Nitric Oxide Signaling Contributes to Ectopic Orofacial Neuropathic Pain

Sugiyama, Tomohisa; Shinoda, Masamichi; Watase, Tetsuro; Honda, Kuniya; Ito, Risa; Kaji, Kaori; Urata, Kentaro; Lee, J.; Ohara, Kinuyo; Takahashi, Osamu; Echizenya, Sumire; Iwata, Koichi

doi:10.1177/0022034513509280

Cited by 32 publications

(29 citation statements)

References 31 publications

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“…Thirty‐five rats were anesthetized with sodium pentobarbital (50 mg kg −1 intraperitoneally; Schering‐Plough, Whitehouse Station, NJ, USA) and placed on a warm mat (37°C). Briefly, a small incision was made on the surface of the left facial skin, and the masseter muscle and the surface of the alveolar bone covering the left IAN were removed to expose the IAN . The exposed IAN was lifted, transected, and returned to the mandibular canal without leaving a discernable gap between the stumps of the IAN ( n = 24).…”

Section: Methodsmentioning

confidence: 99%

Low‐intensity pulsed ultrasound accelerates nerve regeneration following inferior alveolar nerve transection in rats

Sato

Motoyoshi

Shinoda

et al. 2016

European J Oral Sciences

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Inferior alveolar nerve (IAN) injury, which is frequently caused by orofacial surgery or trauma, induces sensory loss in orofacial regions innervated by the IAN. However, no effective treatment for orofacial sensory loss currently exists. We determined whether sensory loss in facial skin above the mental foramen following IAN transection was recovered by exposure of the transected IAN to low-intensity pulsed ultrasound (LIPUS). Inferior alveolar nerve transection (IANX) was performed in 7-wk-old male Sprague-Dawley rats. On day 7 after IANX, the effect of daily LIPUS (from day 0) on the transected IAN, in terms of sensitivity to mechanical stimulation of the facial skin above the mental foramen, was examined. Moreover, the number of trigeminal ganglion (TG) neurons innervating the facial skin above the mental foramen of rats with IANX treated daily with LIPUS was counted using the retrograde neurotracing technique. Daily exposure of the transected IAN to LIPUS significantly promoted recovery of the head-withdrawal threshold in response to mechanical stimulation of the facial skin above the mental foramen, and the number of TG neurons innervating the facial skin above mental foramen was significantly increased in rats with IANX treated daily with LIPUS compared with sham or LIPUS-unexposed rats. Daily treatment of stumps of the transected IAN with LIPUS facilitated morphological and functional regeneration, suggesting that LIPUS is an effective and novel therapy for IAN injury.

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Section: Methodsmentioning

confidence: 99%

Low‐intensity pulsed ultrasound accelerates nerve regeneration following inferior alveolar nerve transection in rats

Sato

Motoyoshi

Shinoda

et al. 2016

European J Oral Sciences

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“…To our knowledge, all other craniofacial neuropathic pain models involve damage of sensory nerves (Eriksson et al, 2005; Sugiyama et al, 2013; Vos et al, 1994). The results obtained here demonstrate that injury to the facial nerve, which we show to be devoid of detected trigeminal somatosensory afferents from the skin, is also sufficient to create neuropathic pain.…”

Section: Discussionmentioning

confidence: 99%

“…To date, several models of craniofacial neuropathic pain have been developed, involving lesions of the sensory infraorbital (Eriksson et al, 2005; Vos et al, 1994), or sensory inferior alveolar nerves (Sugiyama et al, 2013). However, it is not yet known whether injury of cranial motor nerves is sufficient to induce neuropathic pain, similar to the spinal system.…”

Section: Introductionmentioning

confidence: 99%

Constriction of the buccal branch of the facial nerve produces unilateral craniofacial allodynia

Lewis

Grace

Hutchinson

et al. 2017

Brain, Behavior, and Immunity

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Despite pain being a sensory experience, studies of spinal cord ventral root damage have demonstrated that motor neuron injury can induce neuropathic pain. Whether injury of cranial motor nerves can also produce nociceptive hypersensitivity has not been addressed. Herein, we demonstrate that chronic constriction injury (CCI) of the buccal branch of the facial nerve results in long-lasting, unilateral allodynia in the rat. An anterograde and retrograde tracer (3000MW tetramethylrhodamine-conjugated dextran) was not transported to the trigeminal ganglion when applied to the injury site, but was transported to the facial nucleus, indicating that this nerve branch is not composed of trigeminal sensory neurons. Finally, intracisterna magna injection of interleukin-1 (IL-1) receptor antagonist reversed allodynia, implicating the pro-inflammatory cytokine IL-1 in the maintenance of neuropathic pain induced by facial nerve CCI. These data extend the prior evidence that selective injury to motor axons can enhance pain to supraspinal circuits by demonstrating that injury of a facial nerve with predominantly motor axons is sufficient for neuropathic pain, and that the resultant pain has a neuroimmune component.

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“…Neuronal NOS (nNOS, type 1 NOS) is one forms of NOS [7]. Over the past decades, by using inhibitors of NO biosynthesis, NO donors and animal nociceptive models, knowledge of the role of NO in types of pain has been discovered both in central and peripheral system [8][9][10][11].…”

Section: Introductionmentioning

confidence: 99%

Role of laser fluence in protein synthesis of cultured DRG neurons following low-level laser irradiation

et al. 2014

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Low-level lasers have been used to relieve pain in clinical for many years. But the mechanism is not fully clear. In animal models, nitric oxide (NO) has been reported involving in the transmission and modulation of nociceptive signals.So the objective of this study was to establish whether low-level laser with different fluence could stimulate the production of nitric oxide synthese (NOS), which produces NO in cultured primary dorsal root ganglion neurons (DRG neurons). The primary DRG neurons were isolated from healthy Sprague Dawley rats (8-12 weeks of age) and spread on 35 mm culture dishes specially used for confocal microscopy. 24 hours after spreading, cells were irradiated with 658 nm laser for two consecutive days at the energy density of 20, 40, 60 and 80 mJ· cm -2 respectively. Control groups were not exposed to the laser, but were kept under the same conditions as the irradiated ones. The synthesis of NOS after laser irradiation was detected by immunofluorescence assay, and the changes of NOS were evaluated using confocal microscopy and Image J software. The results showed that all the laser fluence could promote the production of NOS in DRG neurons, especially the 60 mJ· cm -2 . These results demonstrated that low-level laser irradiation could modify protein synthesis in a dose-or fluence-dependent manner, and indicated that low-level laser irradiation might achieve the analgesic effect through modulation of NO production.

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Nitric Oxide Signaling Contributes to Ectopic Orofacial Neuropathic Pain

Cited by 32 publications

References 31 publications

Low‐intensity pulsed ultrasound accelerates nerve regeneration following inferior alveolar nerve transection in rats

Low‐intensity pulsed ultrasound accelerates nerve regeneration following inferior alveolar nerve transection in rats

Constriction of the buccal branch of the facial nerve produces unilateral craniofacial allodynia

Role of laser fluence in protein synthesis of cultured DRG neurons following low-level laser irradiation

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