Nitric oxide-releasing vascular grafts: A therapeutic strategy to promote angiogenic activity and endothelium regeneration

Kabirian, Fatemeh; Milan, Peiman Brouki; Zamanian, Ali; Heying, Ruth; Mozafari, Masoud

doi:10.1016/j.actbio.2019.05.002

Cited by 60 publications

(54 citation statements)

References 66 publications

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“…It can be concluded that the prepared SDVGs are noncytotoxic both in short‐term (24 hr) and longer‐term (72 hr) periods. The findings are in agreement with previous reports of zero toxicity and higher proliferation rate of ECs after NO treatment (Goudie, Pant, & Handa, ; Kabirian, Milan, et al, ; Yang et al, ).…”

Section: Resultssupporting

confidence: 93%

“…The SDVGs were successfully prepared from PLA filament using a FDM 3D printer according to a predesigned CAD model. Previously, we fabricated biodegradable 3D‐printed SDVGs from PLA using the FDM machine (Kabirian et al, ; Kabirian, Ditkowski, et al, ; Kabirian, Milan, et al, ). In this study, to improve the healing and antibacterial properties of such SDVGs, a novel NO‐releasing system was successfully incorporated.…”

Section: Resultsmentioning

confidence: 99%

“…3D printing is an advantageous procedure to design and fabricate highly accurate and reproducible custom‐based structures which can promisingly be used for cardiovascular tissue engineering applications (Kabirian, Ditkowski, et al, ; Kabirian, Milan, et al, ). In the current study, SDVGs were successfully fabricated using the FDM 3D printing technology with PLA filaments (Figure a).…”

Section: Resultsmentioning

confidence: 99%

“…HUVECs at a concentration of 10 4 cells/well were seeded in 96‐well plates ( n = 3) and incubated at 37°C. After obtaining a confluent monolayer, a vertical scratch was created with a 100‐μl pipette tip across the center of the well, the culture media was aspirated and cells were rinsed twice with PBS to remove detached cells (Kabirian, Milan, Zamanian, Heying, & Mozafari, ). The cell‐free scratch wound is a model of graft borders after implantation where EC migration from the adjacent native vessel is crucial for endothelialization and graft integration.…”

Section: Methodsmentioning

confidence: 99%

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Additively manufactured small‐diameter vascular grafts with improved tissue healing using a novel SNAP impregnation method

et al. 2019

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The vascular network has a complex architecture such as branches, curvatures, and bifurcations which is even more complicated in view of individual patients' defect anatomy requiring custom‐specifically designed vascular implants. In this work, 3D printing is used to overcome these challenges and a new shorter impregnation method was developed to incorporate S‐nitroso‐N‐acetyl‐d‐penicillamine (SNAP) as a nitric oxide (NO) donor to printed grafts. The 3D‐printed small‐diameter vascular grafts (SDVGs) were impregnated with SNAP solution during SNAP synthesis (S1) or with SNAP dissolved in methanol (S2). The advantage of the newly developed S1 impregnation method is the elimination of the synthesis step by direct impregnation inside the S1 solution. Scanning electron microscopy imaging reveals the successful crystal formation in both methods. The results demonstrate that both S1‐ and S2‐impregnated grafts, after covering with polycaprolactone topcoat, can release NO in a controlled manner and in the physiological range (0.5–4.0 × 10−10 mol cm−2 min−1) over a 15 days period. The created grafts with a NO‐releasing surface have also shown bactericidal effect while the healing properties of the implant were improved by promoting migration and proliferation of endothelial cells (ECs). These results suggest that incorporation of 3D printing technology with the newly developed S1 impregnation of SNAP can optimize and shorten the manufacturing process of the next generation of patient‐based antibacterial SDVGs with a higher attraction for ECs.

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Section: Resultssupporting

confidence: 93%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Additively manufactured small‐diameter vascular grafts with improved tissue healing using a novel SNAP impregnation method

et al. 2019

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“…3D-printed PCL-based vascular grafts incorporated with SNAP were developed to combine the advantages of 3D printing technology and NO-releasing system (Figure 5). [203] These NO-releasing grafts could not only maintain sustained NO release up to two weeks in the physiological environment, but also enhance bactericidal property and endothelialization ability for both in vitro and in vivo.…”

Section: Growth Factorsmentioning

confidence: 96%

Surface Engineering of Cardiovascular Devices for Improved Hemocompatibility and Rapid Endothelialization

Zhao

Feng

2020

Adv Healthcare Materials

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Cardiovascular devices have been widely applied in the clinical treatment of cardiovascular diseases. However, poor hemocompatibility and slow endothelialization on their surface still exist. Numerous surface engineering strategies have mainly sought to modify the device surface through physical, chemical, and biological approaches to improve surface hemocompatibility and endothelialization. The alteration of physical characteristics and pattern topographies brings some hopeful outcomes and plays a notable role in this respect. The chemical and biological approaches can provide potential signs of success in the endothelialization of vascular device surfaces. They usually involve therapeutic drugs, specific peptides, adhesive proteins, antibodies, growth factors and nitric oxide (NO) donors. The gene engineering can enhance the proliferation, growth, and migration of vascular cells, thus boosting the endothelialization. In this review, the surface engineering strategies are highlighted and summarized to improve hemocompatibility and rapid endothelialization on the cardiovascular devices. The potential outlook is also briefly discussed to help guide endothelialization strategies and inspire further innovations. It is hoped that this review can assist with the surface engineering of cardiovascular devices and promote future advancements in this emerging research field.

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Self‐Propelling Nanomotors Integrated with Biofilm Microenvironment‐Activated NO Release to Accelerate Healing of Bacteria‐Infected Diabetic Wounds

Xie

Huang

Peng

et al. 2022

Adv Healthcare Materials

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Diabetic foot ulcer (DFU) treatment is challenged by persistent bacterial infection and hyperglycemia-caused vascular dysplasia. Herein, self-propelled nanomotors are designed to achieve biofilm microenvironment (BME)-activated multistage release of NO for effective sterilization and subsequent angiogenesis promotion. CaO 2 nanoparticles (NPs) are capped with PDA layers, followed by complexation with Fe 2+ and surface grafting of cysteine-NO to obtain Janus Ca@PDA Fe -CNO NPs. In response to low pH in BME, the decomposition of CaO 2 cores generates O 2 from one side of Janus NPs to propel biofilm penetration, and the released H 2 O 2 and Fe 2+ produce •OH through Fenton reaction. The concurrent glutathione-triggered release of NO can be converted into reactive nitrogen species, which exhibit significantly higher bactericidal efficacy than those with only generation of •OH or NO. The slow release of NO for an extended time period promotes endothelial cell proliferation and migration. On Staphylococcus aureus-infected skin wounds of diabetic mice, NP treatment eliminates bacterial infections and significantly elevates blood vessel densities, leading to full wound recovery and regeneration of arranged collagen fibers and skin accessories. Thus, the self-propelling and multistage release of NO provide a feasible strategy to combat biofilm infection without using any antibiotics and accelerate angiogenesis and wound healing for DFU treatment.

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Nitric oxide-releasing vascular grafts: A therapeutic strategy to promote angiogenic activity and endothelium regeneration

Cited by 60 publications

References 66 publications

Additively manufactured small‐diameter vascular grafts with improved tissue healing using a novel SNAP impregnation method

Additively manufactured small‐diameter vascular grafts with improved tissue healing using a novel SNAP impregnation method

Surface Engineering of Cardiovascular Devices for Improved Hemocompatibility and Rapid Endothelialization

Self‐Propelling Nanomotors Integrated with Biofilm Microenvironment‐Activated NO Release to Accelerate Healing of Bacteria‐Infected Diabetic Wounds

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