Nitric Oxide and Modulatory Effects of the Root extracts of Phlogacanthus tubiflorus against Oxidative Stress induced by Hydrogen Peroxide

Hussain, Abul; Saikia, Viveeyan; Ramteke, Anand

doi:10.5530/ax.2012.2.4

Cited by 10 publications

(4 citation statements)

References 16 publications

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“…High levels of NO cause oxidative damage to cells and induce apoptosis [42]. Hussain et al have demonstrated that the NO level was significantly increased in H 2 O 2 -treated lymphocytes [43]. In the present study, the increased NO production induced by H 2 O 2 was suppressed by the treatment of TC and the combination but not the treatment of CTS, suggesting that the CTS and TC combination might show a protective effect on the production of NO.…”

Section: Discussionsupporting

confidence: 48%

Carthamus tinctorius L. Seed and Taraxacum coreanum Attenuate Oxidative Stress Induced by Hydrogen Peroxide in SH-SY5Y Cells

He,

Park,

Shin

et al. 2023

Foods

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Oxidative stress is closely associated with the pathology of neurodegenerative diseases. The seeds of Carthamus tinctorius L. (CTS) and Taraxacum coreanum (TC) are reported as herbal medicines for neuroprotection. This study investigated the protective effect of CTS, TC, and their combination against oxidative stress induced by H2O2 in SH-SY5Y cells. The CTS and TC combination dose-dependently increased DPPH and ·OH radical scavenging activities compared with non-combination. The combination showed a higher increased cell survival rate in H2O2-stimulated SH-SY5Y cells than CTS or TC. Moreover, CTS, TC, and their combination-treated cells reduced LDH release and apoptotic cells. CTS, TC, and their combination also inhibited NO and ROS generation. Further, the combination of up-regulated antioxidant enzymes (superoxide dismutase and glutathione peroxidase) and Bcl-2 protein expressions and down-regulated Bax expression. These findings suggest that the combination of CTS and TC may be beneficial to prevent and treat oxidative stress-mediated neurodegenerative diseases.

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Section: Discussionsupporting

confidence: 48%

Carthamus tinctorius L. Seed and Taraxacum coreanum Attenuate Oxidative Stress Induced by Hydrogen Peroxide in SH-SY5Y Cells

He,

Park,

Shin

et al. 2023

Foods

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“…It was diluted in the ratio of 1 : 1 with phosphate buffer saline (PBS) and layered 6 mL into 6 mL histopaque (1.077 g/mL). The isolation of lymphocytes and study of cell viability were carried out as per the procedure stated in the literature . Lymphocytes were isolated from the sample after centrifugation for 30 min at 400 g, washed with PBS, and finally with serum‐free media separately through centrifugation for 10 min at 250 g. Cell pellets were then suspended in PBS, and cell viability was checked by Trypan blue exclusion method using haemocytometer.…”

Section: Methodsmentioning

confidence: 99%

Carboxymethyl chitosan-montmorillonite nanoparticles for controlled delivery of isoniazid: evaluation of the effect of the glutaraldehyde and montmorillonite

Banik

Ramteke

Maji

2014

Polym. Adv. Technol.

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Chitosan, a natural biopolymer, is used for drug delivery application. But its potential application is limited by its low solubility in aqueous media. The present study was designed to prepare carboxymethyl chitosan (CMC), a water soluble derivative of chitosan, and evaluate the prospective of crosslinked CMC-Montmorillonite (MMT) nanoparticles for controlled delivery of isoniazid. The nanoparticles were characterized by Fourier Transmission Infrared Spectroscopy (FTIR), Nuclear Magnetic Resonance (NMR), X-ray diffraction (XRD), scanning electron microscopy (SEM), and Transmission emission microscopy (TEM). The effects of MMT and glutaraldehyde on nanoparticles were assessed with regard to encapsulation efficiency, percentage swelling degree, and cumulative release. Percentage swelling degree and cumulative release were studied in pH medium 1.2 and 7.4 for 6 h. The cumulative release was studied by UV-visible spectrophotometer. Cell viability study was performed by MTT assay analysis. FTIR and NMR study indicated the successful preparation of CMC. FTIR study confirmed the interaction of MMT with CMC. The exfoliation of MMT layers and molecular level dispersion of isoniazid in CMC was examined by XRD and TEM. SEM study showed that the surface of the CMC-MMT nanoparticles was smooth compared with those of CMC nanoparticles. Swelling and release of isoniazid from the nanoparticles increased with the decrease in the MMT and glutaraldehyde content. The percentage swelling degree and cumulative release was more in pH 1.2. Cell viability study revealed that CMC was not cytotoxic, and the nanoparticles containing MMT was less cytotoxic than those of MMT free nanoparticles. CMC-MMT nanoparticles can be exploited as potential drug carrier for controlled release applications.

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“…The isolation of lymphocytes and study of cell viability were done as per the procedure stated in the literature. 28 Lymphocytes were…”

Section: Isolation Of Lymphocytes Culture and Treatmentmentioning

confidence: 99%

Soy flour nanoparticles for controlled drug delivery: effect of crosslinker and montmorillonite (MMT)

et al. 2013

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Soy flour (SF)-Montmorillonite (MMT) nanoparticles crosslinked with glutaraldehyde (GA) have been made and used as a carrier for isoniazid. The nanoparticles have been characterized by fourier transmission infra-red spectroscopy (FTIR), X-ray diffractometry (XRD), scanning electron microscopy (SEM) and transmission emission microscopy (TEM). The effects of MMT and glutaraldehyde on the nanoparticles have been assessed with regard to swelling, encapsulation efficiency and consequently, the release of isoniazid in different mediums.The drug release mechanism has been studied for different time periods by UV-Vis spectroscopy. Cytotoxicity testing has been performed by MTT assay analysis. The results imply that the nanoparticles can be exploited as a potential drug carrier for controlled release applications.

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Nitric Oxide and Modulatory Effects of the Root extracts of Phlogacanthus tubiflorus against Oxidative Stress induced by Hydrogen Peroxide

Cited by 10 publications

References 16 publications

Carthamus tinctorius L. Seed and Taraxacum coreanum Attenuate Oxidative Stress Induced by Hydrogen Peroxide in SH-SY5Y Cells

Carthamus tinctorius L. Seed and Taraxacum coreanum Attenuate Oxidative Stress Induced by Hydrogen Peroxide in SH-SY5Y Cells

Carboxymethyl chitosan-montmorillonite nanoparticles for controlled delivery of isoniazid: evaluation of the effect of the glutaraldehyde and montmorillonite

Soy flour nanoparticles for controlled drug delivery: effect of crosslinker and montmorillonite (MMT)

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