Nitric oxide-induced mutations in theHPRT gene of human lymphoblastoid TK6 cells and inSalmonella typhimurium

Zhuang, John C.; Wright, Teresa L.; deRojas-Walker, Teresa; Tannenbaum, Steven R.; Wogan, Gerald N.

doi:10.1002/(sici)1098-2280(2000)35:1<39::aid-em6>3.0.co;2-h

Cited by 40 publications

(22 citation statements)

References 43 publications

(59 reference statements)

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“…1B), consistent with earlier findings (16,17,19,21). Continuous exposure of TK6 cells for 2 h, at a rate of 533 nM/s NO • , caused a 2-fold increase in HPRT and 4.2-fold in TK1 gene expression as compared with argon-treated controls (17,21).…”

Section: Discussionsupporting

confidence: 90%

“…A:T to G:C transitions, generally induced by oxidative damage (19), were also frequently observed (Table II). Deamination of adenine to hypoxanthine, which pairs with cytosine rather than thymine in DNA, could account for the high proportion of A:T to G:C transitions observed (19). The predominance of G:C to T:A transversions is consistent with previously reported mutation types induced by peroxynitrite (ONOO -) and its derivatives generated by reaction of NO • with O 2…”

Section: Proportion Of Mutants (mentioning

confidence: 92%

“…• gas induced G:C to T:A, T:A to A:T, and C:G to A:T mutations (19), and also mutagenized TK6 cells, causing A:T to T:A and A:T to G:C mutations in the HPRT gene (19). Peroxynitrite, the reaction product of NO…”

Section: Proportion Of Mutants (mentioning

confidence: 99%

“…ROS-associated G:C to T:A transversions may arise from oxidative damage, leading to dA rather than dC being incorporated opposite dG. A:T to G:C transitions, generally induced by oxidative damage (19), were also frequently observed (Table II). Deamination of adenine to hypoxanthine, which pairs with cytosine rather than thymine in DNA, could account for the high proportion of A:T to G:C transitions observed (19).…”

Section: Proportion Of Mutants (mentioning

confidence: 99%

“…• and ROS-associated genotoxicity have been performed by our group (14)(15)(16)(17)(18)(19)(20). The effects of DNA damage, mutational frequencies and spectra induced by varying bolus doses of ONOO -were compared with those induced by slow infusion of ONOO -and by SIN-1, which slowly decomposes to release NO…”

Section: Introductionmentioning

confidence: 99%

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Intracellular and extracellular factors influencing the genotoxicity of nitric oxide and reactive oxygen species

Kim

2017

Oncology Letters

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Abstract. A number of factors affect cellular responses to nitric oxide (NO • ) and reactive oxygen species (ROS), including their source, concentration, cumulative dose, target gene and biological milieu. This limits the extrapolation of data to in vivo pathological states in which NO• and ROS may be important. The present study investigated lethality and mutagenesis in the HPRT and TK1 genes of human lymphoblastoid TK6 cells exposed to NO• and ROS derived from two delivery methods: A reactor system and a Transwell™ co-culture. The delivery of NO• into the medium at controlled steady-state concentrations (given in µM/min) and the production of NO• and ROS by activated macrophages, resulted in a time-dependent decrease in total cell numbers, and an increase in mutation frequency (MF), compared with untreated controls. This increase in MF was effectively suppressed by N-methyl-L-arginine monoacetate. Single base substitutions were the most common type of spontaneous and NO• induced mutations in HPRT, followed by exon exclusions and small deletions in both delivery systems. Among the single base pair substitutions, an equal frequency of four types of single base substitutions were identified in TK6 cells exposed to NO• delivered by the reactor system, whereas G:C to T:A transversions and A:T to G:C transitions were more frequent in the co-culture system. Taken together, these results demonstrate that both the delivery method of NO • and ROS, and the target genes are determinants of observed cytotoxic and mutagenic responses, indicating that these parameters need to be considered in assessing the potential effects of NO• and ROS in vivo.

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Section: Discussionsupporting

confidence: 90%

Section: Proportion Of Mutants (mentioning

confidence: 92%

Section: Proportion Of Mutants (mentioning

confidence: 99%

Section: Proportion Of Mutants (mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Intracellular and extracellular factors influencing the genotoxicity of nitric oxide and reactive oxygen species

Kim

2017

Oncology Letters

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Stickstoffmonoxid [MAK Value Documentation in German language, 2010]

2012

The MAK‐Collection for Occupational Health and Safety

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Veröffentlicht in der Reihe Gesundheitsschädliche Arbeitsstoffe , 49. Lieferung, Ausgabe 2010 Der Artikel enthält folgende Kapitel: Allgemeiner Wirkungscharakter Wirkungsmechanismus Toxikokinetik und Metabolismus Endogene Bildung von NO Aufnahme, Verteilung, Ausscheidung Metabolismus Erfahrungen beim Menschen Einmalige Exposition Wiederholte Exposition Wirkung auf Haut und Schleimhäute Allergene Wirkung Reproduktionstoxizität Genotoxizität Kanzerogenität Tierexperimentelle Befunde und In‐vitro‐Untersuchungen Akute Toxizität Subakute, subchronische und chronische Toxizität Wirkung auf Haut und Schleimhäute Allergene Wirkung Reproduktionstoxizität Genotoxizität Kanzerogenität Bewertung

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Nitrogen monoxide [MAK Value Documentation, 2010]

2014

The MAK‐Collection for Occupational Health and Safety

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Published in the series Occupational Toxicants , 2014 The article contains sections titled: Toxic Effects and Mode of Action Mechanism of Action Reactions of nitrogen monoxide (NO) in the cell Modes of action of nitrogen monoxide (NO) in the organism Mode of action of nitrogen monoxide (NO) compared with that of nitrogen dioxide (NO 2 ) Toxicokinetics and Metabolism Endogenous formation of nitrogen monoxide (NO) Absorption, distribution, elimination Metabolism Effects in Humans Single exposures Repeated exposure Local effects on skin and mucous membranes Allergenic effects Reproductive toxicity Genotoxicity Carcinogenicity Animal Experiments and in vitro Studies Acute toxicity Subacute, subchronic and chronic toxicity Local effects on skin and mucous membranes Allergenic effects Reproductive toxicity Genotoxicity Carcinogenicity Manifesto (MAK value, classification)

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Nitric oxide-induced mutations in theHPRT gene of human lymphoblastoid TK6 cells and inSalmonella typhimurium

Cited by 40 publications

References 43 publications

Intracellular and extracellular factors influencing the genotoxicity of nitric oxide and reactive oxygen species

Intracellular and extracellular factors influencing the genotoxicity of nitric oxide and reactive oxygen species

Stickstoffmonoxid [MAK Value Documentation in German language, 2010]

Nitrogen monoxide [MAK Value Documentation, 2010]

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