Nitrative DNA damage in lung epithelial cells exposed to indium nanoparticles and indium ions

Sharif, Ahmed; Kobayashi, Hatasu; Afroz, Tania; Ma, Ning; Oikawa, Shinji; Kawanishi, Shosuke; Murata, Mariko; Hiraku, Yusuke

doi:10.1038/s41598-020-67488-3

Cited by 9 publications

(6 citation statements)

References 42 publications

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“…HMGB1 is a nuclear protein that is released from dead cells [ 39 ]. We have previously shown that indium compounds induced inflammation-mediated DNA damage in lung epithelial cells via the HMGB1 pathway [ 40 ]. In addition, by binding to toll-like receptor (TLR) 4, HMGB1 activates myeloid differentiation (MyD) 88 and NF-κB essential modulator (NEMO), and activates inflammatory cytokines such as IL-6 and TNF-α via nuclear factor-kappa B (NF-κB) [ 16 , 17 , 18 , 41 , 42 , 43 , 44 ].…”

Section: Discussionmentioning

confidence: 99%

Glycyrrhizin Attenuates Carcinogenesis by Inhibiting the Inflammatory Response in a Murine Model of Colorectal Cancer

Wang

Hiramoto

et al. 2021

IJMS

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Glycyrrhizin (GL), an important active ingredient of licorice root, which weakens the proinflammatory effects of high-mobility group box 1 (HMGB1) by blocking HMGB1 signaling. In this study, we investigated whether GL could suppress inflammation and carcinogenesis in an azoxymethane (AOM)/dextran sodium sulfate (DSS)-induced murine model of colorectal cancer. ICR mice were divided into four groups (n = 5, each)—control group, GL group, colon cancer (CC) group, and GL-treated CC (CC + GL) group, and sacrificed after 20 weeks. Plasma levels of interleukin (IL)-6 and tumor necrosis factor (TNF)-α were measured using an enzyme-linked immunosorbent assay. The colonic tissue samples were immunohistochemically stained with DNA damage markers (8-nitroguanine and 8-oxo-7,8-dihydro-2′-deoxy-guanosine), inflammatory markers (COX-2 and HMGB1), and stem cell markers (YAP1 and SOX9). The average number of colonic tumors and the levels of IL-6 and TNF-α in the CC + GL group were significantly lower than those in the CC group. The levels of all inflammatory and cancer markers were significantly reduced in the CC + GL group. These results suggest that GL inhibits the inflammatory response by binding HMGB1, thereby inhibiting DNA damage and cancer stem cell proliferation and dedifferentiation. In conclusion, GL significantly attenuates the pathogenesis of AOM/DSS-induced colorectal cancer by inhibiting HMGB1-TLR4-NF-κB signaling.

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Section: Discussionmentioning

confidence: 99%

Glycyrrhizin Attenuates Carcinogenesis by Inhibiting the Inflammatory Response in a Murine Model of Colorectal Cancer

Wang

Hiramoto

et al. 2021

IJMS

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“…Genotoxicity of indium, in distinct speciation as compared to its speciation in present study, has been reported in the literature. Not only indium oxide and indium tin oxide particles and nanoparticles but also indium chloride have been reported to be genotoxic via the comet assay, micronucleus assay, and measurement of 8-nitroguanine DNA lesion (Tabei et al, 2018 ; Ahmed et al, 2020 ; Tsai et al, 2020 ). While Tsai et al ( 2020 ) attribute indium-related genotoxicity to oxidative stress and mitochondrial dysfunction, Ahmed et al ( 2020 ) rather attribute it to inflammation that triggers 8-nitroguanine formation in the DNA of exposed cells.…”

Section: Discussionmentioning

confidence: 99%

“…Not only indium oxide and indium tin oxide particles and nanoparticles but also indium chloride have been reported to be genotoxic via the comet assay, micronucleus assay, and measurement of 8-nitroguanine DNA lesion (Tabei et al, 2018 ; Ahmed et al, 2020 ; Tsai et al, 2020 ). While Tsai et al ( 2020 ) attribute indium-related genotoxicity to oxidative stress and mitochondrial dysfunction, Ahmed et al ( 2020 ) rather attribute it to inflammation that triggers 8-nitroguanine formation in the DNA of exposed cells. Tabei et al ( 2018 ) demonstrate that indium genotoxicity is not linked with oxidative stress but that it can be related to intracellular degradation of the indium-containing particles, which leads to accumulation of In(III) ions in the nuclei of exposed cells and direct interaction of indium with the DNA.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of the Dermal Toxicity of InZnP Quantum Dots Before and After Accelerated Weathering: Toward a Safer-By-Design Strategy

et al. 2021

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Quantum dots (QDs) are colloidal fluorescent semiconductor nanocrystals with exceptional optical properties. Their widespread use, particularly in light-emitting diodes (LEDs), displays, and photovoltaics, is questioning their potential toxicity. The most widely used QDs are CdSe and CdTe QDs, but due to the toxicity of cadmium (Cd), their use in electrical and electronic equipment is now restricted in the European Union through the Restriction of hazardous substances in electrical and electronic equipment (RoHS) directive. This has prompted the development of safer alternatives to Cd-based QDs; among them, InP QDs are the most promising ones. We recently developed RoHS-compliant QDs with an alloyed core composed of InZnP coated with a Zn(Se,S) gradient shell, which was further coated with an additional ZnS shell to protect the QDs from oxidative surface degradation. In this study, the toxicity of single-shelled InZnP/Zn(Se,S) core/gradient shell and of double-shelled InZnP/Zn(Se,S)/ZnS core/shell/shell QDs was evaluated both in their pristine form and after aging in a climatic chamber, mimicking a realistic environmental weathering. We show that both pristine and aged QDs, whatever their composition, accumulate in the cytoplasm of human primary keratinocytes where they form agglomerates at the vicinity of the nucleus. Pristine QDs do not show overt toxicity to cells, while aged QDs show cytotoxicity and genotoxicity and significantly modulate the mRNA expression of proteins involved in zinc homeostasis, cell redox response, and inflammation. While the three aged QDs show similar toxicity, the toxicity of pristine gradient-shell QD is higher than that of pristine double-shell QD, confirming that adding a second shell is a promising safer-by-design strategy. Taken together, these results suggest that end-of-life degradation products from InP-based QDs are detrimental to skin cells in case of accidental exposure and that the mechanisms driving this effect are oxidative stress, inflammation, and disturbance of cell metal homeostasis, particularly Zn homeostasis. Further efforts to promote safer-by-design formulations of QDs, for instance by reducing the In and Zn content and/or implementing a more robust outer shell, are therefore warranted.

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“…The mechanism of carcinogenicity of ITO has been investigated in laboratory animals and it was found that indium compounds including nanoparticles of In 2 O 3 , ITO, and indium chloride (InCl 3 ) significantly increased 8-Nitroguanine (8-nitroG; a mutagenic DNA lesion) formation and stimulated inflammation-mediated DNA damage in lung epithelial cells via the HMGB1-RAGE-TLR9 pathway which may contribute to genotoxicity in the respiratory system 65 .…”

Section: Dmentioning

confidence: 99%

New Occupational Exposures with Respiratory Effects

Moitra¹,

Haldar²

2021

BRN

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Occupational exposure is one of the major risk factors for lung diseases globally. Although the aetiology and the course of major occupational lung diseases are well understood, the nature of lung diseases caused by new agents is still not well evaluated. The rise of interstitial and malignant lung diseases due to occupational exposures has caught global attention because either the causal agents are novel and the mode of action is poorly understood, or classical exposures have reappeared in a newer way, or the control measures are not implemented properly. With the introduction of new and innovative technologies, new agents such as indium compounds, diketones, nanomaterials, and physical processes such as hydraulic fracking are being introduced with substantially high deliberating effects on the workers' health. In this review, we summarise some new occupational exposures contributing to respiratory diseases and provide some recommendations for reducing the burden of occupational lung diseases.

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Nitrative DNA damage in lung epithelial cells exposed to indium nanoparticles and indium ions

Cited by 9 publications

References 42 publications

Glycyrrhizin Attenuates Carcinogenesis by Inhibiting the Inflammatory Response in a Murine Model of Colorectal Cancer

Glycyrrhizin Attenuates Carcinogenesis by Inhibiting the Inflammatory Response in a Murine Model of Colorectal Cancer

Evaluation of the Dermal Toxicity of InZnP Quantum Dots Before and After Accelerated Weathering: Toward a Safer-By-Design Strategy

New Occupational Exposures with Respiratory Effects

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