Nitric Oxide Modulates Postnatal Bone Marrow-Derived Mesenchymal Stem Cell Migration

Fuseler, John W.; Valarmathi, Mani T.

doi:10.3389/fcell.2016.00133

Cited by 7 publications

(7 citation statements)

References 74 publications

(131 reference statements)

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“…The FD measurement was shown to be as a useful tool to estimate the organization of cellular F-actin in several studies [ 8 , 14 ]. However, this method has not become widely used in biological research, although the number of publications that mention FD is growing.…”

Section: Discussionmentioning

confidence: 99%

“…Transplantation of mesenchymal stem cells is a promising tool for regenerative medicine, but most stem cell-based therapies are currently at different stages of clinical research because of a number of various restrictions including the notion that relatively low percentage of cells successfully reaches the injured area [ 12 , 13 ]. At this time, specific protocols such as co-cultivation and preconditioning of stem cells with biologically active compounds including those that influence microfilament organization, are being used as a potential approach to improve their regeneration properties [ 14 , 15 ].…”

Section: Introductionmentioning

confidence: 99%

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The analysis of F-actin structure of mesenchymal stem cells by quantification of fractal dimension

et al. 2021

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The actin cytoskeleton is indispensable for the motility and migration of all types of cells; therefore, it plays a crucial role in the ability of the tissues to repair. Mesenchymal stem cells are intensively used in regenerative medicine, but usually relatively low percent of transplanted cells reaches the injury. To overcome this evident limitation, researchers try to enhance the motility and migration rate of the cells. As one of the approaches, co-cultivation and preconditioning of stem cells with biologically active compounds, which can cause actin cytoskeleton rearrangements followed by an increase of migratory properties of the cells, could be applied. The observed changes in F-actin structure induced by the compounds require quantitative estimation, and measurement of fluorescence intensity of the F-actin image captured by various microscopic techniques is commonly used nowadays. However, this approach could not always accurately detect the observed changes in the shape and structure of actin cytoskeleton. At this time, the image of F-actin has an irregular geometric pattern, and thus could be considered and characterized as a fractal object. To quantify the re-organization of cellular F-actin in terms of fractal geometry Minkovsky’s box-counting method is suitable, but it is not widely used nowadays. We modified and improved the previously described method for fractal dimension measurement, and successfully applied it for the quantification of the F-actin structures of human mesenchymal stem cells.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The analysis of F-actin structure of mesenchymal stem cells by quantification of fractal dimension

et al. 2021

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“…NO, an inflammatory molecule, promotes immunocytes to migrate to lesions by dispersing through the microenvironment. NO either stimulates or inhibits cellular migration, depending on the conditions, tissue location, and types of cells present (Fuseler et al, 2016). NO has been shown to be either a requirement or a stimulus for cellular migration.…”

Section: Discussionmentioning

confidence: 99%

Human endogenous retrovirus W env increases nitric oxide production and enhances the migration ability of microglia by regulating the expression of inducible nitric oxide synthase

Xiao

Cao

et al. 2017

Virol. Sin.

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Human endogenous retrovirus W env (HERV-W env) plays a critical role in many neuropsychological diseases such as schizophrenia and multiple sclerosis (MS). These diseases are accompanied by immunological reactions in the central nervous system (CNS). Microglia are important immunocytes in brain inflammation that can produce a gasotransmitter-nitric oxide (NO). NO not only plays a role in the function of neuronal cells but also participates in the pathogenesis of various neuropsychological diseases. In this study, we reported increased NO production in CHME-5 microglia cells after they were transfected with HERV-W env. Moreover, HERV-W env increased the expression and function of human inducible nitric oxide synthase (hiNOS) and enhanced the promoter activity of hiNOS. Microglial migration was also enhanced. These data revealed that HERV-W env might contribute to increase NO production and microglial migration ability in neuropsychological disorders by regulating the expression of inducible NOS. Results from this study might lead to the identification of novel targets for the treatment of neuropsychological diseases, including neuroinflammatory diseases, stroke, and neurodegenerative diseases.

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“…[51] NO functions as a stopmigration signal by disordering the cytoskeletal elements required for cell movement and proliferation of MSCs. 52 Herein, we showcased the feasibility of our NO donor in modulating MSC migration. In the wound model of cellular migration, cells at the margin of the wound preferentially migrated into the cell-free zone (wound) without the addition of attractants.…”

Section: Introductionmentioning

confidence: 97%

“…Similar inhibitory results on cellular migration have been observed in MSCs and cancer cells that were treated with other NO donors such as SNAP. [52]…”

Section: Introductionmentioning

confidence: 99%

A Photo-triggered and photo-calibrated nitric oxide donor: Rational design, spectral characterizations, and biological applications

Liu²,

Zhou

et al. 2018

Free Radical Biology and Medicine

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Nitric oxide (NO) donors are valuable tools to probe the profound implications of NO in health and disease. The elusive nature of NO bio-relevance has largely limited the use of spontaneous NO donors and promoted the development of next generation NO donors, whose NO release is not only stimulated by a trigger, but also readily monitored via a judiciously built-in self-calibration mechanism. Light is without a doubt the most sensitive, versatile and biocompatible method of choice for both triggering and monitoring, for applications in complex biological matrices. Herein, we designed and synthesized an N-nitroso rhodamine derivative (NOD560) as a photo-triggered and photo-calibrated NO donor to address this need. NOD560 is essentially non-fluorescent. Upon irradiation by green light (532 nm), it efficiently release NO and a rhodamine dye, the dramatic fluorescence turn-on from which could be harnessed to conveniently monitor the localization, flux, and dose of NO release. The potentials of NOD560 for in vitro biological applications were also exemplified in in vitro biological models, i.e. mesenchymal stem cell (MSC) migration suppression. NOD560 is expected to complement the existing NO donors and find widespread applications in chemical biological studies.

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Nitric Oxide Modulates Postnatal Bone Marrow-Derived Mesenchymal Stem Cell Migration

Cited by 7 publications

References 74 publications

The analysis of F-actin structure of mesenchymal stem cells by quantification of fractal dimension

The analysis of F-actin structure of mesenchymal stem cells by quantification of fractal dimension

Human endogenous retrovirus W env increases nitric oxide production and enhances the migration ability of microglia by regulating the expression of inducible nitric oxide synthase

A Photo-triggered and photo-calibrated nitric oxide donor: Rational design, spectral characterizations, and biological applications

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