Nitric Oxide Mediates Acute Lung Injury by Modulation of Inflammation

Tavaf-Motamen, Houman; Miner, Thomas J.; Starnes, Benjamin W.; Shea‐Donohue, Terez

doi:10.1006/jsre.1998.5395

Cited by 57 publications

(37 citation statements)

References 27 publications

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“…It may be that there was a transient injury to the pulmonary endothelium with leakage of albumin into the surrounding tissues, but 12 h after the end of ischemia a persisting increase in albumin flux over the endothelial barrier was not demonstrable. Our results are supported by data from Tavaf-Motamen et al [28]who in a model of 30 min intestinal ischemia show a peak in pulmonary capillary leak 4 h after the onset of reperfusion, with return to baseline after 12 h reperfusion. It is possible that alterations in pulmonary endothelial barrier integrity may result from endothelial functional responses (e.g.…”

Section: Discussionsupporting

confidence: 82%

Effects of N-Acetylcysteine on Pulmonary Macrophage Activity after Intestinal Ischemia and Reperfusion in Rats / with Invited Commentaries

et al. 2000

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Background/Aims: Intestinal ischemia and reperfusion (I/R) is considered to be a critical and triggering event in the development of distal organ dysfunction after a variety of insults. It appears that activated leukocytes, especially polymorphonuclear granulocytes (PMNs), and reactive oxygen species are important mediators in the process. In the present study, the aim was to evaluate the behavior of pulmonary macrophages, acute lung injury and pulmonary endothelial permeability after intestinal I/R, together with potential alterations in pulmonary endothelial and epithelial ultrastructure and cellular membrane system integrity. Methods: Intestinal ischemia for 40 min was followed by reperfusion for 12 h in the rat. Macrophage uptake of radiolabeled bacteria, levels of pulmonary blood content assessed by radiolabeled red blood cells and pulmonary endothelial permeability of radiolabeled albumin, as well as pulmonary endothelial and epithelial ultrastructure and cellular membrane system integrity by the use of scanning electron microscopy and a tracer was evaluated after 12 h reperfusion. Treatment with the free radical scavenger N-acetylcysteine (NAC) administered prior to reperfusion was evaluated. Results: Overactivation of pulmonary macrophages was noted after intestinal I/R, as was a significant decrease in pulmonary blood content. No increase in pulmonary albumin leakage or increase in pulmonary water content was found after intestinal I/R as compared to controls. Treatment with NAC prevented against intestinal I/R-induced overactivation of pulmonary macrophages and a decrease in pulmonary blood content. Conclusion: Reactive oxygen species may be involved in the regulation of pulmonary macrophage function and pulmonary circulation after intestinal I/R.

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Section: Discussionsupporting

confidence: 82%

Effects of N-Acetylcysteine on Pulmonary Macrophage Activity after Intestinal Ischemia and Reperfusion in Rats / with Invited Commentaries

et al. 2000

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“…As the intestine normally receives more blood from the heart than any other organ with the splanchnic circulation consisting of up to 25-30% of the total blood volume, hemorrhage-induced decreased intestinal blood flow and associated vasoconstriction, may result in a functional intestinal ischemia (5). In other models of ischemia, this process leads to local and systemic damage and is suggested to be critical in the induction of multiple organ failure (6). Mechanisms of ischemia and subsequent reperfusion-induced intestinal mucosal injury include oxidative stress and excessive complement activation (7)(8)(9).…”

Section: Introductionmentioning

confidence: 99%

Complement Component C5a Mediates Hemorrhage-Induced Intestinal Damage

Fleming

Phillips

Lambris

et al. 2008

Journal of Surgical Research

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“…Akciğer ödeminin derecesini değerlendirmek amacıyla çeşitli metotlar kullanılmaktadır (5)(6)(7)(8). Çalışmamızda akciğer vasküler yatağında mikrovasküler permeabilite deği-şikliklerini değerlendirmek amacıyla akciğer dokusu yaş/kuru ağırlığı oranı kullanmıştır.…”

Section: Discussionunclassified

Farklı Lavaj Solüsyonlarının Akut Akciğer Hasarı Oluşumundaki Etkilerinin Araştırılması

Gündoğdu¹,

Özcan²,

Telci³

et al. 2011

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Amaç: Çeşitli çalışmalarda Ringer laktat solüsyonunun izotonik salin (%0,9 NaCl) solüsyona oranla üst hava yollarında, mukosiliyer aktivite ve klirens üzerinde koruyucu etkilerinin olduğu göste-rilmiştir. Çalışmamızda canlı dokularda irrigasyon için daha uygun olarak gösterilen Ringer laktat solüsyonunun izotonik salin solüs-yona göre akciğer hasarına etkisini araştırmayı hedefledik. Gereç ve Yöntem: İstanbul Üniversitesi, İstanbul Tıp Fakültesi Deneysel Hayvan Araştırmaları Enstitüsü Etik Kurulu onayı alın-dıktan sonra toplam 24 adet 250-300 g ağırlığında SpragueDawley türü sıçan anestezi altında trakeostomize edilmiştir. Sıçanlar basınç kontrollü ventilasyon modunda ventile edilmiştir. Çalışmamızın ilk bölümünde Ringer laktat ve izotonik salin solüs-yonları ile ARDS oluşturan lavaj miktarının bulunması amaçlan-mıştır. Çalışmamızın ikinci bölümünde erken ARDS oluşturduğu belirlenen miktarda sıvı kullanılarak iki farklı gruba ayrılan deneklerde inceleme yapılmıştır. Deneklerin akciğer hasarı akciğer yaş/kuru ağırlıkları, BAL'da mikroalbümin düzeyi ve arter kan gazı değişiklikleri ile değerlendirilmiştir. Bulgular: Beklediğimizin aksine izotonik salin ile ortalama 11,4 adet lavajla ARDS oluşurken Ringer laktat ile 10,57 adet lavajla ARDS gelişmiştir. Çalışmamızın ikinci bölümünde her iki solüsyon-la 10'ar adet lavaj uygulanmış, yaş/kuru ağırlık ve BAL'da miroalbümin düzeyleri istatistiksel olarak anlamlı olmasa da izotonik lavaj grubunda düşük bulunmuştur. ÖZET SUMMARYAim: Experimental animal studies showed that lactated Ringer solution have beneficial effects over isotonic saline solution (0.9% NaCl) for mucociliar activity and clearance of upper respiratory tract. In this study we evaluated the effect of lactated Ringer solution and isotonic saline solution on rat lungs for lung injury. Material and Method: Istanbul University, Istanbul Medical Faculty, Experimental Animal Researches Study Institute ethical committee approval was obtained for 24 Sprague-Dawley rats each weighing between 250-300 g. Rats were tracheostomized under general anesthesia and ventilated using pressure controlled ventilation mode. As the first part of our study, we aimed to find the adequate lavage volume of isotonic saline and lactated Ringer solutions to induce ARDS. After finding the adequate lavage volume and count; the mean lavage count that induce ARDS, rest of the rats were randomly divided into two groups and tracheal lavages were performed according to predetermined lavage volume and count in the second part of the study. Wet/dry body weight counts, arterial blood gas sampling and microalbumin levels of bronchoalveolar lavage were analyzed for assessment of lung injury. Results: ARDS was developed following 11.4 lavages with isotonic saline solution and 10.57 lavages with lactated Ringer solutions. In the second part of the study, wet/dry body weight and BAL microalbumin levels were found lower in isotonic saline group however the difference between groups were not statistically significant. Conclusion:We were not able to demo...

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Nitric Oxide Mediates Acute Lung Injury by Modulation of Inflammation

Cited by 57 publications

References 27 publications

Effects of N-Acetylcysteine on Pulmonary Macrophage Activity after Intestinal Ischemia and Reperfusion in Rats / with Invited Commentaries

Effects of N-Acetylcysteine on Pulmonary Macrophage Activity after Intestinal Ischemia and Reperfusion in Rats / with Invited Commentaries

Complement Component C5a Mediates Hemorrhage-Induced Intestinal Damage

Farklı Lavaj Solüsyonlarının Akut Akciğer Hasarı Oluşumundaki Etkilerinin Araştırılması

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