Nisin anchored cellulose nanofibers for long term antimicrobial active food packaging

Abstract

Increasing consumer demand for high performance bio-based materials in order to develop microbiologically safer foods has forced the food industry to revise their packaging strategies.

Cellulose nanofiber (CNF)–sakacin‐A active material: production, characterization and application in storage trials of smoked salmon

“…Different strategies for the conjugation or grafting of antimicrobial compounds on CNF have been described in the literature but their efficacy is closely connected to the active molecule (aminosilanes, antibiotics, bacteriocins, etc.). 17,23,37 The set-up of the CNF-sakacin-A active materials was found effective in reducing Listeria population in samples of smoked salmon of about 3 Log cycles with respect to the values reached without or with CNF-only samples after 28 days of storage. In the applied conditions, the bacteriocin concentration in mats was around 50 AU cm −2 .…”

“…Barbiroli et al reported the possibility of incorporating lysozyme and lactoferrin into paper containing carboxymethyl cellulose, that allowed non‐covalent binding of the positively charged proteins to the paper matrix; tests on thin cuts of raw meat also confirmed their antimicrobial effect. Saini et al developed a novel antimicrobial film with covalently linked nisin on surface of TEMPO oxidized cellulose nanofibers for food packaging. Espitia et al studied the effects of pediocin incorporation into a cellulosic packaging produced with cellulose acetate resin, determining the tensile strength at break (in MPa), load at break (in newtons) and elongation at break (%), water vapor permeability and structure.…”

“…In the past decade, nanomaterial of cellulose has been developed, named cellulose nanofibers (CNFs), with diameters between 10 and 50 nm and lengths of several millimeters. This novel material can enhance mechanical and barrier properties when applied in packaging materials . The use of cellulose nanocomposites in food packaging can help extend shelf‐life and enhance food quality, since they serve not only as barrier to moisture, water vapor, gases, and solutes but they can also be considered carriers of active substances such as antimicrobials …”

“…This novel material can enhance mechanical and barrier properties when applied in packaging materials. 17,26 The use of cellulose nanocomposites in food packaging can help extend shelf-life and enhance food quality, since they serve not only as barrier to moisture, water vapor, gases, and solutes but they can also be considered carriers of active substances such as antimicrobials. 25 In the present study, a protein extract containing the bacteriocin sakacin-A, produced by Lactobacillus sakei Lb 706 in a low-cost culture medium, was adsorbed onto CNFs to obtain an antimicrobial material.…”

New perspectives in active and intelligent food packaging

“…In their studies on developing an antimicrobial active packaging film, Riaz et al (2018) Many authors developed antimicrobial active packaging with the use of nisin. Nisin has already been used as an antibacterial agent in food products for the past few decades and also has been approved by the European food safety authority within acceptable uptake (Saini, Sillard, Belgacem, & Bras, 2016). Nisin is a bacteriocin and an antimicrobial peptide with 34 amino acids and a molecular weight of 3.5 kDa, produced by strains of Lactococus lactissubsp.…”