NIR triggered glycosylated gold nanoshell as a photothermal agent on melanoma cancer cells

Abstract

Nowadays, gold nanoshells are used in targeted nano photothermal cancer therapy. This study surveyed the application of gold nanoshell (GNs) to thermal ablative therapy for melanoma cancer cells and it takes advantage of the near infrared absorption of gold nanoshells. The synthesis and characterization of glycosylated gold nanoshells (GGNs) were done. The cytotoxicity and photothermal effects of GNs on melanoma cells were evaluated using MTT assay and flow cytometry. The characterization data showed that GGNs are spherical, with a hydrodynamic size of 46.7 nm. Results suggest that the cellular uptake of GGNs was about 78%. Viability assays showed no significant toxicity at low concentrations of GNs. The higher heating rate and toxicity of cancer cells were obtained for the cells exposed to 808 nm NIR laser after incubation with GGNs rather than the GNs. The viability of these cells has dramatically decreased by 29%. Furthermore, 61% more cell lethality was achieved for A375 cells using combined photothermal therapy and treatment with GGNs in comparison to NIR radiation alone. In conclusion, our findings suggest that the synthesized gold/silica core-shell nanoparticles conjugated with glucosamine have high potentials to be considered as an efficient metal-nanoshell in the process of targeted cancer photothermal therapy.

Near-infra-red fluorescent chitosan oligosaccharide lactate for targeted cancer imaging and photothermal therapy

“…To date, a number of technologies for cancer treatments have been developed including chemotherapy, radiotherapy, photodynamic therapy, photothermal therapy (PTT), and combination therapy. Among these options, PTT has great potential for effectively treating cancers because of its noninvasive procedure, low toxicity to normal tissues, and high tumour ablation efficiency [1][2][3][4][5][6][7]. Photothermal therapeutic agents that exhibit strong absorbance in the near-infra-red (NIR) region can convert light energy into heat energy to induce hyperthermia (>48 C) and kill tumour cells [8][9][10][11][12].…”

Photothermal conversion of SiO2@Au nanoparticles mediated by surface morphology of gold cluster layer

“…42 For multi-component core-shell gold nanomaterials, more attention has been paid on the photothermal conversion capability of the gold shell. [43][44][45][46] In this work, we study the effects of the morphology of gold clusters on the surface of SiO 2 @Au core-shell nanoparticles on their photothermal conversion performance by changing the amount of gold salt, the pH value of the growth solution, and the volume and concentration of the reducing agent used in the chemical synthesis of SiO 2 @Au. We introduce the equivalent circle diameter to characterize the size of irregular gold clusters on the surface of SiO 2 and measure the direct shortest distance between the irregular gold clusters using Image J.…”

Recent Advances in Nanoscale Metal–Organic Frameworks Towards Cancer Cell Cytotoxicity: An Overview

“…synthesized gold coated silica NPs, which they injected into A365 tumor cells [ 57 ]. A four-minute irradiation (808 nm, 0.9 W/cm 2 ) caused cell viability to drop to 31% [ 58 ]. Other reports on core–shell gold structures show similar in vitro cytotoxicity results, where KB cancer cell death reached 60% after irradiation (755–808 nm; 6–40 W/cm 2 ) [ 59 , 60 ].…”