Nitric oxide-dependent vasorelaxation induced by extractive solutions and fractions of Maytenus ilicifolia Mart ex Reissek (Celastraceae) leaves

Rattmann, Yanna Dantas; Cipriani, Thales R.; Sassaki, Guilherme L.; Iacomini, Marcello; Rieck, Lia; Marques, Maria Consuelo Andrade; Silva-Santos, José Eduardo da

doi:10.1016/j.jep.2005.09.026

Cited by 29 publications

(18 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Under physiological conditions, NO is continuously released by vascular endothelial cells as a consequence of the shear stress generated by blood flow [15] . Nonetheless, the release of NO can also be stimulated by muscarinic receptor activation [16] .…”

Section: Discussionmentioning

confidence: 99%

In vitro Vascular Effects Produced by Crude Aqueous Extract of Green Marine Algae, Cladophora patentiramea (Mont.) Kützing, in Aorta from Normotensive Rats

Lim¹,

Mok²

2010

Med Princ Pract

View full text Add to dashboard Cite

Objectives: To investigate the antihypertensive activity of aqueous extracts obtained from Malaysian coastal seaweeds and to determine the pharmacological mechanisms of the extracts on rat aorta in vitro. Materials and Methods: The antihypertensive activity of 11 species of seaweeds (5 brown, 1 red and 5 green algae) were tested by cumulative addition of the extracts to phenylephrine (PE)-precontracted Wistar-Kyoto (WKY) aortic rings in in vitro isometric contraction studies. Mechanisms for vasorelaxant effect were investigated in the presence of various antagonists. Results: Of the 11 species tested, 2 showed a vasorelaxant effect. Further investigation of the mechanisms of action of the aqueous extract of green alga, Cladophora patentiramea (AECP),showed that the vascular relaxant effect was endothelium- and concentration-dependent. A maximum relaxation of 45.8 ± 4.6% (n = 8, p < 0.001) was obtained at 0.1 mg/ml of extract, after which the response was found to reduce in a concentration-dependent manner to 15.7 ± 4.9% (n = 8, p < 0.001) at the highest extract concentration tested. Pretreatment of endothelium-intact aortic rings with Nω-nitro-L-arginine methyl ester (L-NAME, 30 µM), ¹H-[1,2,4]oxadiazolo[4,3-a]quinoxalin-1-one (ODQ, 10 µM) and methylene blue (100 µM) resulted in a complete blockade of AECP-induced vasorelaxation. However, the relaxant effects of the extract were not blocked by atropine (1 µM), indomethacin (10 µM) and glibenclamide (10 µM), although the maximum relaxant responses were enhanced in the presence of glibenclamide. Conclusion: Our data showed that the in vitro vascular relaxant effect of AECPwas mediated through endothelium-dependent nitric oxide-cGMP pathway, and was not associated with the release of vasodilator prostaglandins, activation of muscarinic receptors, or ATP-sensitive potassium channels opening.

show abstract

Section: Discussionmentioning

confidence: 99%

In vitro Vascular Effects Produced by Crude Aqueous Extract of Green Marine Algae, Cladophora patentiramea (Mont.) Kützing, in Aorta from Normotensive Rats

Lim¹,

Mok²

2010

Med Princ Pract

View full text Add to dashboard Cite

show abstract

“…A hydroalcoholic extract of pulverized air-dried leaves (0.1 g/ mL, 200 μm particle size) of M. ilicifolia and samples (12) were prepared in water:ethanol (30:70, v/v), at room temperature for 7 days, in darkness. The samples were previously filtered through a 0.5 μm pore size filter (Millipore, Bedford, USA) prior to injection.…”

Section: Preparation Of Standard and Sample Extractsmentioning

confidence: 99%

“…Normally these actions are attributed to the presence of phenolic metabolites and triterpenes. [9][10][11][12][13][14][15][16] The ANVISA (National Agency of Sanitary Surveillance -Brazil) has approved, for the treatment of gastric disorders, the use and sale of phytomedicines from M. ilicifolia, which are standardized only by their tannin. 7,17 For this reason, many Brazilian pharmaceutical industries and shops, produce and market phytopharmaceuticals containing this plant.…”

Section: Introductionmentioning

confidence: 99%

A quantitative validated method using liquid chromatography and chemometric analysis for evaluation of raw material oF Maytenus ilicifolia (Schrad.) Planch., Celastraceae

Beltrame¹,

Mainardes²,

Khalil³

et al. 2012

Quím. Nova

View full text Add to dashboard Cite

Recebido em 28/3/11; aceito em 2/7/11; publicado na web em 19/8/11The hydroalcoholic extracts prepared from standard leaves of Maytenus ilicifolia and commercial samples of espinheira-santa were evaluated qualitatively (fingerprinting) and quantitatively. In this paper, fingerprinting chromatogram coupled with Principal Component Analysis (PCA) is described for the metabolomic analysis of standard and commercial espinheira-santa samples. The epicatechin standard was used as an external standard for the development and validation of a quantitative method for the analysis in herbal medicines using a photo diode array detector. This method has been applied for quantification of epicatechin in commercialized herbal medicines sold as espinheira-santa in Brazil and in the standard sample of M. ilicifolia.

show abstract

“…Maytenus ilicifolia (Celastraceae) popularly known in Brazil as "espinheira-santa" (holy spines) is a native plant from southern Brazil, Paraguay, Uruguay and northern Argentina [1]. It is used in folk medicine for the treatment of dyspepsia as well as gastric ulcers and potentially on cancer treatment [2]- [4] Cipriani et al demonstrated, after several purification steps, that the tea (infusion) of M. ilicifolia leaves releases a polysaccharide (arabinogalactan).…”

Section: Introductionmentioning

confidence: 99%

Anti-ulcerogenic Action Mechanism of Maytenus ilicifolia Detected by mRNA RT-PCR and Free Radical Scavenging Activity Evaluated by ESR

Pazzini¹

2017

AJHR

View full text Add to dashboard Cite

Abstract:Maytenus ilicifolia, known as "espinheira-santa", is used in the popular medicine for peptic ulcer treatment.This work evaluates the preventive and curative actions of M. ilicifolia in animals, the healing activity by EGF expression detected by RT-PCR as well as the anti-oxidative activity by Electron Spin Resonance spectroscopy (ESR). Oral administration of M. ilicifolia (300 and 500 mg/kg, p.o.) one hour before the ulcerogenic agent application prevented the ulcer formation 42 ± 0.98% and 72 ± 1.2% (p <0.05 and p <0.001, respectively), in comparison with the negative control tween 80, preserving the cytoprotection characteristics of the gastric mucosa and assuring the integrity of gastric glands and gastric fossets. The animals treated with tween, had the epithelium and the mucosa layer damaged and accentuated vascularization. The healing activity of M. ilicifolia (500mg/kg, p.o.) was 71 ± 1.4% (p <0.001) in chronic ulcer experiments induced by acetic acid. EGF expression detected by RT-PCR confirmed the healing activity. Histological analysis showed the recovery of the mucosal layer and the epithelium surface harmed by the acetic acid. Studies in vitro by Electron Spin Resonance spectroscopy assessed the anti-oxidative action of M ilicifolia. Experiments with DPPH (2.2-Diphenyl -1-picrylhydrazyl) demonstrated that the polar fraction of M. ilicifolia presents an IC 50 of 0.68±0.09 mg/ml. The value of 9±2 mg/ml was found for IC 50 in experiments with the radical OH, produced by the Fenton Reaction and detected through spin adduct DMPO-OH. Mass spectrometry analysis of crude extracts and fractions from M. ilicifolia was carried out and compounds of pentacyclic triterpenes class were identified.

show abstract

Nitric oxide-dependent vasorelaxation induced by extractive solutions and fractions of Maytenus ilicifolia Mart ex Reissek (Celastraceae) leaves

Cited by 29 publications

References 31 publications

In vitro Vascular Effects Produced by Crude Aqueous Extract of Green Marine Algae, <i>Cladophora patentiramea</i> (Mont.) Kützing, in Aorta from Normotensive Rats

In vitro Vascular Effects Produced by Crude Aqueous Extract of Green Marine Algae, <i>Cladophora patentiramea</i> (Mont.) Kützing, in Aorta from Normotensive Rats

A quantitative validated method using liquid chromatography and chemometric analysis for evaluation of raw material oF Maytenus ilicifolia (Schrad.) Planch., Celastraceae

Anti-ulcerogenic Action Mechanism of <i>Maytenus ilicifolia</i> Detected by mRNA RT-PCR and Free Radical Scavenging Activity Evaluated by ESR

Contact Info

Product

Resources

About

Nitric oxide-dependent vasorelaxation induced by extractive solutions and fractions of Maytenus ilicifolia Mart ex Reissek (Celastraceae) leaves

Cited by 29 publications

References 31 publications

In vitro Vascular Effects Produced by Crude Aqueous Extract of Green Marine Algae, <i>Cladophora patentiramea</i> (Mont.) Kützing, in Aorta from Normotensive Rats

In vitro Vascular Effects Produced by Crude Aqueous Extract of Green Marine Algae, <i>Cladophora patentiramea</i> (Mont.) Kützing, in Aorta from Normotensive Rats

A quantitative validated method using liquid chromatography and chemometric analysis for evaluation of raw material oF Maytenus ilicifolia (Schrad.) Planch., Celastraceae

Anti-ulcerogenic Action Mechanism of &lt;i&gt;Maytenus ilicifolia&lt;/i&gt; Detected by mRNA RT-PCR and Free Radical Scavenging Activity Evaluated by ESR

Contact Info

Product

Resources

About

Anti-ulcerogenic Action Mechanism of <i>Maytenus ilicifolia</i> Detected by mRNA RT-PCR and Free Radical Scavenging Activity Evaluated by ESR