Nitrate removal performances of a new aerobic denitrifier, Acinetobacter haemolyticus ZYL, isolated from domestic wastewater

Wang, Ying; Zou, Yu-Lan; Hu, Chen; Lv, Yongkang

doi:10.1007/s00449-020-02451-0

Cited by 31 publications

(6 citation statements)

References 39 publications

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“…Moreover, NO 3 − must be converted into available nitrogen sources via assimilative NO 3 − reduction for cell synthesis [33] . However, such a process may occur when NH 4 + is exhausted, because strain R-1 prefers NH 4 + over NO 3 − as a nitrogen source, similar to P. stutzeri T13, Rhodococcus erythropolis Y10, Acinetobacter haemolyticus ZYL, and Pseudomonas aeruginosa P-1 [6,16,33,34] .…”

Section: Resultsmentioning

confidence: 99%

Efficient nitrogen removal via simultaneous ammonium assimilation and heterotrophic denitrification of Paracoccus denitrificans R-1 under aerobic and anaerobic conditions

Hong,

Sun,

et al. 2024

Preprint

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Although multiple microorganisms can remove ammonium and nitrate simultaneously, their metabolic mechanisms are not well understood. Strain R-1, isolated from the activated sludge of a sewage treatment plant, was identified as Paracoccus denitrificans, and was found to efficiently remove ammonium and nitrate under anaerobic and aerobic conditions. The maximal NH4+ removal rate (RR = 9.94 mg·L− 1·h− 1) was significantly higher under aerobic conditions than under anaerobic conditions (RR = 2.91 mg·L− 1·h− 1). Analysis of the nitrogen balance and isotope tracers indicated that NH4+ was consumed through assimilation, but not nitrification. The maximal NO3− RR of strain R-1 was 18.05 and 19.76 mg·L− 1·h− 1 under aerobic and anaerobic conditions, respectively, and NO3− reduction was able to support the growth of R-1 under anaerobic conditions. The stoichiometric consumption ratios of acetate and lactate to nitrate were 0.902 and 0.691, respectively. The 15NO3− isotopic tracer experiment demonstrated that NO3− was reduced to N2 by aerobic and anaerobic denitrification. These results indicated that the NO3− reduction by strain R-1 was a respiratory process coupled with the oxidation of electron donors. Genomic analysis showed that strain R-1 contained complete genes for the nitrogen metabolism pathways of ammonium assimilation and denitrification, but not for nitrification, which is consistent with the physiological process of inorganic nitrogen metabolism in strain R-1. Moreover, we found that ammonium assimilation and nitrate denitrification effectively promoted each other. Our findings demonstrate that the mechanism of the simultaneous removal of NH4+ and NO3− by strain R-1 involves ammonium assimilation and denitrification under aerobic and anaerobic conditions. These findings provide new insights into microbial nitrogen transformation and facilitate the simultaneous removal of NH4+ and NO3− in a single reaction system.

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Section: Resultsmentioning

confidence: 99%

Efficient nitrogen removal via simultaneous ammonium assimilation and heterotrophic denitrification of Paracoccus denitrificans R-1 under aerobic and anaerobic conditions

Hong,

Sun,

et al. 2024

Preprint

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“…where C 0 and Ct represent the initial and corresponding NO 2 − -N concentrations (mg/L) at time t, respectively [18]. The concentration units for C 0 and C t are mg/L.…”

Section: Amplification Of Denitrification Functional Genesmentioning

confidence: 99%

Nitrite Degradation by a Novel Marine Bacterial Strain Pseudomonas aeruginosa DM6: Characterization and Metabolic Pathway Analysis

Chen,

Yu,

Zhan

et al. 2024

Water

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High concentrations of nitrite in marine aquaculture wastewater not only pose a threat to the survival and immune systems of aquatic organisms but also contribute to eutrophication, thereby impacting the balance of coastal ecosystems. Compared to traditional physical and chemical methods, utilizing microorganism-mediated biological denitrification is a cost-effective and efficient solution. However, the osmotic pressure changes and salt-induced enzyme precipitation in high-salinity seawater aquaculture environments may inhibit the growth and metabolism of freshwater bacterial strains, making it more suitable to select salt-tolerant marine microorganisms for treating nitrite in marine aquaculture wastewater. In this study, a salt-tolerant nitrite-degrading bacterium, designated as DM6, was isolated from the seawater (salinity of 25–30‰) of Portunus trituberculatus cultivation. The molecular identification of strain DM6 was conducted using 16S rRNA gene sequencing technology. The impacts of various environmental factors on the nitrite degradation performance of strain DM6 were investigated through single-factor and orthogonal experiments, with the selected conditions considered to be the key factors affecting the denitrification efficiency of microorganisms in actual wastewater treatment. PCR amplification of key genes involved in the nitrite metabolism pathway of strain DM6 was conducted, including denitrification pathway-related genes narG, narH, narI, nirS, and norB, as well as assimilation pathway-related genes nasC, nasD, nasE, glnA, gltB, gltD, gdhB, and gdhA. The findings indicated that strain DM6 is classified as Pseudomonas aeruginosa and exhibits efficient nitrite degradation even under a salinity of 35‰. The optimal nitrite degradation efficiency of DM6 was observed when using sodium citrate as the carbon source, a C/N ratio of 20, a salinity of 13‰, pH 8.0, and a temperature of 35 °C. Under these conditions, DM6 could completely degrade an initial nitrite concentration of 156.33 ± 1.17 mg/L within 36 h. Additionally, the successful amplification of key genes involved in the nitrite denitrification and assimilation pathways suggests that strain DM6 may possess both denitrification and assimilation pathways for nitrite degradation simultaneously. Compared to freshwater strains, strain DM6 demonstrates higher salt tolerance and exhibits strong nitrite degradation capability even at high concentrations. However, it may be more suitable for application in the treatment of wastewater from marine aquaculture systems during summer, high-temperature, or moderately alkaline conditions.

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“…The reported partial napA aa sequence (QCY54495.1, from Acinetobacter hemolyticus) was used for blast in the GenBank database to check the distribution of homologous genes in Acinetobacter nitrite reductase [27]. NP_249210.1 nitrite reductase [Pseudomonas aeruginosa PAO1] was also used to blast the protein database to identify homologous proteins for the Acinetobacter genus.…”

Section: Genetic Investigation Of the Nitrogen Removal Potential In S...mentioning

confidence: 99%

Characterization of the Nitrogen Removal Potential of Two Newly Isolated Acinetobacter Strains under Low Temperature

Zhong,

Xia

2023

Water

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Excess nitrogen and phosphorus in the water causes several ecological problems for nutrients. Biological nitrogen removal is an economical and efficient way to prevent excessive nitrogen in the environment. For most areas of China, temperatures are usually lower than 20 °C except during the summertime. It is necessary to discover microbes that can efficiently remove nitrogen at low temperatures. In this study, two Acinetobacter strains were isolated from a sample in a wastewater tank in Taizhou for their capabilities to remove NO3−–N and NO2−–N at 15 °C. Heterotrophic nitrification experiments showed that both strains could efficiently remove nitrogen from the culture medium. The maximum removal rates of NH4+–N were 3.15 mg/L·h and 4.74 mg/L·h for heterotrophic nitrification by the strains F and H, respectively. Strain H grew faster and removed both nitrite and nitrate more efficiently than strain F. Genome sequencing showed that strains F and H could be classified into Acinetobacter johnsonii and Acinetobacter bereziniae, respectively. NO2−–N (100 mg/L) was completely removed in 3 days by strain H. The maximum NO3−–N removal rate was 3.53 mg/L·h for strain F. When strain H was cultured in a broth with 200 mg/L NO3−–N, 97.46% of NH4+–N (200 mg/L) was removed in 5 days, and the maximum NH4+–N removal rate was 4.04 mg/L·h. Genomic sequence analysis showed that both the strains lacked genes involved in the denitrification pathway that transforms NO3− into N2. This implies that nitrate or nitrite is removed through the nitrogen assimilation pathway. Genes responsible for nitrate assimilation are clustered together with molybdopterin cofactor biosynthesis genes. Strain H contains fewer resistance genes and transfer elements. All the above data demonstrate that strain H is a promising candidate for nitrogen removal at lower temperatures. But there is still a lot to be done to systematically evaluate the potential of A. bereziniae strain H in treating wastewater at a pilot scale. These include the long-term performance, environmental tolerance, and nitrogen removal efficiency in wastewater. And the application of these Acinetobacter strains in diverse wastewater treatment settings might require careful optimization and real-time monitoring.

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Nitrate removal performances of a new aerobic denitrifier, Acinetobacter haemolyticus ZYL, isolated from domestic wastewater

Cited by 31 publications

References 39 publications

Efficient nitrogen removal via simultaneous ammonium assimilation and heterotrophic denitrification of Paracoccus denitrificans R-1 under aerobic and anaerobic conditions

Efficient nitrogen removal via simultaneous ammonium assimilation and heterotrophic denitrification of Paracoccus denitrificans R-1 under aerobic and anaerobic conditions

Nitrite Degradation by a Novel Marine Bacterial Strain Pseudomonas aeruginosa DM6: Characterization and Metabolic Pathway Analysis

Characterization of the Nitrogen Removal Potential of Two Newly Isolated Acinetobacter Strains under Low Temperature

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