Nitric oxide and hydrogen peroxide increase glucose-6-phosphate dehydrogenase activities and expression upon drought stress in soybean roots

Wang, Xiaomin; Ruan, Mengjiao; Wan, Qi; He, Wenliang; Yang, Lei; Liu, Xinyuan; He, Ling; Yan, Lili; Bi, Yurong

doi:10.1007/s00299-019-02473-3

Cited by 26 publications

(16 citation statements)

References 37 publications

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“…This result demonstrated that FaG6PDH positively regulated cold tolerance in strawberry ( Figure 3 ). G6PDH has been shown to respond to various oxidative stresses at the levels of enzyme activity and gene expression [ 31 , 32 ]. Meanwhile, the subcellular location of G6PDH seemed to have certain impacts on their stress responses [ 26 , 33 ].…”

Section: Discussionmentioning

confidence: 99%

Identification of the Cytosolic Glucose-6-Phosphate Dehydrogenase Gene from Strawberry Involved in Cold Stress Response

Zhang

Luo

Cheng

et al. 2020

IJMS

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Glucose-6-phosphate dehydrogenase (G6PDH) plays an important role in plant stress responses. Here, five FaG6PDH sequences were obtained in strawberry, designated as FaG6PDH-CY, FaG6PDH-P1, FaG6PDH-P1.1, FaG6PDH-P2 and FaG6PDH-P0, which were divided into cytosolic (CY) and plastidic (P) isoforms based on the bioinformatic analysis. The respective FaG6PDH genes had distinct expression patterns in all tissues and at different stages of fruit development. Notably, FaG6PDH-CY was the most highly expressed gene among five FaG6PDH members, indicating it encoded the major G6PDH isoform throughout the plant. FaG6PDH positively regulated cold tolerance in strawberry. Inhibition of its activity gave rise to greater cold-induced injury in plant. The FaG6PDH-CY transcript had a significant increase under cold stress, similar to the G6PDH enzyme activity, suggesting a principal participant in response to cold stress. Further study showed that the low-temperature responsiveness (LTR) element in FaG6PDH-CY promoter can promote the gene expression when plant encountered cold stimuli. Besides, FaG6PDH-CY was involved in regulating cold-induced activation of antioxidant enzyme genes (FaSOD, FaCAT, FaAPX and FaGR) and RBOH-dependent ROS generation. The elevated FaG6PDH-CY enhanced ROS-scavenging capability of antioxidant enzymes to suppress ROS excessive accumulation and relieved the oxidative damage, eventually improving the strawberry resistance to cold stress.

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Section: Discussionmentioning

confidence: 99%

Identification of the Cytosolic Glucose-6-Phosphate Dehydrogenase Gene from Strawberry Involved in Cold Stress Response

Zhang

Luo

Cheng

et al. 2020

IJMS

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“…In non-photosynthetic tissues, NADPH production is sourced through the OPPP [ 13 ]. Numerous literature have reported that G6PDH is widely induced in aluminum, drought, salt, and low-temperature stresses [ 23 , 24 , 25 , 26 , 27 , 28 , 29 , 30 , 33 , 34 ]. Under salinity or drought conditions, the activity of cytosolic and plastidic G6PDH significantly increases in highland barley seedlings, and the cytosolic G6PDH is more sensitive than the plastidic G6PDH ( Figure 4 ).…”

Section: Discussionmentioning

confidence: 99%

“…Numerous literature have reported that G6PDH is involved in growth and development, such as oil and lipid accumulation, seed germination, and RNA biosynthesis [ 17 , 21 , 22 ]. Moreover, G6PDH responses to abiotic stresses such as drought [ 23 , 24 ], salt [ 25 , 26 , 27 , 28 , 29 ], metal [ 30 , 31 ], heat [ 32 ], cold [ 33 , 34 ], and so on; and in the dark, plastid G6PDH could provide NADPH instead of light reactions in the photosynthetic tissues [ 35 , 36 ]. Recently, studies of G6PDH mainly focus on its activity and gene expression, as well as the regulatory mechanism under various stresses, emphasizing the role of G6PDH in maintaining cellular redox homeostasis to enhance the tolerance to environmental stresses in plants.…”

Section: Introductionmentioning

confidence: 99%

Identification, Characterization, and Stress Responsiveness of Glucose-6-phosphate Dehydrogenase Genes in Highland Barley

Feng

Wang

et al. 2020

Plants

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G6PDH provides intermediate metabolites and reducing power (nicotinamide adenine dinucleotide phosphate, NADPH) for plant metabolism, and plays a pivotal role in the cellular redox homeostasis. In this study, we cloned five G6PDH genes (HvG6PDH1 to HvG6PDH5) from highland barley and characterized their encoded proteins. Functional analysis of HvG6PDHs in E. coli showed that HvG6PDH1 to HvG6PDH5 encode the functional G6PDH proteins. Subcellular localization and phylogenetic analysis indicated that HvG6PDH2 and HvG6PDH5 are localized in the cytoplasm, while HvG6PDH1, HvG6PDH3, and HvG6PDH4 are plastidic isoforms. Analysis of enzymatic activities and gene expression showed that HvG6PDH1 to HvG6PDH4 are involved in responses to salt and drought stresses. The cytosolic HvG6PDH2 is the major isoform against oxidative stress. HvG6PDH5 may be a house-keeping gene. In addition, HvG6PDH1 to HvG6PDH4 and their encoded enzymes responded to jasmonic acid (JA) and abscisic acid (ABA) treatments, implying that JA and ABA are probably critical regulators of HvG6PDHs (except for HvG6PDH5). Reactive oxygen species analysis showed that inhibition of cytosolic and plastidic G6PDH activities leads to increased H2O2 and O2− contents in highland barley under salt and drought stresses. These results suggest that G6PDH can maintain cellular redox homeostasis and that cytosolic HvG6PDH2 is an irreplaceable isoform against oxidative stress in highland barley.

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“…At the molecular level, NO induces the expression of stress-related genes in response to drought stress. For instance, NO positively regulated the transcription of stress-adaptation-related genes, i.e., Cyt-G6PD (GPD5, G6PD6, and G6PD7) in soybean roots [75]. Zhao et al [46] found that more than 2,000 genes were differentially expressed in droughtstressed alfalfa plants treated with or without NO.…”

Section: The Involvement Of No In Drought Tolerancementioning

confidence: 99%

Plant Nitric Oxide Signaling under Drought Stress

Lau

Hamdan

Pua

et al. 2021

Plants

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Water deficit caused by drought is a significant threat to crop growth and production. Nitric oxide (NO), a water- and lipid-soluble free radical, plays an important role in cytoprotection. Apart from a few studies supporting the role of NO in drought responses, little is known about this pivotal molecular amendment in the regulation of abiotic stress signaling. In this review, we highlight the knowledge gaps in NO roles under drought stress and the technical challenges underlying NO detection and measurements, and we provide recommendations regarding potential avenues for future investigation. The modulation of NO production to alleviate abiotic stress disturbances in higher plants highlights the potential of genetic manipulation to influence NO metabolism as a tool with which plant fitness can be improved under adverse growth conditions.

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Nitric oxide and hydrogen peroxide increase glucose-6-phosphate dehydrogenase activities and expression upon drought stress in soybean roots

Cited by 26 publications

References 37 publications

Identification of the Cytosolic Glucose-6-Phosphate Dehydrogenase Gene from Strawberry Involved in Cold Stress Response

Identification of the Cytosolic Glucose-6-Phosphate Dehydrogenase Gene from Strawberry Involved in Cold Stress Response

Identification, Characterization, and Stress Responsiveness of Glucose-6-phosphate Dehydrogenase Genes in Highland Barley

Plant Nitric Oxide Signaling under Drought Stress

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