Nitriliruptor alkaliphilus gen. nov., sp. nov., a deep-lineage haloalkaliphilic actinobacterium from soda lakes capable of growth on aliphatic nitriles, and proposal of Nitriliruptoraceae fam. nov. and Nitriliruptorales ord. nov.

Sorokin, Dimitry Y.; Pelt, Sander van; Tourova, T. P.; Evtushenko, Lyudmila I.

doi:10.1099/ijs.0.002204-0

Cited by 102 publications

(71 citation statements)

References 19 publications

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“…In the present dataset, the dominant genera of the order Nitriliruptoridae were Nitriliruptor and Euzebya. Nitriliruptor is a deep-branching haloalkaliphilic actinobacterium isolated from soda lakes and is capable of growth on aliphatic nitriles (Sorokin et al 2009). The genus Euzebya is a deepbranching marine actinobacterium isolated from sea cucumbers (Kurahashi et al 2010).…”

Section: Bacteriamentioning

confidence: 99%

A meta-analysis of the publicly available bacterial and archaeal sequence diversity in saline soils

Gong

2013

World J Microbiol Biotechnol

102

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An integrated view of bacterial and archaeal diversity in saline soil habitats is essential for understanding the biological and ecological processes and exploiting potential of microbial resources from such environments. This study examined the collective bacterial and archaeal diversity in saline soils using a meta-analysis approach. All available 16S rDNA sequences recovered from saline soils were retrieved from publicly available databases and subjected to phylogenetic and statistical analyses. A total of 9,043 bacterial and 1,039 archaeal sequences, each longer than 250 bp, were examined. The bacterial sequences were assigned into 5,784 operational taxonomic units (OTUs, based on C97 % sequence identity), representing 24 known bacterial phyla, with Proteobacteria (44.9 %), Actinobacteria (12.3 %), Firmicutes (10.4 %), Acidobacteria (9.0 %), Bacteroidetes (6.8 %), and Chloroflexi (5.9 %) being predominant. Lysobacter (12.8 %) was the dominant bacterial genus in saline soils, followed by Sphingomonas (4.5 %), Halomonas (2.5 %), and Gemmatimonas (2.5 %). Archaeal sequences were assigned to 602 OTUs, primarily from the phyla Euryarchaeota (88.7 %) and Crenarchaeota (11.3 %).Halorubrum and Thermofilum were the dominant archaeal genera in saline soils. Rarefaction analysis indicated that less than 25 % of bacterial diversity, and approximately 50 % of archaeal diversity, in saline soil habitats has been sampled. This analysis of the global bacterial and archaeal diversity in saline soil habitats can guide future studies to further examine the microbial diversity of saline soils.

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Section: Bacteriamentioning

confidence: 99%

A meta-analysis of the publicly available bacterial and archaeal sequence diversity in saline soils

Gong

2013

World J Microbiol Biotechnol

102

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“…With the aims of keeping consistency with the rank of other prokaryotes and developing a unified classification across all bacteria and archaea, the class Actinobacteria containing five subclasses (Goodfellow & distinct phyletic lineage and signature nucleotides of 16S rRNA gene sequence, as well as other characteristics, such as Gram-staining-positive rods and the cell-wall peptidoglycan A1c type (Sorokin et al, 2009;Kurahashi et al, 2010).…”

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confidence: 99%

“…T contained meso-diaminopimelic acid, alanine and glutamic acid as major amino acids, which indicated the presence of the A1c type peptidoglycan (Schleifer & Kandler, 1972;Sorokin et al, 2009;Kurahashi et al, 2010). Strain EGI 80759…”

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confidence: 99%

“…The phylogenetic relationship (Fig. 2) and signature nucleotides of 16S rRNA gene sequences (see the family description) between strains EGI 80759 T and N. alkaliphilus ANL-iso2 T (Sorokin et al, 2009) also differs greatly, thereby indicating that strain EGI 80759 T does not fall into the order Nitriliruptorales. In conclusion, strain EGI 80759 T does not belong to any order of the class Nitriliruptoria.…”

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Egibacter rhizosphaerae gen. nov., sp. nov., an obligately halophilic, facultatively alkaliphilic actinobacterium and proposal of Egibaceraceae fam. nov. and Egibacterales ord. nov.

Zhang

Wang

Yang

et al. 2016

International Journal of Systematic and Evolutionary Microbiology

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Egibacter rhizosphaerae gen. nov., sp. nov., an obligately halophilic, facultatively alkaliphilic actinobacterium and proposal of Egibaceraceae fam. nov. and Egibacterales ord. nov. T were Gram-stain-positive, non-motile and non-endospore-forming rods. Strain EGI 80759 T showed obligately halophilic growth with a tolerance to 8-25 % (w/v) NaCl (optimum growth at 10-12 %, w/v) and facultatively alkaliphilic growth within the pH range 7.0-11.0 (optimum growth at pH 9.0-10.0). Cell-wall hydrolysates of the isolate contained mesodiaminopimelic acid (peptidoglycan type A1c), with glucose, glucosamine, ribose and mannose as the major sugars. The major fatty acids identified were 10-methyl-C 17 : 0 , C 17 : 1 v8c and C 17 : 0 . The predominant menaquinone was MK-9(H 4 ). The G+C content of the genomic DNA was 72.1 mol%. Phylogenetic analysis, based on 16S rRNA gene sequences, revealed that strain EGI 80759 T clustered with members of the class Nitriliruptoria and showed highest 16S rRNA gene sequence similarities with Euzebya tangerina F10 T (90.3 %) and Nitriliruptor alkaliphilus ANL-iso2

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“…Nitriliruptoraceae are haloalkaliphic bacteria that require high salt levels to decompose organic C containing nitrile groups (Sorokin et al, 2009). The activity of abundant 490 freshwater taxa may relate to differences in C source availability.…”

Section: Abundant Taxa Are Disproportionately Active 474mentioning

confidence: 99%

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Extremophiles employ a diverse array of resistance strategies to thrive under harsh 18 environmental conditions but maintaining these adaptations comes at an energetic cost. If energy reserves to drop too low, extremophiles may enter a dormant state of reduced 20 metabolic activity to survive. Dormancy is frequently offered as a plausible explanation for the persistence of bacteria under suboptimal environmental conditions with the 22 prevalence of this mechanism only expected to rise as stressful conditions intensify. We estimated dormancy in ten hypersaline and freshwater lakes across the Western United 24States. To our surprise, we found that extreme environmental conditions did not induce higher levels of bacterial dormancy. Based on our approach using rRNA:rDNA gene 26 ratios to estimate activity, halophilic and halotolerant bacteria were classified as inactive at a similar percentage as freshwater bacteria, and the proportion of the community 28 exhibiting dormancy was considerably lower (16%) in hypersaline than freshwater lakes across a range of cutoffs estimating activity. Of the multiple chemical characteristics we 30 evaluated, salinity and, to a lesser extent, total phosphorus concentrations influenced activity. But instead of dormancy being more common as stressful conditions intensified, 32 the percentage of the community residing in an inactive state decreased with increasing salinity in freshwater and hypersaline lakes, suggesting that salinity acts as a strong 34 environmental filter selecting for bacteria that persist and thrive under saltier conditions. Within the compositionally distinct and less diverse hypersaline communities, abundant 36 taxa were disproportionately active and localized in families Microbacteriaceae

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Nitriliruptor alkaliphilus gen. nov., sp. nov., a deep-lineage haloalkaliphilic actinobacterium from soda lakes capable of growth on aliphatic nitriles, and proposal of Nitriliruptoraceae fam. nov. and Nitriliruptorales ord. nov.

Cited by 102 publications

References 19 publications

A meta-analysis of the publicly available bacterial and archaeal sequence diversity in saline soils

A meta-analysis of the publicly available bacterial and archaeal sequence diversity in saline soils

Egibacter rhizosphaerae gen. nov., sp. nov., an obligately halophilic, facultatively alkaliphilic actinobacterium and proposal of Egibaceraceae fam. nov. and Egibacterales ord. nov.

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