Nitric oxide production is not required for dihydrosphingosine-induced cell death in tobacco BY-2 cells

Silva, Daniel Da; Lachaud, Christophe; Cotelle, Valérie; Brière, Christian; Grat, Sabine; Mazars, Christian; Thuleau, Patrice

doi:10.4161/psb.6.5.15126

Cited by 15 publications

(7 citation statements)

References 30 publications

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“…Accumulation of GapC in the nucleus was also observed in tobacco BY-2 cells treated with DHS (Testard et al, 2016). DHS is known to trigger an apoptotic-like response in tobacco, and in parallel, to increase ROS and RNS production (Lachaud et al, 2010, 2011; Da Silva et al, 2011). GapC1 accumulation in the nucleus was found to be dependent on NO levels in tobacco cells.…”

Section: Effects Of Redox Modifications Of Glycolytic Fermentative Amentioning

confidence: 99%

Consequences of Oxidative Stress on Plant Glycolytic and Respiratory Metabolism

2019

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Reactive oxygen species (ROS) and reactive nitrogen species (RNS) are present at low and controlled levels under normal conditions. These reactive molecules can increase to high levels under various biotic and abiotic conditions, resulting in perturbation of the cellular redox state that can ultimately lead to oxidative or nitrosative stress. In this review, we analyze the various effects that result from alterations of redox homeostasis on plant glycolytic pathway and tricarboxylic acid (TCA) cycle. Most documented modifications caused by ROS or RNS are due to the presence of redox-sensitive cysteine thiol groups in proteins. Redox modifications include Cys oxidation, disulfide bond formation, S -glutathionylation, S -nitrosylation, and S -sulfhydration. A growing number of proteomic surveys and biochemical studies document the occurrence of ROS- or RNS-mediated modification in enzymes of glycolysis and the TCA cycle. In a few cases, these modifications have been shown to affect enzyme activity, suggesting an operational regulatory mechanism in vivo . Further changes induced by oxidative stress conditions include the proposed redox-dependent modifications in the subcellular distribution of a putative redox sensor, NAD-glyceraldehyde-3P dehydrogenase and the micro-compartmentation of cytosolic glycolytic enzymes. Data from the literature indicate that oxidative stress may induce complex changes in metabolite pools in central carbon metabolism. This information is discussed in the context of our understanding of plant metabolic response to oxidative stress.

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Section: Effects Of Redox Modifications Of Glycolytic Fermentative Amentioning

confidence: 99%

Consequences of Oxidative Stress on Plant Glycolytic and Respiratory Metabolism

2019

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“…In tobacco BY-2 cells, sphinganine or dihydrosphingosine (d18:0, DHS) induce a calcium dependent PCD and trigger H 2 O 2 production via the activation of NADPH oxidase(s). They also promote NO production, which is required for cell death induction (Da Silva et al, 2011). NO accumulated in Cd-induced PCD and promoted Cd-induced Arabidopsis PCD by promoting MPK6-mediated caspase-3-like activation (Ye et al, 2013).…”

Section: No and Ros In Other Types Of Plant Cell Deathmentioning

confidence: 99%

Cross-talk of nitric oxide and reactive oxygen species in plant programed cell death

Wang¹,

Loake²,

Chu³

2013

Front. Plant Sci.

172

114

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In plants, programed cell death (PCD) is an important mechanism to regulate multiple aspects of growth and development, as well as to remove damaged or infected cells during responses to environmental stresses and pathogen attacks. Under biotic and abiotic stresses, plant cells exhibit a rapid synthesis of nitric oxide (NO) and a parallel accumulation of reactive oxygen species (ROS). Frequently, these responses trigger a PCD process leading to an intrinsic execution of plant cells. The accumulating evidence suggests that both NO and ROS play key roles in PCD. These redox active small molecules can trigger cell death either independently or synergistically. Here we summarize the recent progress on the cross-talk of NO and ROS signals in the hypersensitive response, leaf senescence, and other kinds of plant PCD caused by diverse cues.

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“…However, some studies have shown that NO is not related to the occurrence of PCD. Da Silva found that NO production in suspension cells of Nicotiana tobacillus was not necessary for PCD induced by hydrospingosine (Da Silva et al, 2011). It can be seen that NO has multiple effects on PCD in plants, which may mainly depend on various factors, such as cell types, cellular redox status, local NO ux and dose and the like (Wang et al, 2010).…”

Section: Discussionmentioning

confidence: 99%

Effects of Programmed Cell Death Induced by NO on Pollen Viability After Cryopreservation

Ren¹,

Zhou²,

Zhang³

et al. 2021

Preprint

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Low activity level of biomaterials after cryopreservation is a bottleneck problem that limited the application of this technology. At present, the mechanism of viability decline after cryopreservation is not fully understood. In this study, the effects of nitric oxide (NO) on programmed cell death (PCD) and its relationship with viability were investigated, using Paeonia lactiflora 'Fen Yu Nu' pollen with significantly decreased viability after cryopreservation. The results showed that: the activity of caspase-3-like and caspase-9-like protease and the apoptosis rate of pollen cells were significantly increased, the expression level of promoting PCD (pro-PCD) gene was up-regulated, while the expression level of inhibiting PCD (anti-PCD) gene was down-regulated after preserved in liquid nitrogen (LN); the NO content in pollen cells increased significantly after LN preserved. The results of correlation analysis showed that NO was significantly correlated with pollen viability and all indicators of PCD. The addition of NO carrier SNP after LN storage reduced pollen viability, increased endogenous NO content, decreased mitochondrial membrane potential level, activated caspase-3-like and caspase-9-like protease in pollen cells, and increased cell apoptosis rate, and the expression levels of pro-PCD genes PDCD2 and ATG8CL were significantly up-regulated, while the expression levels of anti-PCD genes DAD1, BI-1 and LSD1 were significantly down-regulated; and the addition of NO scavenger c-PTIO produced the opposite effect of SNP. It was suggested that NO induced the PCD during the cryopreservation of pollen, which was one of the reasons for the significant decrease of pollen viability after cryopreservation.

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Nitric oxide production is not required for dihydrosphingosine-induced cell death in tobacco BY-2 cells

Cited by 15 publications

References 30 publications

Consequences of Oxidative Stress on Plant Glycolytic and Respiratory Metabolism

Consequences of Oxidative Stress on Plant Glycolytic and Respiratory Metabolism

Cross-talk of nitric oxide and reactive oxygen species in plant programed cell death

Effects of Programmed Cell Death Induced by NO on Pollen Viability After Cryopreservation

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