Nitrate and nitrite bacterial reduction at alkaline pH and high nitrate concentrations, comparison of acetate versus dihydrogen as electron donors

Albina, Pierre; Durban, Nadège; Bertron, Alexandra; Albrecht, Achim; Robinet, Jean-Charles; Erable, Benjamin

doi:10.1016/j.jenvman.2020.111859

Cited by 23 publications

(6 citation statements)

References 69 publications

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“…In addition, it also provides a safety margin in case of unwanted alkalinization (Food Agriculture Organization of the United Nations, 2014 ). Although certain pH robustness is known for denitrifying microbiomes reporting microbial denitrification at up to pH 11–12 (Albina et al, 2021 ), this is, to our knowledge, not reported for bioelectrochemical denitrification. This broad pH range further supports the biotechnological potential of bioelectrochemical denitrification and extends its fields of application to moderate alkaline environments.…”

Section: Resultsmentioning

confidence: 59%

Electrochemical and Microbial Dissection of Electrified Biotrickling Filters

Korth

Pous²,

Honig³

et al. 2022

Front. Microbiol.

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Electrified biotrickling filters represent sustainable microbial electrochemical technology for treating organic carbon-deficient ammonium-contaminated waters. However, information on the microbiome of the conductive granule bed cathode remains inexistent. For uncovering this black box and for identifying key process parameters, minimally invasive sampling units were introduced, allowing for the extraction of granules from different reactor layers during reactor operation. Sampled granules were analyzed using cyclic voltammetry and molecular biological tools. Two main redox sites [−288 ± 18 mV and −206 ± 21 mV vs. standard hydrogen electrode (SHE)] related to bioelectrochemical denitrification were identified, exhibiting high activity in a broad pH range (pH 6–10). A genome-centric analysis revealed a complex nitrogen food web and the presence of typical denitrifiers like Pseudomonas nitroreducens and Paracoccus versutus with none of these species being identified as electroactive microorganism so far. These are the first results to provide insights into microbial structure-function relationships within electrified biotrickling filters and underline the robustness and application potential of bioelectrochemical denitrification for environmental remediation.

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Section: Resultsmentioning

confidence: 59%

Electrochemical and Microbial Dissection of Electrified Biotrickling Filters

Korth

Pous²,

Honig³

et al. 2022

Front. Microbiol.

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“…IET Associated Syntrophic Coupling between Syntrophomonas and Denitrified Denitrifiers of Rhodoclyclaceae. Despite such coupling of acetate oxidation and denitrification observed in several digestion reactors, 81,82 this study provided the first convincing evidence for such coupling in a TAD system and revealed its association with conductive material-mediated interspecies electron transfer (MIET). Therefore, IET capabilities of Rhodoclyclaceae species and the ecological implication of its coupling with syntrophic acetate oxidation in AD systems deserve further investigations.…”

Section: Spatial Association Co-occurrence Network Based On Micron-sc...mentioning

confidence: 81%

Electric-Inducive Microbial Interactions in a Thermophilic Anaerobic Digester Revealed by High-Throughput Sequencing of Micron-Scale Single Flocs

Zhao

Chen

Zhang

et al. 2023

Environ. Sci. Technol.

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Although conductive materials have been shown to improve efficiency in anaerobic digestion (AD) by modifying microbial interactions, the interacting network under thermophilic conditions has not been examined. To identify the true taxon–taxon associations within thermophilic anaerobic digestion (TAD) microbiome and reveal the influence of carbon cloth (CC) addition, we sampled micron-scale single flocs (40–70 μm) randomly isolated from lab-scale thermophilic digesters. Results revealed that CC addition not only significantly boosted methane yield by 25.3% but also increased the spatial heterogeneity of the community in the sludge medium. After CC addition, an evident translocation of Pseudomonas from the medium to the biofilm was observed, showing their remarkable capacity for biofilm formation. Additionally, Clostridium and Thermotogaceae tightly aggregated and steadily co-occurred in the medium and biofilm of the TAD microbiome, which might be associated with their unique extracellular sugar metabolizing style. Finally, CC induced syntrophic interaction between Syntrophomonas and denitrifiers of Rhodocyclaceae. The upregulated respiration-associated electron transferring genes (Cyst-c, complex III) on the cellular membranes of these collaborating partners indicated a potential coupling of the denitrification pathway with syntrophic acetate oxidation via direct interspecies electron transfer (DIET). These findings provide an insight into how conductive materials promote thermophilic digestion performance and open the path for improved community monitoring of biotreatment systems.

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“…Studies have shown that maintaining adequate alkalinity concentrations is essential to maintain nitrification [ 19 ], and at pH near neutral and alkaline variations in the range of 6.5–9.0, nitrite nitrogen accumulation rises gradually with increasing pH, with pH = 9.0 being more favorable for the accumulation of nitrite nitrogen in denitrification [ 20 ]. In the presence of NO, pH has a strong influence on the kinetics and mechanism of abiotic transformation, and low pH conditions tend to easily break S-N and N-C bonds and promote nitrification and nitrosation reactions [ 21 ].…”

Section: Introductionmentioning

confidence: 99%

Changes in pH and Nitrite Nitrogen Induces an Imbalance in the Oxidative Defenses of the Spotted Babylon (Babylonia areolata)

Ding,

Yang,

et al. 2023

Antioxidants

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In order to reveal the acute toxicity and physiological changes of the spotted babylon (Babylonia areolata) in response to environmental manipulation, the spotted babylon was exposed to three pH levels (7.0, 8.0 and 9.0) of seawater and four concentrations of nitrite nitrogen (0.02, 2.7, 13.5 and 27 mg/L). The activities of six immunoenzymes, superoxide dismutase (SOD), glutathione peroxidase (GSH-PX), catalase (CAT), acid phosphatase (ACP), alkaline phosphatase (AKP) and peroxidase (POD), were measured. The levels of pH and nitrite nitrogen concentrations significantly impacted immunoenzyme activity over time. After the acute stress of pH and nitrite nitrogen, the spotted babylon appeared to be unresponsive to external stimuli, exhibited decreased vigor, slowly climbed the wall, sank to the tank and could not stand upright. As time elapsed, with the extension of time, the spotted babylon showed a trend of increasing and then decreasing ACP, AKP, CAT and SOD activities in order to adapt to the mutated environment and improve its immunity. In contrast, POD and GSH-PX activities showed a decrease followed by an increase with time. This study explored the tolerance range of the spotted babylon to pH, nitrite nitrogen, and time, proving that external stimuli activate the body’s immune response. The body’s immune function has a specific range of adaptation to the environment over time. Once the body’s immune system was insufficient to adapt to this range, the immune system collapsed and the snail gradually died off. This study has discovered the suitable pH and nitrite nitrogen ranges for the culture of the spotted babylon, and provides useful information on the response of the snail’s immune system.

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Nitrate and nitrite bacterial reduction at alkaline pH and high nitrate concentrations, comparison of acetate versus dihydrogen as electron donors

Abstract: Nitrate and nitrite bacterial reduction at alkaline pH and high nitrate concentrations, comparison of acetate versus dihydrogen as electron donors. (2021) Journal of Environmental Management, 280. 111859.

Cited by 23 publications

References 69 publications

Electrochemical and Microbial Dissection of Electrified Biotrickling Filters

Electrochemical and Microbial Dissection of Electrified Biotrickling Filters

Electric-Inducive Microbial Interactions in a Thermophilic Anaerobic Digester Revealed by High-Throughput Sequencing of Micron-Scale Single Flocs

Changes in pH and Nitrite Nitrogen Induces an Imbalance in the Oxidative Defenses of the Spotted Babylon (Babylonia areolata)

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