Nitrite‐dependent nitric oxide synthesis by molybdenum enzymes

Bender, Daniel A.; Schwarz, Günter

doi:10.1002/1873-3468.13089

Cited by 54 publications

(54 citation statements)

References 132 publications

(251 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Our results indicate that in plants with a single NiR, nitrite reduction to ammonium is likely a key step not only for proper nitrogen assimilation but also for NO production, both factors being relevant for determining plant growth rates. NO has been also extensively characterized as a regulator of plant responses to abiotic and biotic stress factors [ 8 ]. Therefore, the altered function of NiR1 in Arabidopsis and in plants with single NiR may have also an impact on plant defense to stress.…”

Section: Discussionmentioning

confidence: 99%

“…This reductive assimilatory pathway thus involves different subcellular locations as it begins in the cytoplasm but ends in chloroplasts. However, nitrite, instead of being reduced to ammonium in plastids, can be reduced to nitric oxide (NO) in mitochondria, through a process that is relevant under hypoxic conditions [ 6 ], or in the cytoplasm, through a side-reaction catalyzed by NRs [ 7 , 8 ]. The balance between redox reactions catalyzed by NR and NiR then seems crucial to determine the amount of NO that plants can produce and accumulate.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Nitrite Reductase 1 Is a Target of Nitric Oxide-Mediated Post-Translational Modifications and Controls Nitrogen Flux and Growth in Arabidopsis

Costa-Broseta

Castillo

León

2020

IJMS

View full text Add to dashboard Cite

Plant growth is the result of the coordinated photosynthesis-mediated assimilation of oxidized forms of C, N and S. Nitrate is the predominant N source in soils and its reductive assimilation requires the successive activities of soluble cytosolic NADH-nitrate reductases (NR) and plastid stroma ferredoxin-nitrite reductases (NiR) allowing the conversion of nitrate to nitrite and then to ammonium. However, nitrite, instead of being reduced to ammonium in plastids, can be reduced to nitric oxide (NO) in mitochondria, through a process that is relevant under hypoxic conditions, or in the cytoplasm, through a side-reaction catalyzed by NRs. We use a loss-of-function approach, based on CRISPR/Cas9-mediated genetic edition, and gain-of-function, using transgenic overexpressing HA-tagged Arabidopsis NiR1 to characterize the role of this enzyme in controlling plant growth, and to propose that the NO-related post-translational modifications, by S-nitrosylation of key C residues, might inactivate NiR1 under stress conditions. NiR1 seems to be a key target in regulating nitrogen assimilation and NO homeostasis, being relevant to the control of both plant growth and performance under stress conditions. Because most higher plants including crops have a single NiR, the modulation of its function might represent a relevant target for agrobiotechnological purposes.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Nitrite Reductase 1 Is a Target of Nitric Oxide-Mediated Post-Translational Modifications and Controls Nitrogen Flux and Growth in Arabidopsis

Costa-Broseta

Castillo

León

2020

IJMS

View full text Add to dashboard Cite

show abstract

“…Humans are characterized by a single AOX enzyme, while rodents express four isoenzymes (AOX1-4). Both enzymes AOX and XOR are able to generate besides ROS also nitric oxide (NO) (see Maia and Moura 2018;Bender and Schwarz 2018). Under normal culturing conditions of adipocytes, AOX localizes in the cytoplasm of cells ( Fig.…”

Section: Aox Is Expressed In Adipocytes and Links Lds And Mitochondrimentioning

confidence: 99%

Formation and degradation of lipid droplets in human adipocytes and the expression of aldehyde oxidase (AOX)

et al. 2019

View full text Add to dashboard Cite

Lipid droplet (LD) binding proteins in mammary glands and in adipocytes were previously compared and striking similar sets of these specific proteins demonstrated. Xanthine oxidoreductase (XOR) together with perilipins and the lactating mammary gland protein butyrophilin play an important role in the secretion process of LDs into milk ducts. In contrast, in adipose tissue and in adipocytes, mainly perilipins have been described. Moreover, XOR was reported in mouse adipose tissue and adipocyte culture cells as "novel regulator of adipogenesis". This obvious coincidence of protein sets prompted us to revisit the formation of LDs in human-cultured adipocytes in more detail with special emphasis on the possibility of a LD association of XOR. We demonstrate by electron and immunoelectron microscopy new structural details on LD formation in adipocytes. Surprisingly, by immunological and proteomic analysis, we identify in contrast to previous data showing the enzyme XOR, predominantly the expression of aldehyde oxidase (AOX). AOX could be detected tightly linked to LDs when adipocytes were treated with starvation medium. In addition, the majority of cells show an enormous interconnected, tubulated mitochondria network. Here, we discuss that (1) XOR is involved-together with perilipins-in the secretion of LDs in alveolar epithelial cells of the lactating mammary gland and is important in the transcytosis pathway of capillary endothelial cells. (2) In cells, where LDs are not secreted, XOR cannot be detected at the protein level, whereas in contrast in these cases, AOX is often present. We detect AOX in adipocytes together with perilipins and find evidence that these proteins might direct LDs to mitochondria. Finally, we here report for the first time the exclusive and complementary localization of XOR and AOX in diverse cell types.

show abstract

“…Recent years, molybdenum oxide nanomaterials have become a popular nanomaterial in different areas, including energy storage, [13,14] catalysis, [15][16][17] chem/bio-sensing [18][19][20][21][22][23] and biomedicine. [24][25][26][27] However, the catalysis property especially the bio-catalysis behavior of molybdenum oxide nanomaterials is little understood, even though molybdenum has been employed as a key cofactor (Moco) in several important enzymes in biological system, such as xanthine oxidase, [28] nitrate reductase, [29] and sulfite oxidase. [30] For instance, Tremel and coworkers reported that molybdenum trioxide nanoparticles have an intrinsic biomimetic sulfite oxidase activity, and Yang etc found that MoO 3-x nanodots have excellent catalase and SOD mimic activities.…”

Section: Introductionmentioning

confidence: 99%

Peroxidase‐Mimetic and Fenton‐Like Activities of Molybdenum Oxide Quantum Dots

et al. 2020

View full text Add to dashboard Cite

Molybdenum oxide nanomaterials have become a popular nanomaterial while its catalysis behavior especially the bio‐catalysis property is little understood, even though molybdenum has been employed as a key cofactor in several important enzymes in biological system. On the basis of these, the Fenton and peroxidase‐like property of molybdenum oxide quantum dots (MoOx QDs) with mixed valence state was carefully investigated using tetramethylbenzidine (TMB) and methyl orange (MO) as the substrates. The results showed that MoOx QDs not only serve as a Fenton reagent, degrading MO to colorless products, but also exhibit intrinsic peroxidase‐mimetic activity, oxidizing TMB to the oxidized state oxTMB. The catalysis mechanism of MoOx QDs is that MoOx QDs can facilitate the decomposition of H2O2 and a large amount of radicals was generated, leading to MO decomposing and TMB oxidizing subsequently. Inspired by the findings, a visible method was developed for sensitive and selective detection of H2O2 and glucose, and the limit of detection for H2O2 and glucose is 7.75 μM and 8.95 μM (3σ/K), respectively. Moreover, glucose content in human serum samples was successfully determined by the proposed visible method, and the results are consistent well with those obtained by handheld glucose meter, suggesting a high accuracy of the present method.

show abstract

Nitrite‐dependent nitric oxide synthesis by molybdenum enzymes

Cited by 54 publications

References 132 publications

Nitrite Reductase 1 Is a Target of Nitric Oxide-Mediated Post-Translational Modifications and Controls Nitrogen Flux and Growth in Arabidopsis

Nitrite Reductase 1 Is a Target of Nitric Oxide-Mediated Post-Translational Modifications and Controls Nitrogen Flux and Growth in Arabidopsis

Formation and degradation of lipid droplets in human adipocytes and the expression of aldehyde oxidase (AOX)

Peroxidase‐Mimetic and Fenton‐Like Activities of Molybdenum Oxide Quantum Dots

Contact Info

Product

Resources

About