Nitrile hydratase from Mesorhizobium sp. F28 and its potential for nitrile biotransformation

Feng, Yun-Shu; Chen, Pei-Chung; Wen, Fu-Shyan; Hsiao, Wan-Yi; Lee, Chi-Mei

doi:10.1016/j.procbio.2008.08.009

Cited by 30 publications

(15 citation statements)

References 39 publications

(34 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Although the kinetic parameters of the ANHase toward various nitriles have been extensively reported, 33 parameters for the neonicotinoid insecticide AAP degradation have rarely been cited. As shown in Fig.…”

Section: Lc-ms Analysis Indicated That the Metabolite Displayed A Promentioning

confidence: 99%

Biodegradation of the neonicotinoid insecticide acetamiprid in surface water by the bacterium Variovorax boronicumulans CGMCC 4969 and its enzymatic mechanism

et al. 2017

View full text Add to dashboard Cite

show abstract

Section: Lc-ms Analysis Indicated That the Metabolite Displayed A Promentioning

confidence: 99%

Biodegradation of the neonicotinoid insecticide acetamiprid in surface water by the bacterium Variovorax boronicumulans CGMCC 4969 and its enzymatic mechanism

et al. 2017

View full text Add to dashboard Cite

show abstract

“…Nitrilase of Mesorhizobium sp. F28 was inhibited about 64% by EDTA (Feng et al 2008), while nitrilases from R. rhodochrous J1 and B . pallidus Dac521 were unaffected by 1 mM and 10 mM EDTA, respectively (Nagasawa et al 1993;Cramp et al 1999).…”

Section: Effect Of Different Ion Sources On Nitrilase Productionmentioning

confidence: 94%

“…The enzyme activity was about 30% of the maximum at 60 ° C. The optimal temperature of the Mesorhizobium sp. F28 nitrilase was 37 -45 ° C, but this enzyme was inactive at 55 -65 ° C (Feng et al 2008). Nageshwar and coworkers reported that the nitrilase activity of A. faecalis MTCC 10757 was maximal at 35 ° C by A. faecalis MTCC 10757 (Nageshwar et al 2011).…”

Section: Effect Of Different Ion Sources On Nitrilase Productionmentioning

confidence: 98%

“…NCIB 11216 nitrilase was also inhibited by Fe 2 ϩ and Mn 2 ϩ , while Feng and coworkers (Feng et al 2008) reported that Mesorhizobium sp. F28 was sensitive to CuSO 4 and Ag 2 SO 3 , which both react with sulfhydryl groups.…”

Section: Effect Of Different Ion Sources On Nitrilase Productionmentioning

confidence: 99%

“…(He et al 2007(He et al , 2010, Bradyrhizobium japonicum (Zhu et al 2008), Arthrobacter nitroguajacolicus (Mei et al 2009), Serratia marcescens (Jin et al 2010), Mesorhizobium sp. F28 (Feng et al 2008), Pseudomonas sp. (Komeda et al 2004;Kim et al 2009;Kiziak and Stolz 2009;Kumar et al 2010), Geotrichum sp.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Nitrile-metabolizing potential ofBacilluscereus strain FA12; Nitrilase production, purification, and characterization

Ramezani-pour

Badoei-dalfard

Namaki-Shoushtari

et al. 2015

Biocatalysis and Biotransformation

View full text Add to dashboard Cite

Nitrile-hydrolyzing bacteria have the potential to perform useful biotransformations such as the production of industrially useful acids and amides. In this study, we report a nitrile-degrading bacterium with signifi cant nitrile metabolism. Molecular characterization of 16S rDNA gene characterized this strain as Bacillus cereus . Medium optimization of B. cereus FA12 showed that biomass and nitrilase production was strongly supported by glucose (10 gL Ϫ 1 ) and yeast extract (10 gL Ϫ 1 ). Enzymatic production improved slightly in the pH range from 6.0 to 7.0. The addition of Mg ϩ 2 , Fe ϩ 2 , and Na ϩ supported biomass and nitrilase production; however, other metal ions, Co ϩ 2 and Cu ϩ 2 , inhibited production. The apparent molecular mass of the puri fi ed FA12 nitrilase as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) was about 45 kDa. Nitrilase FA12 shows relatively high activity and stability at pH 7.0 and 40 ° C. Nitrilase FA12 was marginally inhibited with Ca ϩ 2 and Co ϩ 2 , whereas inhibition in the presence of dithiothreitol or DTT was 80%. The pseudo K m (mM) values of resting cells (i.e., treating whole cells as if they were an enzyme) for acetonitrile and acetamide were determined to be 2.36 and 1.81, respectively. Under optimum situations, B. cereus FA12 resting cells produced 83 and 58 (U/mg) acetonitrile/acetamide degrading activity, respectively. Ammonia production from acetamide and acetonitrile by the B. cereus FA12 was maximum after 5 and 7 h of incubation, respectively. These results indicate that B. cereus FA12 resting cells may be used in nitrile biotransformations to produce commercially useful compounds.

show abstract

Key‐Study on the Kinetic Aspects of theIn SituNHase/AMase Cascade System ofM. imperialeResting Cells for Nitrile Bioconversion

Cantarella

Pasquarelli

Spera

et al. 2014

Cascade Biocatalysis

View full text Add to dashboard Cite

Nitrile hydratase from Mesorhizobium sp. F28 and its potential for nitrile biotransformation

Cited by 30 publications

References 39 publications

Biodegradation of the neonicotinoid insecticide acetamiprid in surface water by the bacterium Variovorax boronicumulans CGMCC 4969 and its enzymatic mechanism

Biodegradation of the neonicotinoid insecticide acetamiprid in surface water by the bacterium Variovorax boronicumulans CGMCC 4969 and its enzymatic mechanism

Nitrile-metabolizing potential ofBacilluscereus strain FA12; Nitrilase production, purification, and characterization

Key‐Study on the Kinetic Aspects of theIn SituNHase/AMase Cascade System ofM. imperialeResting Cells for Nitrile Bioconversion

Contact Info

Product

Resources

About