Nitric oxide metabolites in naturally occurring canine babesiosis

Jacobson, Linda S.; Lobetti, Remo; Becker, P J; Reyers, Fred; Vaughan-Scott, T.

doi:10.1016/s0304-4017(01)00606-9

Cited by 27 publications

(25 citation statements)

References 55 publications

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“…Numerous studies on the immune response of Th1 cells, characterized by the synthesis of proinflammatory cytokines and iNOS, have been performed in different hemoparasites [13-15]. However, the mechanism of host immune responses has not been explained in R. vitalii infection.…”

mentioning

confidence: 99%

Increased Cytokine and Nitric Oxide Levels in Serum of Dogs Experimentally Infected with Rangelia vitalii

et al. 2013

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This study aimed to measure the levels of interferon-gamma (IFN-γ), tumor necrosis factor-alpha (TNF-α), interleukin 1 (IL-1), interleukin 6 (IL-6), and nitrite/nitrate (NOx) in serum of dogs experimentally infected with Rangelia vitalii. Twelve female mongrel dogs were divided into 2 groups; group A (uninfected controls) composed by healthy dogs (n=5) and group B consisting of dogs inoculated with R. vitalii (n=7). Animals were monitored by blood smear examinations, which showed intraerythrocytic forms of the parasite on day 5 post-infection (PI). Blood samples were collected through the jugular vein on days 0, 10, and 20 PI to determine the serum levels of IFN-γ, TNF-α, IL-1, IL-6, and NOx. Cytokines were assessed by ELISA quantitative sandwich technique, and NOx was measured by the modified Griess method. Cytokine levels (IFN-γ, TNF-α, IL-1, and IL-6) were increased (P<0.01) in serum of infected animals. Serum levels of NOx were also increased on days 10 PI (P<0.01) and 20 PI (P<0.05) in infected animals. Therefore, the infection with R. vitalii causes an increase in proinflammatory cytokines and nitric oxide content. These alterations may be associated with host immune protection against the parasite.

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confidence: 99%

Increased Cytokine and Nitric Oxide Levels in Serum of Dogs Experimentally Infected with Rangelia vitalii

et al. 2013

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“…Reactive nitrogen intermediates (RNI), nitrate and nitrite serve as surrogate measures for NO (46). With the exception of a few complicated Babesia-infected dogs, RNI did not differ significantly between babesiosis cases and healthy control dogs (46). However, one study did report that RNI were significantly higher in dogs with babesiosis compared to tick-free healthy laboratory control dogs (46).…”

Section: Immunopathology In Babesiosismentioning

confidence: 93%

“…With the exception of a few complicated Babesia-infected dogs, RNI did not differ significantly between babesiosis cases and healthy control dogs (46). However, one study did report that RNI were significantly higher in dogs with babesiosis compared to tick-free healthy laboratory control dogs (46). The presence of an acute phase response is undeniable in canine babesiosis (41,47,48).…”

Section: Immunopathology In Babesiosismentioning

confidence: 99%

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A flow cytometric assessment of the lymphocyte immunophenotypes in dogs naturally infected with Babesia rossi

Rautenbach

Goddard

Thompson

et al. 2017

Veterinary Parasitology

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“…W 2014 r. opublikowano wyniki przeprowadzonych na WMW SGGW badań, w których po raz pierwszy na świecie stwierdzono wpływ zwiększonej produkcji prozapalnej cytokiny TNF-α na spadek ciśnienia tęt-niczego krwi i uszkodzenie nerek u psów zarażonych pierwotniakami z rodzaju Babesia (71). Na podstawie uzyskanych wyników oraz wcześniejszych badań naukowców z Węgier i RPA, gdzie stwierdzono typowe dla niedotlenienia nerek zmiany martwicze w kanalikach bliższych u psów zarażonych B. canis (41) oraz wzrost stężenia metabolitów tlenku azotu we krwi psów zarażonych B. rossi (28), postawiono hipotezę, według której zwiększona produkcja TNF-α prowadzi do zwiększenia produkcji tlenku azotu powodującego rozszerzenie naczyń krwionośnych, a w konsekwencji obniżenie ciśnienia tętniczego, co z kolei skutkuje niedokrwieniem i niedotlenieniem nerek, prowadząc do ich ostrej niewydolności u zarażonych zwierząt (71).…”

Section: Over 20 Years Of Research On Canine Babesiosis At the Facultunclassified

Over 20 years of research on canine babesiosis at the Faculty of Veterinary Medicine of WULS-SGGW in Warsaw

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Gójska-Zygner

Górski

et al. 2017

Medycyna Weterynaryjna

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Canine babesiosis is a tick-borne disease caused by infection with protozoa of the genus Babesia. During over 20 years of studies on canine babesiosis at the Faculty of Veterinary Medicine of the Warsaw University of Life Sciences, the researchers have identified the species of the parasite and its vector in Poland, determined the cause of azotemia and observed other pathological changes, such as endocrine disorders, changes in the de Ritis quotient, as well as biochemical and hematological changes. It was shown that the tick Dermacentor reticulatus is the only vector of canine babesiosis in Poland and the disease is caused by Babesia canis (formerly known as Babesia canis canis). The first studies of pathological changes confirmed the results of previous research in other countries, in which the most prevalent changes in canine babesiosis were thrombocytopenia, anemia leucopenia, increased activity of liver enzymes (ALT, AST, ALP), azotemia, hypoalbuminemia, hypoglycemia, hyponatremia and hypokalemia. Research on azotemia in dogs infected with B. canis showed the contribution of TNF-α overproduction to hypotension and its influence on renal ischemia, hypoxia and the development of azotemia. Moreover, in research on endocrine disorders in canine babesiosis, it was shown that IL-6 overproduction leads to the suppression of the hypothalamic-pituitary-thyroid axis resulting in euthyroid sick syndrome, and that secondary hyperaldosteronism results from decreased renal blood flow and leads to hypokalemia. The results of the research were published in 27 original papers, 6 case reports and many review articles in veterinary journals. These studies provided veterinary surgeons in Poland with improved procedures for diagnosing canine babesiosis, as well as allowed them to better understand some pathogenic mechanisms of the disease and to develop more efficacious therapies..

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Nitric oxide metabolites in naturally occurring canine babesiosis

Cited by 27 publications

References 55 publications

Increased Cytokine and Nitric Oxide Levels in Serum of Dogs Experimentally Infected with Rangelia vitalii

Increased Cytokine and Nitric Oxide Levels in Serum of Dogs Experimentally Infected with Rangelia vitalii

A flow cytometric assessment of the lymphocyte immunophenotypes in dogs naturally infected with Babesia rossi

Over 20 years of research on canine babesiosis at the Faculty of Veterinary Medicine of WULS-SGGW in Warsaw

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