Nitric oxide-sensitive guanylyl cyclase: structure and regulation

Koesling, Doris; Russwurm, Michael; Mergia, Evanthia; Müllershausen, Florian; Friebe, Andreas

doi:10.1016/j.neuint.2004.03.011

Cited by 137 publications

(116 citation statements)

References 72 publications

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“…Once released, NO diffuses through the cell membrane and enters the cytosol, where it meets the target protein, such as sGC (19,20). NO may bind to the protoporphyrin ring of sGC, causing activation of the enzyme and leading to the formation of cGMP (21,22). As an intracellular signaling molecule, cGMP plays an important role in the regulation of various cellular events and may exert its effects through PKGs, which catalyze the phosphorylation at serine/threonine residues on substrate proteins, thereby altering their activities (23).…”

Section: Discussionmentioning

confidence: 99%

Nitric oxide/cyclic guanosine monophosphate inducers sodium nitroprusside and L-arginine inhibit the proliferation of gastric cancer cells via the activation of type II cyclic guanosine monophosphate-dependent protein kinase

Yao

Zhu

et al. 2015

Oncology Letters

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Abstract. Nitric oxide (NO) may activate soluble guanylyl cyclase (sGC), resulting in the increase of intracellular cyclic guanosine monophosphate (cGMP), a key molecule in the activation of type II cGMP-dependent protein kinase (PKG II). In our previous study, the membrane-permeable cGMP analogue 8-pCPT-cGMP was used to activate PKG II. The aim of the present study was to investigate whether NO/sGC-induced endogenous cGMP is able to activate PKG II and induce the corresponding effects. In the AGS gastric cancer cell line, the expression of PKG II was increased by infecting the cells with an adenoviral construct encoding PKG II cDNA (Ad-PKG II) and the activation of PKG II was induced by 8-pCPT-cGMP (positive control), the NO donor sodium nitroprusside (SNP) and the NO precursor L-arginine. ELISA was performed to detect the concentration of cGMP in AGS cells and the Cell Counting Kit-8 was used to analyze the proliferation of differently treated cells. Western blot analysis was used to detect the expression and phosphorylation of associated proteins. The results demonstrated that the level of cGMP was increased in cells treated with the NO donor or precursor. There was an obvious increase of Ser239 phosphorylation of the vasodilator-stimulated phosphoprotein, representing the increase in the activity of PKG II. The epidermal growth factor (EGF)-induced proliferation of AGS cells was inhibited by infection with Ad-PKG II and treatment with SNP or L-arginine. In addition, EGF-induced tyrosine phosphorylation of the EGF receptor (EGFR) and tyrosine/serine phosphorylation of extracellular signal-regulated kinase (ERK) were also inhibited by infection with Ad-PKG II and treatment with the NO donor or precursor. These data indicated that NO donors and precursors may activate the expression of PKG II, thereby blocking EGF-initiated signaling of the mitogen-activated protein kinase/ERK pathway and inhibiting EGF-induced proliferative activity through preventing the phosphorylation of EGFR at Tyr1068.

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Section: Discussionmentioning

confidence: 99%

Nitric oxide/cyclic guanosine monophosphate inducers sodium nitroprusside and L-arginine inhibit the proliferation of gastric cancer cells via the activation of type II cyclic guanosine monophosphate-dependent protein kinase

Yao

Zhu

et al. 2015

Oncology Letters

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“…It is composed of a cytosolic heterodimer of sGCa and sGCb subunits. 28 Among these subunits, sGCb1 acts as the common dimerizing subunit for the two sGCa subunits and is crucial for the expression of sGC in cells. Knockdown of the sGCb1 subunit gene results in the lack of sGC expression.…”

Section: Discussionmentioning

confidence: 99%

Ultrastructural features and possible functional role of kit-positive interstitial cells in the guinea pig corpus cavernosum

Song

et al. 2011

Int J Impot Res

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The objective of the present study was to identify kit-positive interstitial cells (ICs) in guinea pig corpus cavernosum and examine their relationships with adjacent smooth muscle cells (SMCs) and intramural nerves. In addition, we investigated the possible involvement of ICs in nitric oxide (NO)-mediated relaxation of corpus cavernosum smooth muscle (CCSM). ICs were identified by their immunoreactivity to the kit receptor, a cell surface marker encoded by c-kit proto-oncogene and specific for interstitial cells of Cajal. ICs were abundantly distributed in guinea pig corporal tissues. Ultrastructural investigation by conventional transmission electron microscopy revealed the ultrastructural features of ICs and gap junctions located between ICs and adjacent SMCs, furthermore, a close contact between ICs and intramural nerves for the first time. Western blot analysis of purified ICs by fluorescence-activated cell sorting revealed coexpression of soluble guanylate cyclase (sGC)a1, sGCb1 and kit receptor tyrosine kinase protein in them. These observations imply that ICs express the NO-sensitive sGC molecule and may be involved in the NO-mediated relaxation of CCSM in the guinea pig corpus cavernosum.

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“…NO-GC was initially thought to be entirely cytosolic and was thus named "soluble GC", however it has also been found associated to membranes (Zabel et al 2002). NO-GC is composed of an α and a β subunit and both subunits are required for catalytic activity (Koesling et al 2004). Each subunit has a regulatory domain which contains a prosthetic heme group that is the NO-binding site, a catalytic domain that shares sequence homology with the corresponding domains in pGC and adenylyl cyclases and a central domain which allows subunit dimerization (Foster et al 1999) (Fig.…”

Section: Guanylyl Cyclisesmentioning

confidence: 99%

Mechanisms Involved in the Remyelinating Effect of Sildenafil

Díaz-Lucena

Gutièrrez‐Mecinas

Moreno

et al. 2017

J Neuroimmune Pharmacol

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ADVERTIMENT. Lʼaccés als continguts dʼaquesta tesi doctoral i la seva utilització ha de respectar els drets de la persona autora. Pot ser utilitzada per a consulta o estudi personal, així com en activitats o materials dʼinvestigació i docència en els termes establerts a lʼart. 32 del Text Refós de la Llei de Propietat Intel·lectual (RDL 1/1996). Per altres utilitzacions es requereix lʼautorització prèvia i expressa de la persona autora. En qualsevol cas, en la utilització dels seus continguts caldrà indicar de forma clara el nom i cognoms de la persona autora i el títol de la tesi doctoral. No sʼautoritza la seva reproducció o altres formes dʼexplotació efectuades amb finalitats de lucre ni la seva comunicació pública des dʼun lloc aliè al servei TDX. Tampoc sʼautoritza la presentació del seu contingut en una finestra o marc aliè a TDX (framing). Aquesta reserva de drets afecta tant als continguts de la tesi com als seus resums i índexs.ADVERTENCIA. El acceso a los contenidos de esta tesis doctoral y su utilización debe respetar los derechos de la persona autora. Puede ser utilizada para consulta o estudio personal, así como en actividades o materiales de investigación y docencia en los términos establecidos en el art. 32 del Texto Refundido de la Ley de Propiedad Intelectual (RDL 1/1996). Para otros usos se requiere la autorización previa y expresa de la persona autora. En cualquier caso, en la utilización de sus contenidos se deberá indicar de forma clara el nombre y apellidos de la persona autora y el título de la tesis doctoral. No se autoriza su reproducción u otras formas de explotación efectuadas con fines lucrativos ni su comunicación pública desde un sitio ajeno al servicio TDR. Tampoco se autoriza la presentación de su contenido en una ventana o marco ajeno a TDR (framing). Esta reserva de derechos afecta tanto al contenido de la tesis como a sus resúmenes e índices.WARNING.

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Nitric oxide-sensitive guanylyl cyclase: structure and regulation

Cited by 137 publications

References 72 publications

Nitric oxide/cyclic guanosine monophosphate inducers sodium nitroprusside and L-arginine inhibit the proliferation of gastric cancer cells via the activation of type II cyclic guanosine monophosphate-dependent protein kinase

Nitric oxide/cyclic guanosine monophosphate inducers sodium nitroprusside and L-arginine inhibit the proliferation of gastric cancer cells via the activation of type II cyclic guanosine monophosphate-dependent protein kinase

Ultrastructural features and possible functional role of kit-positive interstitial cells in the guinea pig corpus cavernosum

Mechanisms Involved in the Remyelinating Effect of Sildenafil

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