Nitric Oxide/cGMP Mediates the Spasmolytic Action of 3,4′-Dihydroxy-5,5′-dimethoxybibenzyl fromScaphyglottis livida

Estrada, Samuel; Rojas, Antonio; Mathison, Yaira; Israel, A; Mata, Rachel

doi:10.1055/s-1999-14056

Cited by 49 publications

(61 citation statements)

References 5 publications

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“…In the present study, the sustained contraction of the muscle caused by high concentration of The second messenger, nitric oxide, is an important candidate for mediating non-adrenergic and non-cholinergic smooth muscle relaxation in the gastrointestinal tract (Estrada et al, 1999;Takahashi, 2003;Estrada-Soto et al, 2010). Therefore, we explored the possibility that NO was involved in the antispasmodic activity displayed by citral and LE on the spontaneous contractions.…”

Section: Discussionmentioning

confidence: 95%

Spasmolytic effect of citral and extracts of Cymbopogon citratus on isolated rabbit ileum

Devi

Sim

Ismail

2011

J Smooth Muscle Res

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Cymbopogon citratus, commonly known as lemongrass, has been shown to have antioxidant, antimicrobial and chemo-protective properties. Citral, a monoterpenoid, is the major constituent of C. citratus that gives off a lemony scent and is postulated to be responsible for most of its actions. In addition, C. citratus has been traditionally used to treat gastrointestinal discomforts, however, the scientific evidence for this is still lacking. Thus, the aim of the present study was to investigate the effect of the extracts of various parts of C. citratus (leaves, stems and roots) and citral on the visceral smooth muscle activity of rabbit ileum. The effect of the test substances were tested on the spontaneous contraction, acetylcholine (ACh)-and KCl-induced contractions. Citral at doses between 0.061 mM to 15.6 mM and the extract of leaves at doses between 0.001 mg/mL to 1 mg/mL significantly reduced the spontaneous, ACh-and KCl-induced ileal contractions. When the ileum was incubated in K + -rich-Ca 2+ -free Tyrode's solution, it showed only minute contractions. However, the strength of contraction was increased with the addition of increasing concentrations of CaCl2. The presence of citral almost abolished the effect of adding CaCl2, while the leaf extract shifted the calcium concentration-response curve to the right, suggesting a calcium antagonistic effect. These results were similar to that elicited by verapamil, a known calcium channel blocker. In addition, the spasmolytic effect of citral was observed to be reduced by the nitric oxide synthase inhibitor, L-NAME. In conclusion, citral and the leaf extract of C. citratus exhibited spasmolytic activity and it appeared that they may act as calcium antagonists. Furthermore, the relaxant effect of citral, but not that of the leaf extract may be mediated by nitric oxide suggesting the presence of other chemical components in the leaf extract other than citral.

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Section: Discussionmentioning

confidence: 95%

Spasmolytic effect of citral and extracts of Cymbopogon citratus on isolated rabbit ileum

Devi

Sim

Ismail

2011

J Smooth Muscle Res

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“…This dihydrostilbenoid increases cyclic GMP content in the rat ileum muscle ring. Formation of nitric oxide/cGMP constitutes the signaling pathway in the spasmolytic action of gigantol [11,12].…”

Section: Discussionmentioning

confidence: 99%

Relaxant and antispasmodic effects of extracts of the orchid Encyclia michuacana on isolated guinea pig ileum

Gutierrez

Solís

2008

J Nat Med

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The antispasmodic effects of hexane-, chloroform-, methanol- and water-extracts of the orchid Encyclia michuacana were studied in vitro on guinea pig ileum against three spasmogens: acetylcholine (Ach), histamine and barium chloride. The chloroform extract exerted a significant antispasmodic effect on ileum contractions induced by Ach, histamine and barium chloride (IC(50) = 90.64, 73.12 and 115.2 microg/mL, respectively). Furthermore, the chloroform extract of E. michuacana provoked a concentration-dependent inhibition of spontaneous contractions of guinea pig ileum with potencies comparable to those of papaverine. The antagonism against the spasmogens used suggests that the action of the chloroform extract of E. michuacana could be due mainly to the presence of gigantol. Hexane-, methanol- and water-extracts did not elicit any significant spasmolytic activity.

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“…Anti-inflammatory, anti-nociceptive [28,29] and relaxing activities [30,31] detected in Scaphyglottis livida, a close related species. Lanostadienol derivative, gigantol, ciclobalanone [29].…”

Section: Scaphyglottis Fasciculata Hookmentioning

confidence: 99%

“…Lanostadienol derivative, gigantol, ciclobalanone [29]. Phenanthrenederivati ve [28,30] and 3, 4-dihydroxi-5,5'-dimet hoxy bibenzyl [30].…”

Section: Scaphyglottis Fasciculata Hookmentioning

confidence: 99%

“…Nevertheless, the proven pharmacological effects in a related species, S. livida, include anti-inflammatory [28], anti-nociceptive [29] and smooth muscle relaxing activity [30,31]. The responsible compounds were a lanostadienol derivative, gigantol, ciclobalanone [29], phenanthrene derivatives [28,30] and 3,4-dihydroxi-5,5'-dimetoxy benzyl [30]. The above activities, confirmed through pharmacological testing, might have an overall positive effect that could be related to the therapeutic application of S.…”

Section: As Far As Their Ethnobotany Villagers Usementioning

confidence: 99%

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Ethnobotany, Pharmacology and Chemistry of Medicinal Orchids from Veracruz

Asseleih¹,

García²,

Cruz³

2015

JAST-A

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Nitric Oxide/cGMP Mediates the Spasmolytic Action of 3,4^′-Dihydroxy-5,5^′-dimethoxybibenzyl fromScaphyglottis livida

Cited by 49 publications

References 5 publications

Spasmolytic effect of citral and extracts of Cymbopogon citratus on isolated rabbit ileum

Spasmolytic effect of citral and extracts of Cymbopogon citratus on isolated rabbit ileum

Relaxant and antispasmodic effects of extracts of the orchid Encyclia michuacana on isolated guinea pig ileum

Ethnobotany, Pharmacology and Chemistry of Medicinal Orchids from Veracruz

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