Nitrate Reductase from the Marine Diatom Skeletonema costatum (Biochemical and Immunological Characterization)

Gao, Yu; Smith, G. Jason; Alberte, Randall S.

doi:10.1104/pp.103.4.1437

Cited by 45 publications

(55 citation statements)

References 30 publications

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“…Also, the interaction of NR with C-metabolism is thought to underlie partitioning of cellular energy into growth rather than to storage. Diatom NR proteins were first purified and characterized in Thalassiosira pseudonana and Skeletonema costatum (Amy and Garrett, 1974;Gao et al, 1993), and the gene was first isolated and characterized in the diatom Phaeodactylum tricornutum (Allen et al, 2005). Plant NR structure was first described in maize (Zea mays; Lu et al, 1992).…”

Section: Introductionmentioning

confidence: 99%

Nitrate Reductase Knockout Uncouples Nitrate Transport from Nitrate Assimilation and Drives Repartitioning of Carbon Flux in a Model Pennate Diatom

et al. 2017

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Section: Introductionmentioning

confidence: 99%

Nitrate Reductase Knockout Uncouples Nitrate Transport from Nitrate Assimilation and Drives Repartitioning of Carbon Flux in a Model Pennate Diatom

et al. 2017

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“…In these three species we also examined the thermal response of nitrate reductase (NR), a key enzyme of nitrate assimilation. It has been demonstrated that the thermal stability of this enzyme in marine phytoplankton may have ecologically significant influences on population structure and geographic distributions, as well as on the temporal dynamics of bloom formations (Gao et al ., 1993(Gao et al ., , 2000; it is relevant that in Skeletonema costatum and other marine phytoplankton species, nitrate reductase exhibits temperature optima ranging from 10 to 20 ° C, the typical growth temperatures of these algae (Gao et al ., 1993). Our present results have shown that NADH : NR of K. antarctica and ' C .'…”

Section: Introductionmentioning

confidence: 99%

Temperature responses of growth, photosynthesis, respiration and NADH: nitrate reductase in cryophilic and mesophilic algae

et al. 2004

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Summary• Temperature effects on growth, photosynthesis, respiration and nitrate reductase (NR) were studied in the cryophilic algae Koliella antarctica and ' Chlorella ' saccharophila , and in the mesophilic Chlorella sorokiniana .• Growth rate was measured as increase in optical density. Photosynthesis at saturating light and respiration in darkness were measured as O 2 exchange. NADH : NR was assayed in crude extracts.• The two cryophilic algae grew below 15 ° C, and C. sorokiniana above 20 ° C. Photosynthetic and respiration rates of K. antarctica and ' C .' saccharophila were elevated at 5 ° C, and peaked at 30 ° C. Arrhenius plots from 5 to 25 ° C were linear in K. antarctica , whereas in ' C .' saccharophila and C. sorokiniana they exhibited breaks at 15 and 20 ° C, respectively. Values for activation energy ( E a ) and the factor by which the rate increases with raising the temperature 10 ° C ( Q 10 ) differed. Nitrate reductase had its optimum at 25 ° C in cryophilic algae and at 35 ° C in C. sorokiniana .• We conclude that growth of cryophilic algae at low temperature is favoured by elevated photosynthesis and respiration rates, but that it could be limited by a high respiration : photosynthesis ratio.

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“…Nitrate is taken up into the diatom cell, where it is first reduced to nitrite by a cytosolic NADH-dependent nitrate reductase (NR; Gao et al, 1993;Berges and Harrison, 1995;Allen et al, 2005). Nitrite is then transported into the chloroplast and further reduced to ammonium by a cyanobacterium-like ferredoxindependent nitrite reductase (Fd-NiR; Milligan and Harrison, 2000;Bowler et al, 2010).…”

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confidence: 99%

The Response of Diatom Central Carbon Metabolism to Nitrogen Starvation Is Different from That of Green Algae and Higher Plants

Hockin

Möck²,

Mulholland³

et al. 2011

Plant Physiology

334

304

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The availability of nitrogen varies greatly in the ocean and limits primary productivity over large areas. Diatoms, a group of phytoplankton that are responsible for about 20% of global carbon fixation, respond rapidly to influxes of nitrate and are highly successful in upwelling regions. Although recent diatom genome projects have highlighted clues to the success of this group, very little is known about their adaptive response to changing environmental conditions. Here, we compare the proteome of the marine diatom Thalassiosira pseudonana (CCMP 1335) at the onset of nitrogen starvation with that of nitrogen-replete cells using two-dimensional gel electrophoresis. In total, 3,310 protein spots were distinguishable, and we identified 42 proteins increasing and 23 decreasing in abundance (greater than 1.5-fold change; P , 0.005). Proteins involved in the metabolism of nitrogen, amino acids, proteins, and carbohydrates, photosynthesis, and chlorophyll biosynthesis were represented. Comparison of our proteomics data with the transcriptome response of this species under similar growth conditions showed good correlation and provided insight into different levels of response. The T. pseudonana response to nitrogen starvation was also compared with that of the higher plant Arabidopsis (Arabidopsis thaliana), the green alga Chlamydomonas reinhardtii, and the cyanobacterium Prochlorococcus marinus. We have found that the response of diatom carbon metabolism to nitrogen starvation is different from that of other photosynthetic eukaryotes and bears closer resemblance to the response of cyanobacteria.

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Nitrate Reductase from the Marine Diatom Skeletonema costatum (Biochemical and Immunological Characterization)

Cited by 45 publications

References 30 publications

Nitrate Reductase Knockout Uncouples Nitrate Transport from Nitrate Assimilation and Drives Repartitioning of Carbon Flux in a Model Pennate Diatom

Nitrate Reductase Knockout Uncouples Nitrate Transport from Nitrate Assimilation and Drives Repartitioning of Carbon Flux in a Model Pennate Diatom

Temperature responses of growth, photosynthesis, respiration and NADH: nitrate reductase in cryophilic and mesophilic algae

The Response of Diatom Central Carbon Metabolism to Nitrogen Starvation Is Different from That of Green Algae and Higher Plants

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