Niosome-Encapsulated Gentamicin for Ophthalmic Controlled Delivery

Abstract

Abstract. The objective of the present research was to investigate the feasibility of using non-ionic surfactant vesicles (niosomes) as carriers for the ophthalmic controlled delivery of a water soluble local antibiotic; gentamicin sulphate. Niosomal formulations were prepared using various surfactants (Tween 60, Tween 80 or Brij 35), in the presence of cholesterol and a negative charge inducer dicetyl phosphate (DCP) in different molar ratios and by employing a thin film hydration technique. The ability of these vesicles to entrap the studied drug was evaluated by determining the entrapment efficiency %EE after centrifugation and separation of the formed vesicles. Photomicroscopy and transmission electron microscopy as well as particle size analysis were used to study the formation, morphology and size of the drug loaded niosomes. Results showed a substantial change in the release rate and an alteration in the %EE of gentamicin sulphate from niosomal formulations upon varying type of surfactant, cholesterol content and presence or absence of DCP. In-vitro drug release results confirmed that niosomal formulations have exhibited a high retention of gentamicin sulphate inside the vesicles such that their in vitro release was slower compared to the drug solution. A preparation with 1:1:0.1 molar ratio of Tween 60, cholesterol and DCP gave the most advantageous entrapment (92.02%±1.43) and release results (Q 8h =66.29%±1.33) as compared to other compositions. Ocular irritancy test performed on albino rabbits, showed no sign of irritation for all tested niosomal formulations.

PEGylated non-ionic surfactant vesicles as drug delivery systems for Gambogenic acid

“…In this study, a comparison has been made between spans-and Tweens-loading GNA, the results of the DLS study are shown in Table 1. The studies with the sizes gave us a qualitative idea of the niosomal delivery systems prepared using Spans smaller in the size than that of Tweens, the similar results were reported for Gentamicin niosomes (Abdelbary & El-gendy, 2008). This finding may be due to Spans with shorter alkyl chains and lower HLB values than Tweens, therefore causing a reduction of the repulsion forces between the hydroxyl groups of Spans and the carboxyl groups of the anticancer drug, thus it should be able to produce smaller size PEGylated niosomes.…”

Niosomes: A Controlled and Novel Drug Delivery System

“…Also, as compared to normal drug solution, niosomes of drug show slow release. 57) Niosomal formulation containing timolol meleate (0.25%) prepared by chitosan coating exhibited more effect on intra ocular tension with fewer side effects as compared to the marketed formulation. …”

Solid lipid nanoparticles for ocular drug delivery

“…Niosomes have been used to deliver drugs like gentamicin to the eye (Abdelbary & El-gendy, 2008). Niosomes are formed from the self-assembling of nonionic amphiphiles in aqueous medium which are able to accommodate hydrophilic and hydrophobic drugs (Carafa et al, 1998;Uchegbu & Vyas, 1998;Abdelbary & El-gendy, 2008).…”

“…Niosomes are formed from the self-assembling of nonionic amphiphiles in aqueous medium which are able to accommodate hydrophilic and hydrophobic drugs (Carafa et al, 1998;Uchegbu & Vyas, 1998;Abdelbary & El-gendy, 2008). Niosomal ocular drug delivery concept aims to enhance periocular retention, corneal penetration, and provide controlled release in a similae manner to SLNs and liposomes.…”