2013
DOI: 10.1242/jeb.086694
|View full text |Cite
|
Sign up to set email alerts
|

Nitric oxide affects short-term olfactory memory in the antennal lobe ofManduca Sexta

Abstract: SUMMARYNitric oxide (NO) is thought to play an important neuromodulatory role in olfaction. We are using the hawkmoth Manduca sexta to investigate the function of NO signaling in the antennal lobe (AL; the primary olfactory network in invertebrates). We have found previously that NO is present at baseline levels, dramatically increases in response to odor stimulation, and alters the electrophysiology of AL neurons. It is unclear, however, how these effects contribute to common features of olfactory systems suc… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
1
1
1
1

Citation Types

0
17
0

Year Published

2014
2014
2022
2022

Publication Types

Select...
6
1

Relationship

3
4

Authors

Journals

citations
Cited by 12 publications
(17 citation statements)
references
References 54 publications
0
17
0
Order By: Relevance
“…By forager age, tyrosine levels were significantly lower and arginine levels were also lower (P=0.06, Tukey HSD). Tyrosine is in the biosynthesis pathways of dopamine and octopamine, and arginine is in the biosynthesis pathway of the gaseous, unconventional neurotransmitter nitric oxide, known to be involved in odor learning and memory (Müller, 1996, Gage et al, 2013. Serine, threonine and tryptophan were also reduced overall in Nosemainfected bees, though their levels were not significantly lower than those in controls at either age.…”
Section: Discussionmentioning
confidence: 99%
See 1 more Smart Citation
“…By forager age, tyrosine levels were significantly lower and arginine levels were also lower (P=0.06, Tukey HSD). Tyrosine is in the biosynthesis pathways of dopamine and octopamine, and arginine is in the biosynthesis pathway of the gaseous, unconventional neurotransmitter nitric oxide, known to be involved in odor learning and memory (Müller, 1996, Gage et al, 2013. Serine, threonine and tryptophan were also reduced overall in Nosemainfected bees, though their levels were not significantly lower than those in controls at either age.…”
Section: Discussionmentioning
confidence: 99%
“…The brain was ground manually, frozen in liquid nitrogen and transferred to −80°C until analysis. Because levels of neurotransmitters are known to fluctuate throughout the day (Kloppenburg et al, 1999;Gage et al, 2013Gage et al, , 2014, all brains (N=42) were dissected between 14:00 h and 17:00 h and each dissection was alternated between treatments to control for any time of day variances between 14:00 h and 17:00 h. The abdomen of the same bee was saved at −20°C for Nosema spore counts.…”
Section: Biogenic Amine Analysis Of Brain Tissuementioning
confidence: 99%
“…14:30 was the time point found in the Gage et al (2013) study that showed a robust effect of NO in short-term memory. 02:30 was used as a photophase counterpoint to examine memory 2.5 h after photophase/sunrise; (2) 11:30 and 23:30 were chosen because each preceded the light cycle switch (from photophase to scotophase and from scotophase to photophase) by 30 min, potentially illuminating an association between memory and the impending light cycle change; and (3) 07:30 and 19:30 were chosen as mid-phase time points, both 7.5 h into photophase and scotophase.…”
Section: Methodsmentioning
confidence: 84%
“…Given this widespread prominence, NO is thought to play a functional role in olfactory processing and behavior, yet the significance of this role is only beginning to be understood. We have previously shown that NO is necessary for short-term olfactory memory in the AL in the moth, Manduca sexta (Gage et al, 2013 ). These moths are nocturnal and heavily depend on their olfactory systems to find mates, feed, and find sites to lay eggs.…”
Section: Introductionmentioning
confidence: 96%
“…Isoleucine, methionine and phenylalanine synthesize other amino acids found to vary significantly with pollen such as glutamine, alanine, cysteine, and tyrosine (Wu, 2009). Lysine is directly involved in nitric oxide synthesis, a known neurotransmitter to affect memory in bees and moths (Muller, 1996;Gage et al, 2013;Gage and Nighorn, 2014); and leucine regulates protein turnover through cellular mTOR signaling and gene expression (Wu, 2009). Given these roles, the low variation in essential amino acids may not indicate a lack of effect.…”
Section: Essential Amino Acidsmentioning
confidence: 99%