Nitric oxide reductases in bacteria

Hendriks, Janneke; Oubrie, Arthur; Castresana, José; Urbani, Andrea; Gemeinhardt, Sabine; Saraste, Matti

doi:10.1016/s0005-2728(00)00161-4

Cited by 187 publications

(197 citation statements)

References 43 publications

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“…2. Nitric oxide reductase is the primary enzyme in a chain of catalytic reactions leading to the production of N 2 O (Hino et al, 2010;Hendriks et al, 2000). The catalytic cycle involving production of N 2 O from NO has yet to be completely understood with respect to the formation of the N-N double bond, the complexity of the structural information of nitric oxide reductase, the proton transfer pathway into nitric oxide reductase (Tosha and Shiro, 2013), and the very short lifetime of the intermediate states of the molecules (Collman et al, 2008).…”

Section: Discussionmentioning

confidence: 99%

Continuous measurements of nitrous oxide isotopomers during incubation experiments

et al. 2018

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Abstract. Nitrous oxide (N 2 O) is an important and strong greenhouse gas in the atmosphere. It is produced by microbes during nitrification and denitrification in terrestrial and aquatic ecosystems. The main sinks for N 2 O are turnover by denitrification and photolysis and photo-oxidation in the stratosphere. In the linear N=N=O molecule 15 N substitution is possible in two distinct positions: central and terminal. The respective molecules, 14 N 15 N 16 O and 15 N 14 N 16 O, are called isotopomers. It has been demonstrated that N 2 O produced by nitrifying or denitrifying microbes exhibits a different relative abundance of the isotopomers. Therefore, measurements of the site preference (difference in the abundance of the two isotopomers) in N 2 O can be used to determine the source of N 2 O, i.e., nitrification or denitrification. Recent instrument development allows for continuous positiondependent δ 15 N measurements at N 2 O concentrations relevant for studies of atmospheric chemistry. We present results from continuous incubation experiments with denitrifying bacteria, Pseudomonas fluorescens (producing and reducing N 2 O) and Pseudomonas chlororaphis (only producing N 2 O). The continuous measurements of N 2 O isotopomers reveals the transient isotope exchange among KNO 3 , N 2 O, and N 2 . We find bulk isotopic fractionation of −5.01 ‰ ± 1.20 for P. chlororaphis, in line with previous results for production from denitrification. For P. fluorescens, the bulk isotopic fractionation during production of N 2 O is −52.21 ‰ ± 9.28 and 8.77 ‰ ± 4.49 during N 2 O reduction.The site preference (SP) isotopic fractionation for P. chlororaphis is −3.42 ‰ ± 1.69. For P. fluorescens, the calculations result in SP isotopic fractionation values of 5.73 ‰ ± 5.26 during production of N 2 O and 2.41 ‰ ± 3.04 during reduction of N 2 O. In summary, we implemented continuous measurements of N 2 O isotopomers during incubation of denitrifying bacteria and believe that similar experiments will lead to a better understanding of denitrifying bacteria and N 2 O turnover in soils and sediments and ultimately hands-on knowledge on the biotic mechanisms behind greenhouse gas exchange of the globe.

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Section: Discussionmentioning

confidence: 99%

Continuous measurements of nitrous oxide isotopomers during incubation experiments

et al. 2018

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“…Toyoda et al (2002) proposed that nitrification should yield δ 15 N α enriched N 2 O due to the existence of a hyponitrite (-ONNO-) intermediary followed by the selective breakage of the NO bond where the lightest isotopes are located, based on the ZPE (zero point energy) for N 2 O (Zuñiga et al, 2003). This mechanism has been described for some nitric oxide reductases (Hendriks et al, 2000;Wasser et al, 2002). Culture experiments carried out by Sutka et al (2006) show that ammonium oxidation carried out by ammonium oxidizers (i.e.…”

Section: General Water Column Characteristicsmentioning

confidence: 99%

Nitrous oxide distribution and its origin in the central and eastern South Pacific Subtropical Gyre

Charpentier¹,

Farı́as²,

Yoshida

et al. 2007

Biogeosciences

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Abstract. The mechanisms of microbial nitrous oxide (N 2 O) production in the ocean have been the subject of many discussions in recent years. New isotopomeric tools can further refine our knowledge of N 2 O sources in natural environments. This study compares hydrographic, N 2 O concentration, and N 2 O isotopic and isotopomeric data from three stations along a coast-perpendicular transect in the South Pacific Ocean, extending from the center (Sts. GYR and EGY) of the subtropical oligotrophic gyre (∼26 • S; 114 • W) to the upwelling zone (St. UPX) off the central Chilean coast (∼34 • S). Although AOU/N 2 O and NO − 3 trends support the idea that most of the N 2 O (mainly from intermediate water (200-600 m)) comes from nitrification, N 2 O isotopomeric composition (intramolecular distribution of 15 N isotopes) expressed as SP (site preference of 15 N) shows low values (10 to 12‰) that could be attributed to the production through of microbial nitrifier denitrification (reduction of nitrite to N 2 O mediated by ammonium oxidizers). The coincidence of this SP signal with high -stability layer, where sinking organic particles can accumulate, suggests that N 2 O could be produced by nitrifier denitrification inside particles. It is postulated that deceleration of particles in the pycnocline can modify the advection -diffusion balance inside particles, allowing the accumulation of nitrite and O 2 depletion suitable for nitrifier denitrication. As lateral advection seems to be relatively insignificant in the gyre, in situ nitrifier denitrification could account for 40-50% of the N 2 O produced in this Correspondence to: J. Charpentier (jcharpentier@profc.udec.cl) layer. In contrast, coastal upwelling system is characterized by O 2 deficient condition and some N deficit in a eutrophic system. Here, N 2 O accumulates up to 480% saturation, and isotopic and isotopomer signals show highly complex N 2 O production processes, which presumably reflect both the effect of nitrification and denitrification at low O 2 levels on N 2 O production, but net N 2 O consumption by denitrification was not observed.

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“…The pathways of biological N 2 O production include partial dissimilatory nitrate or nitrite reduction (denitrification), nitrifier denitrification (Wrage et al, 2001), ammonia (hydroxylamine) oxidation and NOx detoxification (also known as the 'nitrosative stress' pathway) (Hendriks et al, 2000). Nitrification-related pathways (nitrifier denitrification and ammonia oxidation) mediated by ammonia-oxidizing bacteria (AOB) are known to be a major source of N 2 O production from terrestrial environments (Gö dde and Conrad, 1999).…”

Section: Introductionmentioning

confidence: 99%

Isotopic signatures of N2O produced by ammonia-oxidizing archaea from soils

Well²,

et al. 2013

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N 2 O gas is involved in global warming and ozone depletion. The major sources of N 2 O are soil microbial processes. Anthropogenic inputs into the nitrogen cycle have exacerbated these microbial processes, including nitrification. Ammonia-oxidizing archaea (AOA) are major members of the pool of soil ammonia-oxidizing microorganisms. This study investigated the isotopic signatures of N 2 O produced by soil AOA and associated N 2 O production processes. All five AOA strains (I.1a, I.1a-associated and I.1b clades of Thaumarchaeota) from soil produced N 2 O and their yields were comparable to those of ammonia-oxidizing bacteria (AOB). The levels of site preference (SP), d 15 N bulk and d 18 O -N 2 O of soil AOA strains were 13-30%, À 13 to À 35% and 22-36%, respectively, and strains MY1-3 and other soil AOA strains had distinct isotopic signatures. A 15 N-NH 4 þ -labeling experiment indicated that N 2 O originated from two different production pathways (that is, ammonia oxidation and nitrifier denitrification), which suggests that the isotopic signatures of N 2 O from AOA may be attributable to the relative contributions of these two processes. The highest N 2 O production yield and lowest site preference of acidophilic strain CS may be related to enhanced nitrifier denitrification for detoxifying nitrite. Previously, it was not possible to detect N 2 O from soil AOA because of similarities between its isotopic signatures and those from AOB. Given the predominance of AOA over AOB in most soils, a significant proportion of the total N 2 O emissions from soil nitrification may be attributable to AOA.

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Nitric oxide reductases in bacteria

Cited by 187 publications

References 43 publications

Continuous measurements of nitrous oxide isotopomers during incubation experiments

Continuous measurements of nitrous oxide isotopomers during incubation experiments

Nitrous oxide distribution and its origin in the central and eastern South Pacific Subtropical Gyre

Isotopic signatures of N2O produced by ammonia-oxidizing archaea from soils

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