Nitric oxide (NO), citrulline–NO cycle enzymes, glutamine synthetase, and oxidative status in kainic acid–mediated excitotoxicity in rat brain

Swamy, Mummedy; Sirajudeen, K. N. S.; Govindasamy, Chandran

doi:10.1080/01480540903130641

Cited by 25 publications

(22 citation statements)

References 41 publications

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“…In this context the cellular damage seems to be directly correlated to cellular ROS concentration. Usually, cells are able to cope with ROS through different protective mechanisms that prevent the generation of reactive species, or remove them before they can fatally damage cells [25]. The main target of free radicals are lipids, nucleic acids, carbohydrates and proteins.…”

Section: Discussionmentioning

confidence: 99%

Protein modification as oxidative stress marker in follicular fluid from women with polycystic ovary syndrome: the effect of inositol and metformin

Piomboni

Focarelli

Capaldo

et al. 2014

J Assist Reprod Genet

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Purpose The purpose of this study was to evaluate the oxidative stress status (OS) of follicular fluid (FF) and the oocyte quality in women with polycystic ovary syndrome (PCOS) undergoing different ovarian stimulation protocols. Methods FF samples were collected after gonadotropin administration in association or not with metformin or D-chiro-inositol (DCI). OS status was then evaluated by checking the follicular fluid protein oxidation profile after specific labeling of aminoacidic free-SH groups, and two-dimensional electrophoresis followed by qualitative and semiquantitative analysis. Oocyte quality was assessed by international morphological criteria. Results Our data indicated that both treatments, even if to different extent, recovered a significantly high level of free-SH groups in FF proteins of PCOS women clearly indicating a decrease of OS level with respect to that found in FF samples from gonadotropins alone treated women. A higher number of good quality MII oocytes was also observed in DCI (P<0.05) or metformin (P<0.05) study groups in comparison to untreated control group. Conclusion A natural supplement and a drug both showed a statistically significant positive effect on follicular milieu by decreasing the oxidative damage on FF proteins, as well as in recovering good quality oocytes.

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Section: Discussionmentioning

confidence: 99%

Protein modification as oxidative stress marker in follicular fluid from women with polycystic ovary syndrome: the effect of inositol and metformin

Piomboni

Focarelli

Capaldo

et al. 2014

J Assist Reprod Genet

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“…Epileptic form activity was shown to cause excessive production of ROS/RNS, a factor believed to be involved in the mechanisms leading to neurodegeneration and cell death (Itoh and Watanabe, 2009). The increased production of NO and increased oxidative stress in kainic acid mediated excitotoxicity and epilepsy has been reported earlier (Swamy et al, 2009(Swamy et al, , 2011a.…”

Section: Discussionmentioning

confidence: 99%

“…It was earlier reported that GS becomes nitrated and inhibited during PTZ induced seizure model at repeated PTZ seizure induction, but there was no decrease in GS protein level (Bidmon et al, 2008). Our earlier studies demonstrated that increased production of NO, increased activity of NOS, decreased activity of GS and increased oxidative stress in KA mediated excitotoxicity (Swamy et al, 2009(Swamy et al, , 2011a. Therefore the present study was conducted to assess the neuroprotective effects of the bee product propolis , by estimating the glutamine synthetase activity, nitric oxide (NO), thiobarbituric acid reactive substances (TBARS) concentration and total antioxidant status (TAS) in cerebral cortex (CC), cerebellum (CB) and brain stem (BS) of rats supplemented with propolis and subjected to KA mediated excitotoxicity.…”

Section: Introductionmentioning

confidence: 99%

Restoration Of Glutamine Synthetase Activity, Nitric Oxide Levels And Amelioration Of Oxidative Stress By Propolis In Kainic Acid Mediated Excitotoxicity

Swamy

Norlina

Azman

et al. 2014

Afr. J. Trad. Compl. Alt. Med.

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Background: Propolis has been proposed to be protective on neurodegenerative disorders. To understand the neuroprotective effects of honeybee propolis, glutamine synthetase (GS) activity, nitric oxide (NO), thiobarbituric acid reactive substances (TBARS) and total antioxidant status (TAS) were studied in different brain regions-cerebral cortex (CC), cerebellum (CB) and brain stem (BS) of rats supplemented with propolis and subjected to kainic acid (KA) mediated excitotoxicity. Materials and Methods: Male Sprague-Dawley rats were divided into four groups; Control group and KA group received vehicle and saline. Propolis group and propolis + KA group were orally administered with propolis (150mg/kg body weight), five times every 12 hours. KA group and propolis + KA group were injected subcutaneously with kainic acid (15mg/kg body weight) and were sacrificed after 2 hrs and CC, CB and BS were separated homogenized and used for estimation of GS activity, NO, TBARS, and TAS concentrations by colorimetric methods. Results were analyzed by oneway ANOVA, reported as mean + SD from 6 animals, and p<0.05 considered statistically significant. Results: NO was increased (p< 0.001) and GS activity was decreased (p< 0.001) in KA treated group compared to control group as well as propolis + KA treated group. TBARS was decreased and TAS was increased (p< 0.001) in propolis + KA treated group compared KA treated group. Conclusion:This study clearly demonstrated the restoration of GS activity, NO levels and decreased oxidative stress by propolis in kainic acid mediated excitotoxicity. Hence the propolis can be a possible potential candidate (protective agent) against excitotoxicity and neurodegenerative disorders.

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“…Propolis has been shown to have broad biological activities, which are principally attributed to the presence of flavonoids (major component; rutin, quercetin, galangin, etc. ), phenolic compounds and CAPE (Isla et Our earlier studies have shown that NOS and NO were increased along with increased oxidative stress markers in KA mediated excitotoxicity and epilepsy rat models (Swamy, et al, 2009(Swamy, et al, , 2011a. We have also reported the amelioration of oxidative stress and NO concentration by propolis suplimentation in KA mediated excitotoxicity .…”

Section: Swamy Et Al Afr J Tradit Complement Altern Med (2014) 11(5)mentioning

confidence: 99%

Propolis Ameliorates Tumor Nerosis Factor-α, Nitric Oxide levels, Caspase-3 and Nitric Oxide Synthase Activities in Kainic Acid Mediated Excitotoxicity in Rat Brain

Swamy¹,

Suhaili²,

Sirajudeen³

et al. 2014

Afr. J. Trad. Compl. Alt. Med.

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Background: Increased nitric oxide (NO), neuronal inflammation and apoptosis have been proposed to be involved in excitotoxicity plays a part in many neurodegenerative diseases. To understand the neuro-protective effects of propolis, activities of Nitric oxide synthase (NOS) and caspase-3 along with NO and tumor necrosis factor-α (TNF-α) levels were studied in cerebral cortex (CC), cerebellum (CB) and brain stem (BS) in rats supplemented with propolis prior to excitotoxic injury with kainic acid (KA). Materials and methods: Male Sprague-Dawley rats were divided into four groups (n=6 rats per group) as Control, KA, Propolis and KA+Propolis. The control group and KA group have received vehicle and saline. Propolis group and propolis + KA group were orally administered with propolis (150mg/kg body weight), five times every 12 hours. KA group and propolis +KA group were injected subcutaneously with kainic acid (15mg/kg body weight) and were sacrificed after 2 hrs. CC, CB and BS were separated, homogenized and used for estimation of NOS, caspase-3, NO and TNF-α by commercial kits. Results were analyzed by one way ANOVA, reported as mean + SD (n=6 rats), and p<0.05 was considered statistically significant. Results: The concentration of NO, TNF-α, NOS and caspase-3 activity were increased significantly (p<0.001) in all the three brain regions tested in KA group compared to the control. Propolis supplementation significantly (p<0.001) prevented the increase in NOS, NO, TNF-α and caspase-3 due to KA. Conclusion:Results of this study clearly demonstrated that the propolis supplementation attenuated the NOS, caspase-3 activities, NO, and TNF-α concentration and in KA mediated excitotoxicity. Hence propolis can be a possible potential protective agent against excitotoxicity and neurodegenerative disorders.

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Nitric oxide (NO), citrulline–NO cycle enzymes, glutamine synthetase, and oxidative status in kainic acid–mediated excitotoxicity in rat brain

Cited by 25 publications

References 41 publications

Protein modification as oxidative stress marker in follicular fluid from women with polycystic ovary syndrome: the effect of inositol and metformin

Protein modification as oxidative stress marker in follicular fluid from women with polycystic ovary syndrome: the effect of inositol and metformin

Restoration Of Glutamine Synthetase Activity, Nitric Oxide Levels And Amelioration Of Oxidative Stress By Propolis In Kainic Acid Mediated Excitotoxicity

Propolis Ameliorates Tumor Nerosis Factor-α, Nitric Oxide levels, Caspase-3 and Nitric Oxide Synthase Activities in Kainic Acid Mediated Excitotoxicity in Rat Brain

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