Nitric Oxide Pretreatment Enhances Atheroma Component Highlighting in Vivo with Intercellular Adhesion Molecule-1-Targeted Echogenic Liposomes

Kee, Patrick; Kim, Hyunggun; Huang, Shaoling; Laing, Susan T.; Moody, Melanie R.; Vela, Deborah; Klegerman, Melvin E.; McPherson, David D.

doi:10.1016/j.ultrasmedbio.2013.12.013

Cited by 15 publications

(12 citation statements)

References 52 publications

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“…The goal of these methods is to exploit changes in echogenecity 11 or speckle decorrelation 12 to detect the vasa vasorum. Using these linear approaches, researchers have visualized extraluminal contrast enhancement, [11][12][13] and performed molecular IVUS imaging. 14,15 However, low detection sensitivity and motion artifacts limit the performance of linear CE-IVUS.…”

Section: Introductionmentioning

confidence: 99%

Chirp-Coded Ultraharmonic Imaging with a Modified Clinical Intravascular Ultrasound System

et al. 2016

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Imaging plaque microvasculature with contrast-enhanced intravascular ultrasound (IVUS) could help clinicians evaluate atherosclerosis and guide therapeutic interventions. In this study, we evaluated the performance of chirp-coded ultraharmonic imaging using a modified IVUS system (iLab™, Boston Scientific/Scimed) equipped with clinically available peripheral and coronary imaging catheters. Flow phantoms perfused with a phospholipid-encapsulated contrast agent were visualized using ultraharmonic imaging at 12 MHz and 30 MHz transmit frequencies. Flow channels with diameters as small as 0.8 mm and 0.5 mm were visualized using the peripheral and coronary imaging catheters. Radio-frequency signals were acquired at standard IVUS rotation speed, which resulted in a frame rate of 30 frames/s. Contrast-to-tissue ratios up to 17.9 ± 1.11 dB and 10.7 ± 2.85 dB were attained by chirp-coded ultraharmonic imaging at 12 MHz and 30 MHz transmit frequencies, respectively. These results demonstrate the feasibility of performing ultraharmonic imaging at standard frame rates with clinically available IVUS catheters using chirp-coded excitation.

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Section: Introductionmentioning

confidence: 99%

Chirp-Coded Ultraharmonic Imaging with a Modified Clinical Intravascular Ultrasound System

et al. 2016

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“…While mere application of anti-ICAM-1 ELIP and subsequent intravascular ultrasound caused little signal change in the suspected areas of atheroma, application of NO-ELIP in combination with ultrasound activation demonstrated visually increased echogenicity, most probably due to increased vasodilation and permeability for targeted liposomes. Immunofluorescence microscopy and immunohistochemical staining of ICAM-1 confirmed pathological intimal thickening with high ICAM-1-expression as well as anti-ICAM-1 ELIP accumulation throughout all layers of the developing plaque after NO-pretreatment and ultrasound activation [49]. Although less closer to clinical translation than the preceding approach, the work of Kee and colleagues provides an additional path for the invasive molecular imaging of early atherosclerosis.…”

Section: Invasive Nanoparticle-enhanced Molecular Imaging Of Atheroscmentioning

confidence: 97%

Small Dimension—Big Impact! Nanoparticle-Enhanced Non-Invasive and Intravascular Molecular Imaging of Atherosclerosis In Vivo

et al. 2020

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Extensive translational research has provided considerable progress regarding the understanding of atherosclerosis pathophysiology over the last decades. In contrast, implementation of molecular in vivo imaging remains highly limited. In that context, nanoparticles represent a useful tool. Their variable shape and composition assure biocompatibility and stability within the environment of intended use, while the possibility of conjugating different ligands as well as contrast dyes enable targeting of moieties of interest on a molecular level and visualization throughout various imaging modalities. These characteristics have been exploited by a number of preclinical research approaches aimed at advancing understanding of vascular atherosclerotic disease, in order to improve identification of high-risk lesions prior to oftentimes fatal thromboembolic events. Furthermore, the combination of these targeted nanoparticles with therapeutic agents offers the potential of site-targeted drug delivery with minimized systemic secondary effects. This review gives an overview of different groups of targeted nanoparticles, designed for in vivo molecular imaging of atherosclerosis as well as an outlook on potential combined diagnostic and therapeutic applications.

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“…endothelium but also within plaques, lead to increased signal. This effect is presumably due to NO-induced increases in vascular permeability and, thus, accumulation of ICAM-1-targeted ELIPs within plaques (Kee et al 2014).…”

Section: Platelet Gpibα Mb Risk Areamentioning

confidence: 99%

Seeing the Invisible—Ultrasound Molecular Imaging

Kosareva

Abou‐Elkacem

Chowdhury

et al. 2020

Ultrasound in Medicine & Biology

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Ultrasound molecular imaging has been developed in the past two decades with the goal of non-invasively imaging disease phenotypes on a cellular level not depicted on anatomic imaging. Such techniques already play a role in pre-clinical research for the assessment of disease mechanisms and drug effects, and are thought to in the future contribute to earlier diagnosis of disease, assessment of therapeutic effects and patient-tailored therapy in the clinical field. In this review, we first describe the chemical composition and structure as well as the in vivo behavior of the ultrasound contrast agents that have been developed for molecular imaging. We then discuss the strategies that are used for targeting of contrast agents to specific cellular targets and protocols used for imaging. Next we describe pre-clinical data on imaging of thrombosis, atherosclerosis and microvascular inflammation and in oncology, including the pathophysiological principles underlying the selection of targets in each area. Where applicable, we also discuss efforts that are currently underway for translation of this technique into the clinical arena.

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Nitric Oxide Pretreatment Enhances Atheroma Component Highlighting in Vivo with Intercellular Adhesion Molecule-1-Targeted Echogenic Liposomes

Cited by 15 publications

References 52 publications

Chirp-Coded Ultraharmonic Imaging with a Modified Clinical Intravascular Ultrasound System

Chirp-Coded Ultraharmonic Imaging with a Modified Clinical Intravascular Ultrasound System

Small Dimension—Big Impact! Nanoparticle-Enhanced Non-Invasive and Intravascular Molecular Imaging of Atherosclerosis In Vivo

Seeing the Invisible—Ultrasound Molecular Imaging

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