Nitrate reductase from Achromobacter fischeri. Purification and properties: Function of flavines and cytochrome

Sadana, J.C.; McElroy, W. D.

doi:10.1016/0003-9861(57)90242-4

Cited by 78 publications

(19 citation statements)

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“…Protein was determined by the Folin method (Lowry, Rosebrough, Farr & Randall, 1951) and the total bacterialnitrogen by micro-Kjeldahl. The 15N technique used was that described by San Pietro (1957) and Simms & Cocking (1958 These results suggest that this bacterium, which has been used under the name Achrornobacter Jischeri by Sadana & McElroy (1957), is very similar to Photobacterium sepia described by Spencer (1955). The genus Photobacterium is a member of the Pseudomonadaceae and is no longer referred to as Achromobacter (Bergey's ManuaE, 1957).…”

Section: Organismmentioning

confidence: 60%

“…were centrifuged at 25,400 g for 20 min. a t 4' as described by Sadana & McElroy (1957). The sediment was re-extracted with water (1 g. wet wt./15 ml.)…”

Section: Organismmentioning

confidence: 99%

“…Reduced benzylviologen, prepared by the palladized asbestos-hydrogen method, did not function as a hydrogen donor for nitrate reductase in cell-free extracts. The dye reduced with sodium dithionite was, however, a suitable donor for the extracted enzyme (Sadana & McElroy, 1957). The specific activity of the enzyme is defined as pm-mole NO2-formed/lO min./mg./bacterial total-N. (v/v) NO in N, until damp litmus paper turned red when held in the exit gas stream from the side-arm stopper.…”

Section: Organismmentioning

confidence: 99%

“…A nitrate reductase characterized in Achromobacter fischeri by Sadana & McElroy (1957) was shown to be induced by its substrate only. They claimed that organisms deficient in iron had decreased enzyme activity but that a deficiency of other micronutrients, including molybdenum, was without effect.…”

Section: Introductionmentioning

confidence: 99%

“…Lindeberg, Lode & Somme (1963) found that nitrate reductase in Achromobacter species decreased when the oxygen pressure in the medium was increased and in this respect it resembled the dissimilatory enzyme from other bacteria. We have re-examined the cultures used by Sadana & McElroy (1957) and find that the bacterium used by them is in fact Photobacterium sepia which can be readily trained to grow with nitrate as sole nitrogen source. By using reduced benzylviologen as hydrogen donor we have found that the enzyme is dependent on both molybdenum and iron for its activity (Nicholas, Redmond & Wright, 1963).…”

Section: Introductionmentioning

confidence: 99%

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Effects of Cultural Conditions on Nitrate Reductase in Photobacterium sepia

Nicholas

Redmond

Wright

1964

Journal of General Microbiology

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SUMMARYThe effect of cultural conditions on the growth and nitrate reductase activity of a bacterium, now identified as Photobacterium sepia, were examined. The bacterium grew well with ammonium chloride, ammonium nitrate or potassium nitrate as sole nitrogen source. Nitrate reductase activity was markedly decreased in organisms deficient in molybdenum or iron and in organisms grown a t high oxygen pressures. The P. sepia enzyme is thus similar to nitrate reductases from other bacteria. The P. sepia enzyme is constitutive; it is present in organisms grown with ammonium chloride in the absence of nitrate; the ammonium radical did not depress enzyme activity. Under certain conditions (NH,NO, medium, 10 yo, v/v, 0, in N,) molybdenum (10 pg./l.) depressed the nitrate reductase activity, but this effect was annulled by repeated subcultivation in molybdenumdeficient medium. Vanadium and especially tungsten, also inhibited the enzyme. The uptake of nitric oxide was decreased in organisms depleted of iron but was increased in organisms deficient in molybdenum. The behaviour of nitrate reductase preparations during fractionation whether from P. sepia grown with KNO, or with NH&1 suggests that the enzyme is the same from both sources.

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Section: Organismmentioning

confidence: 60%

“…were centrifuged at 25,400 g for 20 min. a t 4' as described by Sadana & McElroy (1957). The sediment was re-extracted with water (1 g. wet wt./15 ml.)…”

Section: Organismmentioning

confidence: 99%

Section: Organismmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%