Nitric Oxide-Mediated Transcriptional Changes in Arabidopsis thaliana

Abstract

Nitric oxide (NO) is an essential regulatory molecule in several developmental processes and in the stress response in both animal and plant systems. Furthermore, key features of plant resistance to pathogens have been shown to depend on NO production, e.g., defense gene expression and the activation of a hypersensitive reaction (HR) in synergy with reactive oxygen species (ROS). Due to the many possible mechanisms of NO action, a clear picture of its involvement in plant resistance to pathogens is far from being achieved. Transcriptional changes related to NO action are likely to play a significant role in resistance and cell death. We investigated the changes in the expression profiles of Arabidopsis thaliana following infiltration with the NO donor sodium nitroprusside, by cDNA-amplification fragment length polymorphism (AFLP) transcript profiling. Altered expression patterns were detected for 120 of the approximately 2,500 cDNAs examined. Sequence analysis revealed homologies with genes involved in signal transduction, disease resistance and stress response, photosynthesis, cellular transport, and basic metabolism or with sequences coding for unknown proteins. Comparison of the expression profiles with data from public microarray sources revealed that many of the identified genes modulated by NO were previously reported to be modulated in disease-related experiments.

Nitric Oxide and ProteinS-Nitrosylation Are Integral to Hydrogen Peroxide-Induced Leaf Cell Death in Rice

“…It has been shown that NO regulates a number of physiological processes directly by affecting gene transcription (Huang et al, 2002;Wang et al, 2002;Polverari et al, 2003;Parani et al, 2004;Shoulars et al, 2008). NO can also regulate specific physiological and developmental processes through its interplay with other small biomolecules.…”

Nuclear Accumulation of Cytosolic Glyceraldehyde-3-Phosphate Dehydrogenase in Cadmium-Stressed Arabidopsis Roots

“…Not surprisingly, several proteomic studies have recently reported that H 2 O 2 , NO-donors, and biotic and abiotic stresses applied to plants may affect the redox state of reactive protein cysteines, in addition to affecting their transcriptomic and proteomic profiles Polverari et al, 2003;Parani et al, 2004;Lindermayr et al, 2005;Herbette et al, 2006;Roth et al, 2006;Sarry et al, 2006;Besson-Bard et al, 2008;Romero-Puertas et al, 2008;Astier et al, 2011). Because of the high reactivity of its catalytic Cys, cytosolic glyceraldehyde 3-P dehydrogenase (GAPDH) has been identified as a target of different types of redox modifications (Hancock et al, 2005(Hancock et al, , 2006Lindermayr et al, 2005;Holtgrefe et al, 2008;Bedhomme et al, 2012).…”

NO serves as a signaling intermediate downstream of H2O2to modulate dynamic microtubule cytoskeleton during responses to VD-toxins in Arabidopsis

“…[5][6][7][8] It has been demonstrated that NO cooperates with H 2 O 2 to activate the hypersensitive reaction in plants. [9][10][11][12] However, the interaction of NO and H 2 O 2 is still far from being clearly elucidated. [13][14][15][16][17] We have recently demonstrated that NO and H 2 O 2 can act as an upstream signaling molecule to modulate the dynamic microtubule cytoskeleton during the defense responses to Verticillium dahliae (VD) toxins in Arabidopsis.…”