Nitric oxide is involved in nano-titanium dioxide-induced activation of antioxidant defense system and accumulation of osmolytes under water-deficit stress in Vicia faba L.

Khan, Md. Raisuddin; AlSolami, Mazen A.; Basahi, Riyadh A.; Siddiqui, Manzer H.; Al-Huqail, Asma A.; Abbas, Zahid Khorshid; Siddiqui, Zahid Hameed; Ali, Hayssam M.; Khan, Faheema

doi:10.1016/j.ecoenv.2019.110152

Cited by 86 publications

(31 citation statements)

References 96 publications

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“…NO is a signalling molecule that plays a crucial role in enhancing plant tolerance in harsh environments (Fancy et al 2017, Sharma et al 2020), e.g. arsenic toxicity (Singh et al 2017a), salt stress (Arora and Bhatla 2017), heat stress (Alamri et al 2019) and sulphur deficiency (Siddiqui et al 2020) as well as WS (Cechin et al 2015, Hasanuzzaman et al 2017a, Munawar et al 2019, Khan et al 2020). However, so far no investigation have focused on the function of NR‐triggered NO in SA‐induced WS tolerance in pepper plants by upraising the a scorbate‐glutathione (AsA‐GSH) cycle and glyoxalase system.…”

Section: Introductionmentioning

confidence: 99%

Nitrate reductase is required for salicylic acid‐induced water stress tolerance of pepper by upraising the AsA‐GSH pathway and glyoxalase system

Kaya

2020

Physiologia Plantarum

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A trial was conducted to evaluate whether nitrate reductase (NR) participates in salicylic acid (SA)-improved water stress (WS) tolerance in pepper (Capsicum annuum L.) plants. Before starting WS treatment, 0.5 mM SA was applied to half of the well-watered (WW) plants as well as to WS-plants as a foliar spray once a day for a week. The soil water holding capacity was maintained at 40 and 80% of the full water storing capacity for WS and and well-watered (WW) plants, respectively. Water stress caused substantial decreases in total plant dry weight, F v /F m , chlorophyll a and b, relative water content, leaf water potential (ΨI) by 53, 37, 49, 21, 36 and 33%, respectively relative to control, but significant increases in malondialdehyde (MDA), hydrogen peroxide (H 2 O 2 ), electrolyte leakage (EL), methylglyoxal (MG), proline, key antioxidant enzymes' activities, NO and NR activity. The SA reduced oxidative stress, but improved antioxidant defence system, ascorbate-glutathione (AsA-GSH) cycle enzymes, glyoxalase system-related enzymes, glyoxalase I (Gly I) and glyoxalase II (Gly II), plant growth, photosynthetic traits, NO, NR and proline. SA-induced WS tolerance was further improved by supplementation of sodium nitroprusside (SNP), a donor of NO. NR inhibitor, sodium tungstate (ST) was applied in conjunction with SA and SA + SNP to the WW and WS-plants to assess whether NR contributes to SA-improved WS tolerance. ST abolished the beneficial effects of SA by reducing NO and NR activity in WS-pepper, but the application of SNP along with SA + ST reversed negative effects of ST, showing that NO and NR are jointly needed for SA-induced WS tolerance of pepper plants.

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Section: Introductionmentioning

confidence: 99%

Nitrate reductase is required for salicylic acid‐induced water stress tolerance of pepper by upraising the AsA‐GSH pathway and glyoxalase system

Kaya

2020

Physiologia Plantarum

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“…(1995), measured as the electrical conductivity of a solution obtained after the incubation of roots in DDW (Khan et al . 2020b). Lipid peroxidation was assessed by estimating the concentration of thiobarbituric acid reactive substances (TBARS) according to the method of Cakmak & Horst (1991) as explained by Khan et al .…”

Section: Methodsmentioning

confidence: 99%

Exogenous melatonin‐mediated regulation of K⁺/Na⁺ transport, H⁺‐ATPase activity and enzymatic antioxidative defence operate through endogenous hydrogen sulphide signalling in NaCl‐stressed tomato seedling roots

et al. 2021

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Melatonin (Mel) and hydrogen sulphide (H2S) have emerged as potential regulators of plant metabolism during abiotic stress. Presence of excess NaCl in the soil is one of the main causes of reduced crop productivity worldwide. The present investigation examines the role of exogenous Mel and endogenous H2S in tomato seedlings grown under NaCl stress. Effect of 30 µm Mel on endogenous synthesis of H2S was examined in roots of NaCl‐stressed (200 mm) tomato seedlings. Also, the impact of treatments on the oxidative stress markers, transport of K+ and Na+, and activity of H+‐ATPase and antioxidant enzymes was assessed. Results show that NaCl‐stressed seedlings supplemented with 30 µm Mel had increased levels of endogenous H2S through enhanced L‐cysteine desulfhydrase activity. Mel in association with H2S overcame the deleterious effect of NaCl and induced retention of K+ that maintained a higher K+/Na+ ratio. Use of plasma membrane inhibitors and an H2S scavenger revealed that Mel‐induced regulation of K+/Na+ homeostasis in NaCl‐stressed seedling roots operates through endogenous H2S signalling. Synergistic effects of Mel and H2S also reduced the generation of ROS and oxidative destruction through the enhanced activity of antioxidant enzymes. Thus, it is suggested that the protective function of Mel against NaCl stress operates through an endogenous H2S‐dependent pathway, wherein H+‐ATPase‐energized secondary active transport regulates K+/Na+ homeostasis.

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“…The effect of treatments on oxidative stress-induced damage was evaluated by estimating lipid peroxidation by measuring thiobarbituric acid reactive substances (TBARSs) according to Cakmak and Horst [ 81 ]. Relative water content (RWC) was measured according to Yamasaki and Dillenburg [ 82 ] using fresh weight, dry weight, and turgid weight of the roots [ 83 ]. Electrolyte leakage (ELKG) was determined according to Lutts et al [ 84 ].…”

Section: Methodsmentioning

confidence: 99%

“…Electrolyte leakage (ELKG) was determined according to Lutts et al [ 84 ]. The detailed method is described by Khan et al [ 83 ].…”

Section: Methodsmentioning

confidence: 99%

Exogenous Potassium (K+) Positively Regulates Na+/H+ Antiport System, Carbohydrate Metabolism, and Ascorbate–Glutathione Cycle in H2S-Dependent Manner in NaCl-Stressed Tomato Seedling Roots

Khan

Bhatla

Al-Huqail

et al. 2021

Plants

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Potassium (K+) is one of the vital macronutrients required by plants for proper growth and blossoming harvest. In addition, K+ also plays a decisive role in promoting tolerance to various stresses. Under stressful conditions, plants deploy their defense system through various signaling molecules, including hydrogen sulfide (H2S). The present investigation was carried out to unravel the role of K+ and H2S in plants under NaCl stress. The results of the study show that NaCl stress caused a reduction in K+ and an increase in Na+ content in the tomato seedling roots which coincided with a lower H+-ATPase activity and K+/Na+ ratio. However, application of 5 mM K+, in association with endogenous H2S, positively regulated the Na+/H+ antiport system that accelerated K+ influx and Na+ efflux, resulting in the maintenance of a higher K+/Na+ ratio. The role of K+ and H2S in the regulation of the Na+/H+ antiport system was validated by applying sodium orthovanadate (plasma membrane H+-ATPase inhibitor), tetraethylammonium chloride (K+ channel blocker), amiloride (Na+/H+ antiporter inhibitor), and hypotaurine (HT, H2S scavenger). Application of 5 mM K+ positively regulated the ascorbate–glutathione cycle and activity of antioxidant enzymes that resulted in a reduction in reactive oxygen species generation and associated damage. Under NaCl stress, K+ also activated carbohydrate metabolism and proline accumulation that caused improvement in osmotic tolerance and enhanced the hydration level of the stressed seedlings. However, inclusion of the H2S scavenger HT reversed the effect of K+, suggesting H2S-dependent functioning of K+ under NaCl stress. Therefore, the present findings report that K+, in association with H2S, alleviates NaCl-induced impairments by regulating the Na+/H+ antiport system, carbohydrate metabolism, and antioxidative defense system.

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Nitric oxide is involved in nano-titanium dioxide-induced activation of antioxidant defense system and accumulation of osmolytes under water-deficit stress in Vicia faba L.

Cited by 86 publications

References 96 publications

Nitrate reductase is required for salicylic acid‐induced water stress tolerance of pepper by upraising the AsA‐GSH pathway and glyoxalase system

Nitrate reductase is required for salicylic acid‐induced water stress tolerance of pepper by upraising the AsA‐GSH pathway and glyoxalase system

Exogenous melatonin‐mediated regulation of K⁺/Na⁺ transport, H⁺‐ATPase activity and enzymatic antioxidative defence operate through endogenous hydrogen sulphide signalling in NaCl‐stressed tomato seedling roots

Exogenous Potassium (K+) Positively Regulates Na+/H+ Antiport System, Carbohydrate Metabolism, and Ascorbate–Glutathione Cycle in H2S-Dependent Manner in NaCl-Stressed Tomato Seedling Roots

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Nitric oxide is involved in nano-titanium dioxide-induced activation of antioxidant defense system and accumulation of osmolytes under water-deficit stress in Vicia faba L.

Cited by 86 publications

References 96 publications

Nitrate reductase is required for salicylic acid‐induced water stress tolerance of pepper by upraising the AsA‐GSH pathway and glyoxalase system

Nitrate reductase is required for salicylic acid‐induced water stress tolerance of pepper by upraising the AsA‐GSH pathway and glyoxalase system

Exogenous melatonin‐mediated regulation of K+/Na+ transport, H+‐ATPase activity and enzymatic antioxidative defence operate through endogenous hydrogen sulphide signalling in NaCl‐stressed tomato seedling roots

Exogenous Potassium (K+) Positively Regulates Na+/H+ Antiport System, Carbohydrate Metabolism, and Ascorbate–Glutathione Cycle in H2S-Dependent Manner in NaCl-Stressed Tomato Seedling Roots

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Exogenous melatonin‐mediated regulation of K⁺/Na⁺ transport, H⁺‐ATPase activity and enzymatic antioxidative defence operate through endogenous hydrogen sulphide signalling in NaCl‐stressed tomato seedling roots