1990
DOI: 10.1073/pnas.87.14.5331
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nit-2, the major positive-acting nitrogen regulatory gene of Neurospora crassa, encodes a sequence-specific DNA-binding protein.

Abstract: The nit-2 major nitrogen regulatory gene of Neurospora crassa turns on the expression of various unlinked structural genes that specify nitrogen-catabolic enzymes under nitrogen-limitation conditions. The nit-2 gene encodes a protein of 1036 amino acid residues with a single zinc finger and a downstream basic region that may make up a DNA-binding domain. The zinc-finger domain of the NIT2 protein was synthesized in two ways to examine its DNA-binding activity with gel-band-mobility shift and DNA-footprint expe… Show more

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Cited by 229 publications
(133 citation statements)
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“…Each site contains two or more GATA core elements, which were found to be essential for NIT2 binding, although bases flanking the GATA sequences seemed to make only minor contributions to binding-site strength (1,2). Three NIT2 binding sites (A, B, and E) have been identified in the upstream promoter region of the nit-3 gene by EMSA and footprinting experiments with a NIT2-␤-galactosidase fusion protein (7). Site A contains three GATA elements, site B contains only a single GATA, and site E has four GATA sequences (Fig.…”
Section: Resultsmentioning
confidence: 99%
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“…Each site contains two or more GATA core elements, which were found to be essential for NIT2 binding, although bases flanking the GATA sequences seemed to make only minor contributions to binding-site strength (1,2). Three NIT2 binding sites (A, B, and E) have been identified in the upstream promoter region of the nit-3 gene by EMSA and footprinting experiments with a NIT2-␤-galactosidase fusion protein (7). Site A contains three GATA elements, site B contains only a single GATA, and site E has four GATA sequences (Fig.…”
Section: Resultsmentioning
confidence: 99%
“…These DNA fragments were end labeled with 32 P by 3Ј fill-in with DNA polymerase I Klenow fragment. The 32 P-labeled DNA probes were incubated with nuclear extract containing native NIT2 protein and electrophoresed under conditions described previously (7). The gels were dried and scanned with a Betascope 603 blot analyzer (Betagen, Boston, Mass.).…”
Section: Methodsmentioning
confidence: 99%
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“…nitrate, purines or amino acids, is subject to nitrogen metabolic repression (Marzluf, 1996;1997). Expression of the genes that specify nitrogen catabolic enzymes in N. crassa is mediated by NIT2, a global-acting factor that has a single zinc finger DNA-binding domain and is a member of the GATA family of regulatory proteins (Fu and Marzluf, 1990a). GATA factors contain one or two Cys 2 /Cys 2 -type zinc fingers, each with a central loop of either 17 or 18 amino acids; these factors bind DNA elements that possess a GATA core sequence.…”
Section: Introductionmentioning
confidence: 99%
“…AreA is a transcription factor of GATA type, which participates in nitrogen metabolism regulation in lower fungi (Grove and Marzluf 1981;Davis and Hynes 1987;Fu and Marzluf 1990;Marzluf 1997;Andrianopoulos et al 1998;Rutter et al 2001). In fungi, NmrA protein can regulate AreA transcription factor either by direct inhibition of its binding on DNA or indirectly by controlling entry of this factor into nucleus on the basis of its redox status (Fu and Marzluf 1990;Wilson and Arst 1998).…”
Section: Discussionmentioning
confidence: 99%