/mesoporous silica‐based nanocomposite: Release kinetics and antibacterial activity

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Product

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Inclusion of sulfamethoxazole in a novel CuFe₂O₄ nanoparticles/mesoporous silica‐based nanocomposite: Release kinetics and antibacterial activity