Nitric oxide-mediated injury of interstitial cells of Cajal and intestinal dysmotility under endotoxemia of mice

Ueshima, Shigeyuki; Nishida, Toshirou; Koike, Masato; Matsuda, Hikaru; Sawa, Yoshiki; Uchiyama, Yasuo

doi:10.2220/biomedres.35.251

Cited by 8 publications

(7 citation statements)

References 31 publications

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“…The myoelectrical activity and intraluminal pressure of the intestine were recorded as described previously [19, 22, 23]. The mice were anesthetized with amobarbital sodium.…”

Section: Methodsmentioning

confidence: 99%

Targeting IL-17A Improves the Dysmotility of the Small Intestine and Alleviates the Injury of the Interstitial Cells of Cajal during Sepsis

Kong

Chen

et al. 2019

Oxidative Medicine and Cellular Longevity

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Intestinal dysmotility is a frequent complication during sepsis and plays an important role in the development of secondary infections and multiple organ failure. However, the central mechanisms underlying this process have not been well elucidated. Currently, effective therapies are still lacking for the treatment of sepsis-induced intestinal dysmotility. In this study, we found that the activation of IL-17 signaling within the muscularis propria might be associated with dysmotility of the small intestine during polymicrobial sepsis. Furthermore, we demonstrated that targeting IL-17A partially rescued the motility of the small intestine and alleviated interstitial cells of Cajal (ICC) injury during sepsis. The blockade of IL-17A suppressed the dominant sepsis-induced infiltration of M1-polarized macrophages into the muscularis. Additionally, impaired ICC survival may be associated with the oxidative stress injury induced by dominant infiltration of M1-polarized macrophages. Our findings reveal the important role of the IL-17 signaling pathway in the small intestine during sepsis and provide clues for developing a novel therapeutic strategy for treating gastrointestinal dysmotility during sepsis.

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“…The myoelectrical activity and intraluminal pressure of the intestine were recorded as described previously [19, 22, 23]. The mice were anesthetized with amobarbital sodium.…”

Section: Methodsmentioning

confidence: 99%

Targeting IL-17A Improves the Dysmotility of the Small Intestine and Alleviates the Injury of the Interstitial Cells of Cajal during Sepsis

Kong

Chen

et al. 2019

Oxidative Medicine and Cellular Longevity

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“…ICC generate spontaneous electrical slow waves and regulate rhythmic peristalsis. Loss of ICC typically causes gastrointestinal dysmotility [ 13 ]. Increasing evidence suggests that ICC depletion and damage, network disruption and channel apathies may lead to aberrant slow wave initiation and conduction [ 14 ].…”

Section: Introductionmentioning

confidence: 99%

Effects of Electroacupuncture on Interstitial Cells of Cajal (ICC) Ultrastructure and Connexin 43 Protein Expression in the Gastrointestinal Tract of Functional Dyspepsia (FD) Rats

Zhang

Shen

et al. 2016

Med Sci Monit

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BackgroundGastrointestinal motility disorder is the main clinical manifestation in functional dyspepsia (FD) patients. Electroacupuncture is effective in improving gastrointestinal motility disorder in FD; however, the underlying mechanism remains unclear. It has been demonstrated that interstitial cells of Cajal (ICC) are pacemaker cells in the gastrointestinal tract, and the pacemaker potential is transmitted to nearby cells through gap junctions between ICC or ICC and the smooth muscle. Therefore, this study aimed to assess the effects of electroacupuncture on ICC ultrastructure and expression of the gap junction protein connexin 43 (Cx43) in FD rats.Material/MethodsThe animals were randomized into 3 groups: control, model, and electroacupuncture. Electroacupuncture was applied at Zusanli (ST36) in the electroacupuncture group daily for 10 days, while no electroacupuncture was applied to model group animals.ResultsUltrastructure of ICC recovered normally in gastric antrum and small intestine specimens was improved, with Cx43 expression levels in these tissues significantly increased in the electroacupuncture group compared with the model group.ConclusionsThese findings indicated that electroacupuncture is effective in alleviating ICC damage and reduces Cx43 levels in FD rats, and suggest that ICC and Cx43 are involved in electroacupuncture treatment in rats with FD to improve gastrointestinal motility disorders.

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“…Furthermore, NO donor treatment disrupted the networks of ICC in cultured smooth muscle tissue. Some reports also suggest the negative effect of NO derived from iNOS on the networks of ICC in experimental models of sepsis 43 and surgical resection. 18 These results and reports strongly suggest the participation of NO in the ICC dysfunction during IM-induced inflammation.…”

Section: Im-induced Nitric Oxide Disrupts Icc Networkmentioning

confidence: 99%

Disruption of the pacemaker activity of interstitial cells of Cajal via nitric oxide contributes to postoperative ileus

Kaji

Nakayama

Horiguchi

et al. 2018

Neurogastroenterology Motil

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Nitric oxide-mediated injury of interstitial cells of Cajal and intestinal dysmotility under endotoxemia of mice

Cited by 8 publications

References 31 publications

Targeting IL-17A Improves the Dysmotility of the Small Intestine and Alleviates the Injury of the Interstitial Cells of Cajal during Sepsis

Targeting IL-17A Improves the Dysmotility of the Small Intestine and Alleviates the Injury of the Interstitial Cells of Cajal during Sepsis

Effects of Electroacupuncture on Interstitial Cells of Cajal (ICC) Ultrastructure and Connexin 43 Protein Expression in the Gastrointestinal Tract of Functional Dyspepsia (FD) Rats

Disruption of the pacemaker activity of interstitial cells of Cajal via nitric oxide contributes to postoperative ileus

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