Nitric Oxide Signaling in Plant Responses to Abiotic Stresses

Qiao, Weihua; Fan, Liu‐Min

doi:10.1111/j.1744-7909.2008.00759.x

Cited by 173 publications

(113 citation statements)

References 107 publications

(151 reference statements)

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“…2 E and F; P < 0.05). The opposite effect of NO on the activities of SNO1 and PDX3 we observed could be a competitive result between these two salvage pathway enzymes facing NO treatment, and the increase of SNO1 activity in response to SNP treatment may suggest a prominent role of SNO1 enzyme in stress responses because NO is a well-known stress-induced signaling molecule (43,44). Consistent with this concept, previous study showed that the expression of SOS4 (or SNO1) is regulated by several environmental stimuli (36).…”

Section: Resultsmentioning

confidence: 87%

Nitric oxide negatively regulates AKT1-mediated potassium uptake through modulating vitamin B6 homeostasis in Arabidopsis

Xia

Kong

Xue

et al. 2014

Proc. Natl. Acad. Sci. U.S.A.

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Nitric oxide (NO), an active signaling molecule in plants, is involved in numerous physiological processes and adaptive responses to environmental stresses. Under high-salt conditions, plants accumulate NO quickly, and reorganize Na + and K + contents. However, the molecular connection between NO and ion homeostasis is largely unknown. Here, we report that NO lowers K + channel AKT1-mediated plant K + uptake by modulating vitamin B6 biosynthesis. In a screen for Arabidopsis NO-hypersensitive mutants, we isolated sno1 (sensitive to nitric oxide 1), which is allelic to the previously noted mutant sos4 (salt overly sensitive 4) that has impaired Na + and K + contents and overproduces pyridoxal 5′-phosphate (PLP), an active form of vitamin B6. We showed that NO increased PLP and decreased K + levels in plant. NO induced SNO1 gene expression and enzyme activity, indicating that NO-triggered PLP accumulation mainly occurs through SNO1-mediated vitamin B6 salvage biosynthetic pathway. Furthermore, we demonstrated that PLP significantly repressed the activity of K + channel AKT1 in the Xenopus oocyte system and Arabidopsis root protoplasts. Together, our results suggest that NO decreases K + absorption by promoting the synthesis of vitamin B6 PLP, which further represses the activity of K + channel AKT1 in Arabidopsis. These findings reveal a previously unidentified pivotal role of NO in modulating the homeostasis of vitamin B6 and potassium nutrition in plants, and shed light on the mechanism of NO in plant acclimation to environmental changes.genetic approach | electrophysiological studies | potassium nutrition N itric oxide (NO) acts as a crucial signaling molecule in various physiological processes in plants, such as seed germination and dormancy (1, 2), root development (3), leaf senescence (4, 5), floral transition (6), stomatal movement (7, 8), iron homeostasis (9, 10), and hormone responses (11,12). NO production is altered when plants are subjected to abiotic or biotic stresses (13,14). High salt, a major environmental factor that limits agriculture yield, induces a quick endogenous NO accumulation in plants (15,16), and triggers enhanced Na + influx and reduced K + absorption in the root (17). Both endogenously produced NO and exogenously applied NO have been proposed to enhance plant salt tolerance (18-21) by attenuating high saltinduced increases in the Na + to K + ratio. Genetic analysis showed that K + nutrition, but not Na + , plays critical role in plant salt tolerance (22). However, the molecular basis of NO effect on K + or Na + content is elusive.K + levels in plant tissues are determined by K + uptake and translocation, which are mediated by a large number of transporters and channels. In Arabidopsis, the K + channel AKT1 (23, 24) and K + transporter AtHAK5 (25) are the two major molecular entities responsible for K + absorption from the environment (26-28). AKT1 contributes to K + acquisition over a wide range of external K + concentrations (10 μM-10 mM), whereas AtHAK5 mediates limited uptake capac...

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Section: Resultsmentioning

confidence: 87%

Nitric oxide negatively regulates AKT1-mediated potassium uptake through modulating vitamin B6 homeostasis in Arabidopsis

Xia

Kong

Xue

et al. 2014

Proc. Natl. Acad. Sci. U.S.A.

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“…Recently, the role of NO in the regulation of stomatal closure has been well documented, and a growing body of literature shows that the potential enzymatic sources of NO in guard cells are nitric oxide synthase (NOS) and NR (Neill et al, 2002Qiao and Fan, 2008). However, so far, no genes or proteins with sequence homology to known mammalian-type NOS have been found in plants (Guo et al, 2003;Crawford, 2006).…”

Section: Nia1-dependent No Mediates Uv-b-induced Stomatal Closurementioning

confidence: 99%

“…Recently, compelling evidence emerged that hydrogen peroxide (H 2 O 2 ) and nitric oxide (NO) function as signaling molecules in plants, mediating a range of responses to environmental stress including UV-B radiation (Neill et al, 2002;Qiao and Fan, 2008;Wilson et al, 2008). Increasing evidence also points to the role for H 2 O 2 and NO as essential components in guard cell signaling.…”

mentioning

confidence: 99%

Role and Interrelationship of Gα Protein, Hydrogen Peroxide, and Nitric Oxide in Ultraviolet B-Induced Stomatal Closure in Arabidopsis Leaves

Zhang

et al. 2013

Plant Physiology

127

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Heterotrimeric G proteins have been shown to transmit ultraviolet B (UV-B) signals in mammalian cells, but whether they also transmit UV-B signals in plant cells is not clear. In this paper, we report that 0.5 W m 22 UV-B induces stomatal closure in Arabidopsis (Arabidopsis thaliana) by eliciting a cascade of intracellular signaling events including Ga protein, hydrogen peroxide (H 2 O 2 ), and nitric oxide (NO). UV-B triggered a significant increase in H 2 O 2 or NO levels associated with stomatal closure in the wild type, but these effects were abolished in the single and double mutants of AtrbohD and AtrbohF or in the Nia1 mutants, respectively. Furthermore, we found that UV-B-mediated H 2 O 2 and NO generation are regulated by GPA1, the Ga-subunit of heterotrimeric G proteins. UV-B-dependent H 2 O 2 and NO accumulation were nullified in gpa1 knockout mutants but enhanced by overexpression of a constitutively active form of GPA1 (cGa). In addition, exogenously applied H 2 O 2 or NO rescued the defect in UV-B-mediated stomatal closure in gpa1 mutants, whereas cGa AtrbohD/AtrbohF and cGa nia1 constructs exhibited a similar response to AtrbohD/ AtrbohF and Nia1, respectively. Finally, we demonstrated that Ga activation of NO production depends on H 2 O 2 . The mutants of AtrbohD and AtrbohF had impaired NO generation in response to UV-B, but UV-B-induced H 2 O 2 accumulation was not impaired in Nia1. Moreover, exogenously applied NO rescued the defect in UV-B-mediated stomatal closure in the mutants of AtrbohD and AtrbohF. These findings establish a signaling pathway leading to UV-B-induced stomatal closure that involves GPA1-dependent activation of H 2 O 2 production and subsequent Nia1-dependent NO accumulation.

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“…Nitric oxide controls a signaling cascade that regulates plant responses to developmental processes, biotic and abiotic stress 48, 49, 50, 51. We have found induced expression in the AdZADH2 transcripts at late stage of SNP treatment.…”

Section: Resultsmentioning

confidence: 63%

A novel zinc‐binding alcohol dehydrogenase 2 from Arachis diogoi, expressed in resistance responses against late leaf spot pathogen, induces cell death when transexpressed in tobacco

et al. 2016

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A novel zinc‐binding alcohol dehydrogenase 2 (AdZADH2) was significantly upregulated in a wild peanut, Arachis diogoi treated with conidia of late leaf spot (LLS) pathogen, Phaeoisariopsis personata. This upregulation was not observed in a comparative analysis of cultivated peanut, which is highly susceptible to LLS. This zinc‐binding alcohol dehydrogenase possessed a Rossmann fold containing NADB domain in addition to the MDR domain present in all previously characterized plant ADH genes/proteins. Transient over‐expression of AdZADH2 under an estradiol inducible promoter (XVE) resulted in hypersensitive response (HR)‐like cell death in tobacco leaf. However, the same level of cell death was not observed when the domains were transiently expressed individually. Cell death observed in tobacco was associated with overexpression of cell death related proteins, antioxidative enzymes such as SOD, CAT and APX and pathogenesis‐related (PR) proteins. In A. diogoi, AdZADH2 expression was significantly upregulated in response to the plant signaling hormones salicylic acid, methyl jasmonate, and sodium nitroprusside.

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Nitric Oxide Signaling in Plant Responses to Abiotic Stresses

Cited by 173 publications

References 107 publications

Nitric oxide negatively regulates AKT1-mediated potassium uptake through modulating vitamin B6 homeostasis in Arabidopsis

Nitric oxide negatively regulates AKT1-mediated potassium uptake through modulating vitamin B6 homeostasis in Arabidopsis

Role and Interrelationship of Gα Protein, Hydrogen Peroxide, and Nitric Oxide in Ultraviolet B-Induced Stomatal Closure in Arabidopsis Leaves

A novel zinc‐binding alcohol dehydrogenase 2 from Arachis diogoi, expressed in resistance responses against late leaf spot pathogen, induces cell death when transexpressed in tobacco

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