Nitrifying biomass characterization and monitoring during bioaugmentation in a membrane bioreactor

D'Anteo, Sibilla; Mannucci, Alberto; Meliani, Matteo; Verni, Franco; Petroni, Giulio; Munz, Giulio; Lubello, Claudio; Mori, Greg; Vannini, Claudia

doi:10.1080/09593330.2015.1055818

Cited by 8 publications

(2 citation statements)

References 30 publications

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“…Bdellovibrio" are poorly characterized isolates and environmental 16S rRNA sequences from diverse environments. These environments include commercial aquaculture preparations, soils, wastewater-activated sludge, and iron-oxidizing freshwater environments (7,(16)(17)(18). This broad diversity of habitats suggests that Bdellovibrio species that are closely related to "Ca.…”

Section: Discussionmentioning

confidence: 99%

From the Inside Out: an Epibiotic Bdellovibrio Predator with an Expanded Genomic Complement

Deeg

Zimmer

et al. 2020

J Bacteriol

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Bdellovibrio and like organisms are abundant environmental parasitoids of prokaryotes that show diverse predation strategies. The vast majority of studied Bdellovibrio bacteria and like organisms deploy intraperiplasmic replication inside the prey cell, while few isolates with smaller genomes consume their prey from the outside in an epibiotic manner. The novel parasitoid “Candidatus Bdellovibrio qaytius” was isolated from a eutrophic freshwater pond in British Columbia, where it was a continual part of the microbial community. “Ca. Bdellovibrio qaytius” was found to preferentially prey on the betaproteobacterium Paraburkholderia fungorum without entering the periplasm. Despite its epibiotic replication strategy, “Ca. Bdellovibrio” encodes a large genomic complement more similar to that of complex periplasmic predators. Functional genomic annotation further revealed several biosynthesis pathways not previously found in epibiotic predators, indicating that “Ca. Bdellovibrio” represents an intermediate phenotype and at the same time narrowing down the genomic complement specific to epibiotic predators. In phylogenetic analysis, “Ca. Bdellovibrio qaytius” occupies a widely distributed, but poorly characterized, basal cluster within the genus Bdellovibrio. This suggests that epibiotic predation might be a common predation type in nature and that epibiotic predation could be the ancestral predation type in the genus. IMPORTANCE Bdellovibrio and like organisms are bacteria that prey on other bacteria and are widespread in the environment. Most of the known Bdellovibrio species enter the space between the inner and outer prey membrane, where they consume their prey cells. However, one Bdellovibrio species has been described that consumes its prey from the outside. Here, we describe “Ca. Bdellovibrio qaytius,” a novel member of the genus Bdellovibrio that also remains outside the prey cell throughout its replication cycle. Unexpectedly, the genome of “Ca. Bdellovibrio” is much more similar to the genomes of intracellular predators than to the species with a similar life cycle. Since “Ca. Bdellovibrio” is also a basal representative of this genus, we hypothesize that extracellular predation could be the ancestral predation strategy.

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Section: Discussionmentioning

confidence: 99%

From the Inside Out: an Epibiotic Bdellovibrio Predator with an Expanded Genomic Complement

Deeg

Zimmer

et al. 2020

J Bacteriol

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“…Plasmid isolation was carried out using a commercial plasmid isolation kit (ZR Plasmid Miniprep Classic, USA) and the process was carried out according to the manufacturer instructions as described by D'Anteo [15]. Isolated plasmids were thereafter electrophoresed in a horizontal tank at a constant voltage of 90V for 60 minutes.…”

Section: Plasmid Dna Extraction and Gel Electrophoresismentioning

confidence: 99%

Genetic basis of multiple drug resistant Escherichia coli from urine samples in Ikpoba Okha Local Government Area of Edo State, Nigeria

Eremwanarue

Ehiaghe²

2018

GSC Biol. and Pharm. Sci.

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This study was aimed at characterizing bacteria at the molecular level and determining the genetic basis of the multidrug resistant bacteria isolates. Bacteria were isolated from eighty urine samples from urinary tract infection patients. Phenotypically identified isolates of Escherichia coli were selected. Multiple drug resistant (MDR) bacteria were generated from the isolates by carrying out antibiotic susceptibility tests using the Kirby-Bauer disc diffusion technique. MDR bacteria were selected and molecular characterization using polymerase chain reaction technique with species-specific primers was performed for confirming microbial identity. Plasmid DNA profiling was carried out to detect MDR Escherichia coli with plasmid and subsequent plasmid curing of isolates. The polymerase chain reaction result showed that all twenty isolates were Escherichia coli, fourteen out of the twenty isolates were multiple drug resistant Escherichia coli. The gel electrophoresis indicated that eleven out of the fourteen multiple drug resistant Escherichia coli contains plasmids with a molecular weight of 48.5kb. The E. coli isolates that habors plasmid were cured of the plasmids and the results of the antibiotics sensitivity tests showed that E. coli that showed resistance before curing became sensitive after curing. This study has shown that multidrug resistant E. coli are plasmid mediated in this locality.

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An Efficient Way for Nitrifying Bacteria Enrichment with Coal Ash: Nitrification and Microbial Community

Liu

Zhao

et al. 2017

Water Air Soil Pollut

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Nitrifying biomass characterization and monitoring during bioaugmentation in a membrane bioreactor

Cited by 8 publications

References 30 publications

From the Inside Out: an Epibiotic Bdellovibrio Predator with an Expanded Genomic Complement

From the Inside Out: an Epibiotic Bdellovibrio Predator with an Expanded Genomic Complement

Genetic basis of multiple drug resistant Escherichia coli from urine samples in Ikpoba Okha Local Government Area of Edo State, Nigeria

An Efficient Way for Nitrifying Bacteria Enrichment with Coal Ash: Nitrification and Microbial Community

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