Nitric oxide inhibits larval settlement in <i>Amphibalanus amphitrite</i> cyprids by repressing muscle locomotion and molting

Zhang, Gen; Wong, Yue-Him; He, Lisheng; Xü, Ying; Qian, Pei‐Yuan

doi:10.1002/pmic.201500112

Cited by 14 publications

(15 citation statements)

References 49 publications

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“…It has been hypothesized that SIPC binds tightly to shell components (such as chitin or minerals), because a strong buffer (containing SDS and DTT) is required to extract SIPC from barnacle shells 16 . Moreover, inhibition of p38 MAPK pathway 17 and elevation of endogenous nitric oxide concentration 18 both decrease the expression level of SIPC in cyprids. It is difficult to attribute these results to the two known functions of SIPC (pheromone and adhesive).…”

mentioning

confidence: 97%

Secretory locations of SIPC in Amphibalanus amphitrite cyprids and a novel function of SIPC in biomineralization

Zhang

Yang

Leung

et al. 2016

Sci Rep

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Settlement-inducing protein complex (SIPC) is a pheromone that triggers conspecific larval settlement in the barnacle Amphibalanus amphitrite. In the present study, immunostaining and scanning electron microscopy of SIPC revealed signals in the frontal horn pores and the secretions from carapace pores, suggesting that SIPC might be directly secreted from these organs in A. amphitrite cyprids. Further observations showed that the frontal horn pores could contact surfaces while cyprids were “walking”. Immunostaining for SIPC on the contacted surfaces displayed SIPC signals. These signals were similar to the frontal horn pores in size and morphology, suggesting that frontal horn pores might deposit SIPC. Besides, full-length SIPC was expressed and subsequent assays indicated that recombinant SIPC was able to bind to chitins and induce the precipitation of CaCO3. Furthermore, recombinant SIPC inhibited the formation of vaterites and regulated the morphology of calcite crystals. The crystals that formed with recombinant SIPC were more stable against water erosion. Overall, these results reported a novel function of recombinant SIPC that regulates crystal formation in barnacle shells.

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confidence: 97%

Secretory locations of SIPC in Amphibalanus amphitrite cyprids and a novel function of SIPC in biomineralization

Zhang

Yang

Leung

et al. 2016

Sci Rep

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“…Synthesised NO at the base of the foot by NOS, which is transported or diffuses into the foot, activates the sGC to produce cGMP. Nitric oxide activating sGC and raising intracellular cGMP concentration has also been suggested for other invertebrate larvae (13,(22)(23)(24)73). The presence of cGMP in all larval stages that have a developed foot further suggests that cGMP ful ls a more generic function than exclusively being involved in regulating metamorphosis induction.…”

Section: Discussionmentioning

confidence: 89%

Bivalves are NO Different: Nitric Oxide as Negative Regulator of Metamorphosis in the Pacific Oyster, Crassostrea Gigas.

Vogeler

Carboni

et al. 2020

Preprint

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Background Nitric oxide (NO) is a presumed regulator of metamorphosis in many invertebrate species, and although NO pathways have been comparatively well-investigated in gastropods, annelids and crustaceans, there has been very limited research on the effects of NO on metamorphosis in bivalve shellfish.Results In this paper, we investigate the effects of NO pathway inhibitors and NO donors on metamorphosis induction in larvae of the Pacific oyster, Crassostrea gigas, demonstrating that the nitric oxides synthase (NOS) inhibitors s-methylisothiourea hemisulfate salt (SMIS), aminoguanidine hemisulfate salt (AGH) and 7-nitroindazole (7-NI) induce metamorphosis in a concentration-dependent manner ranging from 75%, 76–83% respectively. Additional induction resulted from exposures to 1H-[1, 2, 4]Oxadiazole[4,3-a]quinoxalin-1-one (ODQ), an inhibitor of soluble guanylyl cyclase, with which NO interacts to catalyse the synthesis of cyclic guanosine monophosphate (cGMP). Conversely, high concentrations of the NO donor sodium nitroprusside dihydrate in combination with metamorphosis inducers epinephrine, MK-801 or SMIS, significantly decreased metamorphosis. Expression of CgNOS also decreased in larvae after metamorphosis regardless of the inducers used, but intensified again post-metamorphosis in spat. Fluorescent detection of NO in competent larvae with DAF-FM diacetate and localisation of the oyster nitric oxide synthase CgNOS expression by in-situ hybridisation showed that NO occurs primarily in two key larval structures, the velum and foot. cGMP was also detected in the foot using immunofluorescent assays, and is potentially involved in the foot’s smooth muscle relaxation.Conclusion Together, these results suggest that NO is a negative regulator of metamorphosis in Pacific oyster larvae, and that NO reduction induces metamorphosis by inhibiting swimming or crawling behaviour, in conjunction with a cascade of additional neuroendocrine downstream responses.

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“…Synthesised NO at the base of the foot by NOS, which is transported or diffuses into the foot, activates the sGC to produce cGMP. Nitric oxide activating sGC and raising intracellular cGMP concentration has also been suggested for other invertebrate larvae (14,(23)(24)(25)74). The presence of cGMP in all larval stages that have a developed foot further suggests that cGMP ful ls a more generic function than exclusively being involved in regulating metamorphosis induction.…”

Section: Discussionmentioning

confidence: 89%

Bivalves are NO different: nitric oxide as negative regulator of metamorphosis in the Pacific oyster, Crassostrea gigas.

Vogeler

Carboni

et al. 2020

Preprint

View full text Add to dashboard Cite

Background: Nitric oxide (NO) is presumed to be a regulator of metamorphosis in many invertebrate species, and although NO pathways have been comparatively well-investigated in gastropods, annelids and crustaceans, there has been very limited research on the effects of NO on metamorphosis in bivalve shellfish.Results: In this paper, we investigate the effects of NO pathway inhibitors and NO donors on metamorphosis induction in larvae of the Pacific oyster, Crassostrea gigas. The nitric oxides synthase (NOS) inhibitors s-methylisothiourea hemisulfate salt (SMIS), aminoguanidine hemisulfate salt (AGH) and 7-nitroindazole (7-NI) induced metamorphosis ranging from 75%, 76% to 83% respectively, and operating in a concentration-dependent manner. Additional induction of up to 54% resulted from exposures to 1H-[1,2,4]Oxadiazole[4,3-a]quinoxalin-1-one (ODQ), an inhibitor of soluble guanylyl cyclase, with which NO interacts to catalyse the synthesis of cyclic guanosine monophosphate (cGMP). Conversely, high concentrations of the NO donor sodium nitroprusside dihydrate in combination with metamorphosis inducers epinephrine, MK-801 or SMIS, significantly decreased metamorphosis, although a potential harmful effect of excessive NO unrelated to metamorphosis pathway cannot be excluded. Expression of CgNOS also decreased in larvae after metamorphosis regardless of the inducers used, but intensified again post-metamorphosis in spat. Fluorescent detection of NO in competent larvae with DAF-FM diacetate and localisation of the oyster nitric oxide synthase CgNOS expression by in-situ hybridisation showed that NO occurs primarily in two key larval structures, the velum and foot. cGMP was also detected in the foot using immunofluorescent assays, and is potentially involved in the foot’s smooth muscle relaxation.Conclusion: Together, these results suggest that the NO pathway acts as negative regulator of metamorphosis in Pacific oyster larvae, and that NO reduction induces metamorphosis by inhibiting swimming or crawling behaviour, in conjunction with a cascade of additional neuroendocrine downstream responses.

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Nitric oxide inhibits larval settlement in Amphibalanus amphitrite cyprids by repressing muscle locomotion and molting

Cited by 14 publications

References 49 publications

Secretory locations of SIPC in Amphibalanus amphitrite cyprids and a novel function of SIPC in biomineralization

Secretory locations of SIPC in Amphibalanus amphitrite cyprids and a novel function of SIPC in biomineralization

Bivalves are NO Different: Nitric Oxide as Negative Regulator of Metamorphosis in the Pacific Oyster, Crassostrea Gigas.

Bivalves are NO different: nitric oxide as negative regulator of metamorphosis in the Pacific oyster, Crassostrea gigas.

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