Nitrate reductase activity in macroalgae and its vertical distribution in macroalgal epiphytes of seagrasses

Young, Erica B.; Lavery, Paul S.; Elven, B. van; Dring, M. J.; Berges, John A.

doi:10.3354/meps288103

Cited by 31 publications

(11 citation statements)

References 39 publications

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“…n.d. = not detected. (Thomas and Harrison, 1985;Young et al, 2005). Another, more likely, possibility is that NO 3 À uptake was limited by Fe because NO 3 À utilization depends on Fe-containing enzymes and requires high cellular Fe quotes (Viaroli et al, 2005).…”

Section: Nutrient Uptake Rates and Total Organic Carbon Releasementioning

confidence: 94%

Responses of bloom forming and non-bloom forming macroalgae to nutrient enrichment in Hawai‘i, USA

Dailer

Smith

2012

Harmful Algae

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Section: Nutrient Uptake Rates and Total Organic Carbon Releasementioning

confidence: 94%

Responses of bloom forming and non-bloom forming macroalgae to nutrient enrichment in Hawai‘i, USA

Dailer

Smith

2012

Harmful Algae

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“…Frozen tissue samples (∼0.15 g) were placed in a mortar and pestle with ∼10 mL of liquid N and ground into a fine powder immediately after the liquid N had evaporated. In vitro extraction and measurements of nitrate reductase activity were made as described by Hurd et al (1995) and as modified by Young et al (2005). Each extract was measured in triplicate with one time‐zero control.…”

Section: Methodsmentioning

confidence: 99%

RESPONSE OF LAMINARIA SACCHARINA (PHAEOPHYTA) GROWTH AND PHOTOSYNTHESIS TO SIMULTANEOUS ULTRAVIOLET RADIATION AND NITROGEN LIMITATION¹

Davison¹,

Jordan

Fegley

et al. 2007

Journal of Phycology

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The brown macroalga Laminaria saccharina (L.) J. V. Lamour. was grown in large outdoor tanks at 50% ambient solar radiation for 3–4 weeks in July and August of 2000, 2001, and 2002, in either ambient or nitrogen (N)–enriched seawater and in either ambient light [PAR + ultraviolet radiation (UVR)] or ambient light minus UVR. Growth, N‐content, photosynthetic pigments, and RUBISCO content increased in N‐enriched seawater, indicating N‐limitation. UVR inhibited growth, but this inhibition was ameliorated by N‐enrichment. The response of growth to UVR could not be explained by changes in respiration and photosynthesis. Gross light‐saturated photosynthesis (Pmax) remained unaffected by UVR but was significantly higher under N‐enrichment, as was dark respiration (Rd). UVR had no effect on pigments or N content. However, RUBISCO contents were low in the presence of UVR, reflecting the overall change in soluble cellular protein. Overall, our data indicate that the response to UVR in L. saccharina depends on other environmental factors, such as N, and these effects need to be considered when evaluating the response of macroalgae to increased UVR.

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“…Therefore, full growth potential is unlikely to be achieved in this treatment pond, or any pond where ammonium has not been oxidised to nitrate. The explanation for this antagonistic result is that ammonium can inhibit nitrate uptake by inactivating or slowing the synthesis of nitrate reductase (Berges et al 1995, Young et al 2005), even at low concentrations (< 5 µM) (Thomas & Harrison 1987). Enhancing the oxidation of ammonium to nitrate by denitrifying bacteria using biofilters could be an important strategy to complement existing conversions in order to increase the nitrate to total ammonium nitrogen ratio >1:3, as even this ratio was detrimental in our study.…”

Section: Discussionmentioning

confidence: 89%

Seasonal growth dynamics and resilience of the green tide alga Cladophora coelothrix in high-nutrient tropical aquaculture

Silva¹,

Nys²,

Paul³

2012

Aquacult. Environ. Interact.

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Two distinct climatic seasons in the tropics generate extreme environmental fluctuations in land-based aquaculture and corresponding challenges for integrated aquaculture. Here we assessed the influence of environmental and culture variables on productivity of Cladophora coelothrix at multiple scales, firstly in an operational settlement pond and subsequently in laboratory and flow-through mesocosm experiments. Classification and regression tree (CART) analyses were used to partition the influence of key variables on growth. In situ productivity correlated primarily with nitrogen concentration, position in the pond and stocking density, with a lesser influence of salinity, temperature and the ratio of nitrate-nitrogen (NO 3 − -N) and total ammonium nitrogen (TAN). We subsequently evaluated the resilience of C. coelothrix in laboratory and mesocosm environments by manipulating salinity, temperature and nutrients across a broader range of values derived from a year-long survey of the settlement pond. Here temperature had the greatest influence on growth, followed by nitrogen concentration and salinity. Growth rates of C. coelothrix were high, irrespective of season, except at the extremes of each season, for example, at the lowest annual temperature and with the combination of high temperature and low salinity. Growth was also limited by ~20% when NO 3 − -N and TAN were available simultaneously. The use of CART analyses for both in situ and controlled environment data provided a formal dissection of the relative contribution of each environmental variable to growth, demonstrating the year-round potential for bioremediation and identifying the critical combinations of environmental variables for management of this system.

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Nitrate reductase activity in macroalgae and its vertical distribution in macroalgal epiphytes of seagrasses

Cited by 31 publications

References 39 publications

Responses of bloom forming and non-bloom forming macroalgae to nutrient enrichment in Hawai‘i, USA

Responses of bloom forming and non-bloom forming macroalgae to nutrient enrichment in Hawai‘i, USA

RESPONSE OF LAMINARIA SACCHARINA (PHAEOPHYTA) GROWTH AND PHOTOSYNTHESIS TO SIMULTANEOUS ULTRAVIOLET RADIATION AND NITROGEN LIMITATION¹

Seasonal growth dynamics and resilience of the green tide alga Cladophora coelothrix in high-nutrient tropical aquaculture

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Nitrate reductase activity in macroalgae and its vertical distribution in macroalgal epiphytes of seagrasses

Cited by 31 publications

References 39 publications

Responses of bloom forming and non-bloom forming macroalgae to nutrient enrichment in Hawai‘i, USA

Responses of bloom forming and non-bloom forming macroalgae to nutrient enrichment in Hawai‘i, USA

RESPONSE OF LAMINARIA SACCHARINA (PHAEOPHYTA) GROWTH AND PHOTOSYNTHESIS TO SIMULTANEOUS ULTRAVIOLET RADIATION AND NITROGEN LIMITATION1

Seasonal growth dynamics and resilience of the green tide alga Cladophora coelothrix in high-nutrient tropical aquaculture

Contact Info

Product

Resources

About

RESPONSE OF LAMINARIA SACCHARINA (PHAEOPHYTA) GROWTH AND PHOTOSYNTHESIS TO SIMULTANEOUS ULTRAVIOLET RADIATION AND NITROGEN LIMITATION¹