Nisin-Controlled Extracellular Production of Interleukin-2 in<i>Lactococcus lactis</i>Strains, without the Requirement for a Signal Peptide Sequence

Fernández, Antonio José Díaz; Rodríguez, Juan M.; Bongaerts, Roy J.; Gasson, Michael J.; Horn, Nikki

doi:10.1128/aem.01247-07

Cited by 10 publications

(2 citation statements)

References 23 publications

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“…For example, Steidler et al [ 71 ] constructed a vector capable of expressing mIL-2 in Lactococcus lactis under the controll phage promoter T7, where the cytokine expression cassette was fused to the usp 45 gene [ 71 ]. Fernández et al [ 72 ] showed that Lactococcus lactis has the potential to secrete mIL-2 under the control of the nisin promoter pnisA without the need for a signal peptide, and the expression was confirmed by Western blot technique [ 72 ].…”

Section: Immune System and Cytokinesmentioning

confidence: 99%

Lactococcus lactis as an Interleukin Delivery System for Prophylaxis and Treatment of Inflammatory and Autoimmune Diseases

Campos

Américo

Freitas

et al. 2023

Probiotics & Antimicro. Prot.

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Target delivery of therapeutic agents with anti-inflammatory properties using probiotics as delivery and recombinant protein expression vehicles is a promising approach for the prevention and treatment of many diseases, such as cancer and intestinal immune disorders. Lactococcus lactis , a Lactic Acid Bacteria (LAB) widely used in the dairy industry, is one of the most important microorganisms with GRAS status for human consumption, for which biotechnological tools have already been developed to express and deliver recombinant biomolecules with anti-inflammatory properties. Cytokines, for example, are immune system communication molecules present at virtually all levels of the immune response. They are essential in cellular and humoral processes, such as hampering inflammation or adjuvating in the adaptive immune response, making them good candidates for therapeutic approaches. This review discusses the advances in the development of new therapies and prophylactic approaches using LAB to deliver/express cytokines for the treatment of inflammatory and autoimmune diseases in the future.

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Section: Immune System and Cytokinesmentioning

confidence: 99%

Lactococcus lactis as an Interleukin Delivery System for Prophylaxis and Treatment of Inflammatory and Autoimmune Diseases

Campos

Américo

Freitas

et al. 2023

Probiotics & Antimicro. Prot.

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“…The treatment with probiotics can modulate the immune responses in the lung, which increases the regulatory T cell response in the treatment of PBMCs and macrophages with combined M. tuberculosis and LAB. 58 , 59 …”

Section: Bacterial Natural Compounds With Anti-inflammatory Activitiementioning

confidence: 99%

<p>Bacterial Natural Compounds with Anti-Inflammatory and Immunomodulatory Properties (Mini Review)</p>

Jenab¹,

Roghanian²,

Emtiazi³

2020

DDDT

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Inflammation is part of the body’s complex biological response to harmful stimuli such as damaged cells, pathogens, or irritants. It is a protective response involving blood cells, immune cells, and molecular mediators. The inflammation not only can eliminate the primary cause of cell injury but also clears out necrotic cells, tissue damaged from the original insults and inflammatory process. Furthermore, it can initiate tissue repair. Pro-inflammatory cytokines are produced predominantly by activated macrophages and are involved in the up-regulation of inflammatory reactions. They are involved in further regulating inflammatory reactions. There is ample evidence that some pro-inflammatory cytokines, such as interleukin 1β (IL-1β), IL-6, and tumor necrosis factor-α (TNF-α), are involved in the pathological pain process. Some of the natural compounds promote cytokines production and inhibit inflammatory responses. The natural compounds which are produced from microorganisms such as omega-3 fatty acid, cyclic peptide, antimicrobial peptide, oligosaccharides, and polysaccharides can reduce inflammation and could be easily incorporated into the diet without any adverse effects. For example, SCFA (short-chain fatty acids), peptide bacteriocin, and polycyclic peptide bacteriocin (nisin) could be used in the treatment of atherosclerosis, orthopedic postoperative infections, and mycobacterium tuberculosis infection, respectively. Also, fatty acids (saturated and unsaturated fatty acids) can be introduced as anti-inflammatory drugs. This review article summarizes bacterial natural compounds with modulating effects on cytokines that are surveyed which may have potential anti-inflammatory drug-like activity.

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In vitro activity of a recombinant ABC transporter protein in the processing of plantaricin E pre-peptide

Pal

Srivastava

2015

Arch Microbiol

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Most bacteriocins of lactic acid bacteria (LAB) are initially synthesized as pre-peptides with an N-terminal extension (leader peptides). Generally, the precursor peptides containing a double-glycine-type leader are processed by a dedicated ATP-binding cassette (ABC) transporter. The ABC transporter and an accessory protein lead to the cleavage of inactive pre-peptide with the concomitant export of the mature peptide across the cytoplasmic membrane. Plantaricins E, F, J, and K belong to class IIb 2-peptide bacteriocins and are synthesized as pre-peptides containing N-terminal G-G leader peptide. In this study, the heterologous expression, purification, and characterization of PlnE pre-peptide, ABC transporter (PlnG), and accessory protein (PlnH) from Lactobacillus plantarum LR/14 in Escherichia coli BL21 (DE3) strain were reported. An in vitro assay was conducted with the inactive PlnE pre-peptide, which after cleavage by the addition of ABC transporter protein exhibited antimicrobial activity against some LAB species. The activity of cleaved pre-peptide was comparable to the activity of mature peptide. Accessory protein was also heterologously expressed and purified; however, no effect on processing activity was detected by the addition of the accessory protein, which suggests that accessory protein is not involved in cleavage, but it might help in the transport of mature plantaricins across the membrane.

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Nisin-Controlled Extracellular Production of Interleukin-2 inLactococcus lactisStrains, without the Requirement for a Signal Peptide Sequence

Cited by 10 publications

References 23 publications

Lactococcus lactis as an Interleukin Delivery System for Prophylaxis and Treatment of Inflammatory and Autoimmune Diseases

Lactococcus lactis as an Interleukin Delivery System for Prophylaxis and Treatment of Inflammatory and Autoimmune Diseases

<p>Bacterial Natural Compounds with Anti-Inflammatory and Immunomodulatory Properties (Mini Review)</p>

In vitro activity of a recombinant ABC transporter protein in the processing of plantaricin E pre-peptide

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