Nitric Oxide: Probably the <i>in Vivo</i> Mediator of the Bisulfite’s Effects

Abstract

The use of bisulfite in food and beverage preservation, as well as in commercial goods and pharmaceuticals as antimicrobial agents is well known, but not very much is reported on its action in vivo. It has been stressed that its action is connected to the presence of NO, and the only reported/ hypothesized evidence concerns the possible interaction with GSNO (S-nitrosoglutathione), an NO releaser. In this light, we investigated the interaction between GSNO and the bisulfite in an aqueous medium at pH = 6.4; actually, a positive effect of the sulfite was evidenced. i.e., the S-nitrosoglutathione becomes a more efficient NO-releaser. But, the nitrite is the real pool of NO in vivo, therefore we investigate its interaction with the bisulfite in an aqueous acidic solution at pH = 6.4; this time, a definitely efficient and abundant NO release, 3.61 times higher compared to the GSNO, has been evidenced. Therefore, these results allow hypothesizing a fundamental role of NO in the bisulfite's action in vivo, or most probably the bisulfite acts simply as cofactor of NO-releasers.

The crucial role of non-enzymatic NO-production in plants. An EPR study

“…A similar mechanism could explain NO production also in plants (Yamasaki and Cohen, 2006). Thus, by reacting a NaNO2 acidic buffer solution (pH = 6.4) with HCAAs/PA/CAs, the released NO can be trapped through Fe ++ (DETC)2, and the paramagnetic NO adduct, NO-Fe ++ (DETC)2 thus formed, easily detected and identified by EPR spectroscopy (Grossi, 2014). This procedure had been previously used to detect NO in plants but, in those experiments, the spin-trap was directly introduced into the reaction pot (Caro andPuntarulo, 1999: Jasid, et al, 2006;Corpas, et al, 2004), and this changing the focal point of the process due to the reducing action of the trap (iron Fe ++ ), which will contribute to the release of NO.…”

“…The Fe ++ (DETC)2, iron (II) N,N-diethyldithiocarbamate, the NO-trap, was synthesized as previously reported (Grossi, 2009;2014). As shown in Scheme, by reacting an NaNO2 acidic buffer solution with HCAAs/PA/CAs, the released NO can be trapped through Fe ++ (DETC)2, and the paramagnetic NOadduct, NO-Fe ++ (DETC)2, easily detected and identified by EPR spectroscopy.…”

Nitric Oxide Reacts Very Fast with Hydrogen Sulfide, Alcohols, and Thiols to Produce HNO: Revised Rate Constants

“…We propose a two-step mechanism for reaction of the RS – species with two • NO molecules, − sequentially, as follows: …”

Role of Gasotransmitters in Oxidative Stresses, Neuroinflammation, and Neuronal Repair

“…In AD and other neurodegenerative dementias, NO has a role in neurodegeneration and neuronal cell death by creating NO-mediated neurotoxicity [174]. β -Amyloid triggers microglial and astrocytic NO generation in AD [138, 175, 176]. Firstly, A β stimulates CD 4+ T-cells and then activates microglial cells by producing cytokines [177].…”