NirS-type N2O-producers and nosZ II-type N2O-reducers determine the N2O emission potential in farmland rhizosphere soils

Zhao, Shuai; Zhou, Jiemin; Yuan, Dongdan; Wei-dong, Wang; Zhou, Lei; Pi, Yanxia; Zhu, Guibing

doi:10.1007/s11368-019-02395-3

Cited by 35 publications

(13 citation statements)

References 42 publications

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“…The evident enrichment of the nosZ gene in PC-OH was revealed with the maximum ratio of ∑ nos / 16S rRNA . Previous studies have shown that the quantitative balance between the N 2 O-producing microorganisms ( nirS and nirK ) and N 2 O-reducing microorganisms ( nosZ I and nosZ II) regulated the net N 2 O emission to some extent [ 43 ], and the lower ratios of ∑ nir /∑ nos suggested the more complete denitrification with less N 2 O emissions [ 44 ]. In this study, the minimum ratios of ∑ nir /∑ nos in PC-OH (5.27) positively correlated with N 2 O net accumulation rates and corresponded to the results of Kong et al [ 45 ] and Saarenheimo et al [ 46 ].…”

Section: Resultsmentioning

confidence: 99%

Improved Denitrification Performance of Polybutylene Succinate/Corncob Composite Carbon Source by Proper Pretreatment: Performance, Functional Genes and Microbial Community Structure

et al. 2023

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Blending biodegradable polymers with plant materials is an effective method to improve the biodegradability of solid carbon sources and save denitrification costs, but the recalcitrant lignin in plant materials hinders the microbial decomposition of available carbon sources. In the present study, corncob pretreated by different methods was used to prepare polybutylene succinate/corncob (PBS/corncob) composites for biological denitrification. The PBS/corncob composite with alkaline pretreatment achieved the optimal NO3−-N removal rate (0.13 kg NO3−-N m−3 day−1) with less adverse effects. The pretreatment degree, temperature, and their interaction distinctly impacted the nitrogen removal performance and dissolved organic carbon (DOC) release, while the N2O emission was mainly affected by the temperature and the interaction of temperature and pretreatment degree. Microbial community analysis showed that the bacterial community was responsible for both denitrification and lignocellulose degradation, while the fungal community was primarily in charge of lignocellulose degradation. The outcomes of this study provide an effective strategy for improving the denitrification performance of composite carbon sources.

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Section: Resultsmentioning

confidence: 99%

Improved Denitrification Performance of Polybutylene Succinate/Corncob Composite Carbon Source by Proper Pretreatment: Performance, Functional Genes and Microbial Community Structure

et al. 2023

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“…It is pertinent to mention here that the addition of plant litter might have triggered the community enrichment between the AOA and AOB, with AOB being the dominant actors for nitrification crucial in is estimating the overall nitrification rates [49]. In DEA nosZ were less abundant, but due to their dominant role as shown by correlation analyses and involvement in reduction of N 2 O to N 2 these are critical in deciding the overall nitrous oxide budget from soils [38,50].…”

Section: Discussionmentioning

confidence: 99%

Assessing the N Cycling Ecosystem Function-Processes and the Involved Functional Guilds upon Plant Litter Amendment in Lower Himalaya

Zaman¹,

Iqbal²,

Shaukat³

et al. 2020

Pol. J. Environ. Stud.

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Nitrogen (N) cycling ecosystem function is crucial in primary productivity but also carry various ecological implications such as N losses to environment. In mountainous soil ecosystems, this important function beside soil characteristics is dependent on the litter produced through plant decay which may play a critical role in shaping the hosted soil microbial communities such as those involved in N cycling processes. This study aims at investigating the effects of plant litter amendment, believed to reduce the nitrogen leaching and improve soil health, on nitrogen cycling microbial communities and processes using litterbag approach at field station of COMSATS, Abbottabad. Plant litter collected from the stands of Pine (Pinus wallichiana) and understory Indigo shrub commonly known as Indigo Himalayan (Indigofera heterentha (Fabaceae) wall), near Abbottabad, native to the lower Himalaya, were applied to indigenous loamy soil in four treatments (i.e. Control, Pine, Indigo and Pine + Indigo). The N cycling processes (involved in nitrous oxide GHG emissions, potential nitrification activity -PNA and denitrification enzyme activity -DEA), through measuring enzymes activities and the abundances of nitrifying -amoA (ammonia oxidizing bacteria -AOB, ammonia oxidizing archaea -AOA) and denitrifying functional guilds (nirS, nosZ) were determined using quantitative PCRs by targeting their corresponding genes. The results revealed that the plant litter significantly influenced both nitrification and denitrification but also the size of microbial communities involved in these two

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“…34 It was reported that the clade II NosZ gene was widely found in Dechloromonas, presumably contributing to N 2 O reduction due to its higher affinity for N 2 O. 15,35 An acetate-enriched denitrifying culture possessing Dechloromonas as the main N 2 O reducer exhibited greater N 2 O reduction than that of methanol, which mainly possessed Methylotenera under non-carbon limiting conditions. 13 It was consistent with results in Figure 1C, where the abundance of Dechloromonas (i.e., 14.1%) for SA-denitrifiers was higher than that of Methylotenera (i.e., 8.1%) for ME-denitrifiers.…”

Section: ■ Discussionmentioning

confidence: 99%

Selective Organic Carbon Enrichment Influences Nitrous Oxide Reduction by Denitrifiers: Electron Competition Insights

Liu

Ren

et al. 2022

ACS EST Water

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A promising approach to reduce nitrous oxide (N 2 O) emission from wastewater treatment plants (WWTPs) would be to enrich highly efficient heterotrophic denitrifiers that are capable of regulating N 2 O reduction. The specific denitrifying community structure could be influenced by the types of organic carbon fed, while their corresponding N 2 O reduction capacity and electron competition mechanism remain unclear. In this study, the N 2 O reduction capacity by methanol-, ethanol-, and sodium acetate-enriched denitrifiers was systematically explored at varying carbon-loading rates. The effect of electron competition on N 2 O reduction was investigated by the combination of the microbial community and biochemical experiments. The results indicated that sodium acetate-enriched denitrifiers harboring abundant N 2 O reductase (Nos) genes invariably maintained the highest N 2 O reduction rate (r N 2 O ). However, the concurrent presence of multiple electron acceptors (i.e., NO 3 − and/or NO 2 − ) resulted in the higher susceptibility of r N 2 O for sodium acetate-enriched denitrifiers, which could explain the decrease in the N 2 O reduction rate under sufficient carbon supply. However, under insufficient organic carbon supply, Nos was confronted with fiercer electron competition from the upstream electron pool, causing more N 2 O accumulation. Moreover, a quantitative investigation of the electron distribution corroborated the aforementioned N 2 O reduction data. Further studies are warranted to optimize organic carbon-feeding strategies to utilize denitrifiers as an effective N 2 O sink at WWTPs.

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NirS-type N2O-producers and nosZ II-type N2O-reducers determine the N2O emission potential in farmland rhizosphere soils

Cited by 35 publications

References 42 publications

Improved Denitrification Performance of Polybutylene Succinate/Corncob Composite Carbon Source by Proper Pretreatment: Performance, Functional Genes and Microbial Community Structure

Improved Denitrification Performance of Polybutylene Succinate/Corncob Composite Carbon Source by Proper Pretreatment: Performance, Functional Genes and Microbial Community Structure

Assessing the N Cycling Ecosystem Function-Processes and the Involved Functional Guilds upon Plant Litter Amendment in Lower Himalaya

Selective Organic Carbon Enrichment Influences Nitrous Oxide Reduction by Denitrifiers: Electron Competition Insights

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