NIR-II Dyad-Doped Ratiometric Nanosensor with Enhanced Spectral Fidelity in Biological Media for In Vivo Biosensing

Yu, Peng; Yan, Kui; Wang, Shangfeng; Yao, Chenzhi; Lei, Zuhai; Tang, Yaohui; Zhang, Fan

doi:10.1021/acs.nanolett.2c04084

Cited by 18 publications

(6 citation statements)

References 58 publications

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“…Here, we addressed a fundamental concern of making single nanoparticle ratiometric sensors: the uncontrollable ratio between different encapsulated fluorescent dye molecules. It should lead to a variation of fluorescence spectra between different particles [ 1 , 2 , 3 , 4 ]. The majority of ratiometric sensors deal with two fluorescent dyes (one as a reference and the other dye as the sensing one) [ 5 , 7 , 9 , 11 ].…”

Section: Discussionmentioning

confidence: 99%

“…For many years, concentrations of ions and acidity were measured using macroscopic methods such as titration, litmus paper, or electrochemical cells. However, the spatial and temporal resolutions of those methods prevented the detection of ion concentrations within cells or tissues [ 1 , 2 , 3 ]. Measurements of temperature at the submicron scale were limited too.…”

Section: Introductionmentioning

confidence: 99%

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Towards the Use of Individual Fluorescent Nanoparticles as Ratiometric Sensors: Spectral Robustness of Ultrabright Nanoporous Silica Nanoparticles

Iraniparast

Peng

Sokolov

2023

Sensors

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Here we address an important roadblock that prevents the use of bright fluorescent nanoparticles as individual ratiometric sensors: the possible variation of fluorescence spectra between individual nanoparticles. Ratiometric measurements using florescent dyes have shown their utility in measuring the spatial distribution of temperature, acidity, and concentration of various ions. However, the dyes have a serious limitation in their use as sensors; namely, their fluorescent spectra can change due to interactions with the surrounding dye. Encapsulation of the d, e in a porous material can solve this issue. Recently, we demonstrated the use of ultrabright nanoporous silica nanoparticles (UNSNP) to measure temperature and acidity. The particles have at least two kinds of encapsulated dyes. Ultrahigh brightness of the particles allows measuring of the signal of interest at the single particle level. However, it raises the problem of spectral variation between particles, which is impossible to control at the nanoscale. Here, we study spectral variations between the UNSNP which have two different encapsulated dyes: rhodamine R6G and RB. The dyes can be used to measure temperature. We synthesized these particles using three different ratios of the dyes. We measured the spectra of individual nanoparticles and compared them with simulations. We observed a rather small variation of fluorescence spectra between individual UNSNP, and the spectra were in very good agreement with the results of our simulations. Thus, one can conclude that individual UNSNP can be used as effective ratiometric sensors.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Towards the Use of Individual Fluorescent Nanoparticles as Ratiometric Sensors: Spectral Robustness of Ultrabright Nanoporous Silica Nanoparticles

Iraniparast

Peng

Sokolov

2023

Sensors

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“…Among them, organic molecules have a lot of advantages, such as tunable NLO properties through the design of molecular structures, excellent biocompatibility and low fabrication cost [16,17]. Compared with the molecules with visible absorption, near-infrared (NIR) ones have important applications in communication, biological and security [18,19]. For example, the NIR molecules with strong MPA have deep penetration and high resolution in fluorescence imaging [20,21].…”

Section: Introductionmentioning

confidence: 99%

Ultrafast Nonlinear Optical Activities of Near-Infrared Aza-BODIPY Derivatives

Ren¹,

Xiao²,

Cui³

et al. 2023

Preprint

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The near-infrared (NIR) organic dyes with strong ultrafast nonlinear optical (NLO) activities are of importance for various applications. However, such kinds of dyes are still scarce. In this work, we have compared the NLO properties of two NIR Aza-BODIPY derivatives, in which the strong electron-donating groups, namely 4-(N, N-dimethylamino) phenyl and 1-ethyl-1,2,3,4-tetrahydroquinoline groups, are connected with the cores of Aza-BODIPY. Z-Scan experimental results show that two Aza-BODIPY derivatives exhibit strong saturation absorption and large modulation depth under the excitation of femtosecond pulses at 800 nm. Under 1300 nm excitation, two derivatives exhibit strong nonlinear refraction. In addition, the Aza-BODIPY derivatives also display effective two-photon excited fluorescence emission in the wavelength range of 1200-1600 nm. Based on the experimental results, it is found that 1-ethyl-1,2,3,4-tetrahydroquinoline group can more effectively enhance the NLO properties of Aza-BODIPY derivatives compared with the 4-(N, N-dimethylamino) phenyl group, thus providing new possibilities for the design and development of NIR NLO materials.

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“…Driven by the clinical needs, a wide range of in vivo probes have been explored, including nanoparticles, − small-molecule dyes, − etc. Among these materials, semiconducting polymer dots (Pdots) have been demonstrated as a class of promising fluorescent probes due to their excellent photostability, high single-particle brightness, good biocompatibility, etc. − Though Pdots with NIR-II emission have been reported, − their optical properties, including the absorption cross-section and the fluorescence quantum yield (QY), still require further optimization.…”

mentioning

confidence: 99%

Hyperbranched Polymer Dots with Strong Absorption and High Fluorescence Quantum Yield for In Vivo NIR-II Imaging

Fang,

Ai,

Wang

et al. 2023

Nano Lett.

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In order to improve the fluorescence quantum yield (QY) of NIR-II-emitting nanoparticles, D-A-D fluorophores are typically linked to intramolecular rotatable units to reduce aggregation-induced quenching. However, incorporating such units often leads to a twisted molecular backbone, which affects the coupling within the D-A-D unit and, as a result, lowers the absorption. Here, we overcome this limitation by cross-linking the NIR-II fluorophores to form a 2D polymer network, which simultaneously achieves a high QY by well-controlled fluorophore separation and strong absorption by restricting intramolecular distortion. Using the strategy, we developed polymer dots with the highest NIR-II single-particle brightness among reported D-A-D-based nanoparticles and applied them for imaging of hindlimb vasculatures and tumors as well as fluorescence-guided tumor resection. The high brightness of the polymer dots offered exceptional image quality and excellent surgical results, showing a promising performance for these applications.

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NIR-II Dyad-Doped Ratiometric Nanosensor with Enhanced Spectral Fidelity in Biological Media for In Vivo Biosensing

Cited by 18 publications

References 58 publications

Towards the Use of Individual Fluorescent Nanoparticles as Ratiometric Sensors: Spectral Robustness of Ultrabright Nanoporous Silica Nanoparticles

Towards the Use of Individual Fluorescent Nanoparticles as Ratiometric Sensors: Spectral Robustness of Ultrabright Nanoporous Silica Nanoparticles

Ultrafast Nonlinear Optical Activities of Near-Infrared Aza-BODIPY Derivatives

Hyperbranched Polymer Dots with Strong Absorption and High Fluorescence Quantum Yield for In Vivo NIR-II Imaging

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