Nitroaromatic Actuation of Mitochondrial Bioelectrocatalysis for Self-Powered Explosive Sensors

Germain, Marguerite N.; Arechederra, Robert L.

doi:10.1021/ja807250b

Cited by 65 publications

(52 citation statements)

References 14 publications

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“…This modified Nafion polymer has been used to immobilize many different types of enzymes as well as mitochondria for use in biosensors and biofuel cells. [8][9][10][11][12] This paper describes a novel procedure for making this micellar polymer enzyme immobilization membrane that can stabilize enzymes. The synthesis of the micellar enzyme immobilization membrane, the procedure for immobilizing enzymes within the membrane, and the assays for studying enzymatic specific activity of the immobilized enzyme are detailed below.…”

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confidence: 99%

Hydrophobic Salt-modified Nafion for Enzyme Immobilization and Stabilization

Meredith

2012

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Over the last decade, there has been a wealth of application for immobilized and stabilized enzymes including biocatalysis, biosensors, and biofuel cells. [1][2][3] In most bioelectrochemical applications, enzymes or organelles are immobilized onto an electrode surface with the use of some type of polymer matrix. This polymer scaffold should keep the enzymes stable and allow for the facile diffusion of molecules and ions in and out of the matrix. Most polymers used for this type of immobilization are based on polyamines or polyalcohols -polymers that mimic the natural environment of the enzymes that they encapsulate and stabilize the enzyme through hydrogen or ionic bonding. Another method for stabilizing enzymes involves the use of micelles, which contain hydrophobic regions that can encapsulate and stabilize enzymes. 4,5 In particular, the Minteer group has developed a micellar polymer based on commercially available Nafion. 6,7 Nafion itself is a micellar polymer that allows for the channel-assisted diffusion of protons and other small cations, but the micelles and channels are extremely small and the polymer is very acidic due to sulfonic acid side chains, which is unfavorable for enzyme immobilization. However, when Nafion is mixed with an excess of hydrophobic alkyl ammonium salts such as tetrabutylammonium bromide (TBAB), the quaternary ammonium cations replace the protons and become the counter ions to the sulfonate groups on the polymer side chains (Figure 1). This results in larger micelles and channels within the polymer that allow for the diffusion of large substrates and ions that are necessary for enzymatic function such as nicotinamide adenine dinucleotide (NAD). This modified Nafion polymer has been used to immobilize many different types of enzymes as well as mitochondria for use in biosensors and biofuel cells. [8][9][10][11][12] This paper describes a novel procedure for making this micellar polymer enzyme immobilization membrane that can stabilize enzymes. The synthesis of the micellar enzyme immobilization membrane, the procedure for immobilizing enzymes within the membrane, and the assays for studying enzymatic specific activity of the immobilized enzyme are detailed below. Video LinkThe video component of this article can be found at

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Hydrophobic Salt-modified Nafion for Enzyme Immobilization and Stabilization

Meredith

2012

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“…[11][12][13][14] Recently, by theu se of inhibitor and decoupler effects, BFCbased self-powered sensing conceptsh ave also be explored to detecto ther important analytes such as cyanide, ethylene diaminet etraacetic acid (EDTA) and explosive compounds. [4,9,10] These BFC-based sensors open the way for future point-of-care testinga nd might provide ah elpfuls olution to low-cost and simplea nalytical systems without the need of any additional externale nergy.T od ate, the studies on BFC-baseds ensors mainly focus on their long-term operation, miniaturization, and extendt he analytes scope. However,afew reports are focused on regulating/ort uning electrocatalytic processes at the electrode surface of BFCs, [15,16] whichi st he most fundamental factorf or the development of BFC-based biosensors.…”

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confidence: 99%

Rational Tuning of the Electrocatalytic Nanobiointerface for a “Turn‐Off” Biofuel‐Cell‐Based Self‐Powered Biosensor for p53 Protein

Han

Chabu

et al. 2015

Chemistry A European J

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Herein, a novel tunable electrocatalytic nanobiointerface for the construction of a high-sensitivity and high-selectivity biofuel-cell (BFC)-based self-powered biosensor for the detection of transcription factor protein p53 is reported, in which bilirubin oxidase (BOD)/DNA supramolecular modified graphene/platinum nanoparticles hybrid nanosheet (GPNHN) works as a new enhanced material of biocathode to control the attachment of target, and thus tune the electron-transfer process of oxygen reduction for transducing signaling magnification. It is found that in the presence of p53, the strong interaction between the wild-type p53 and its consensus DNA sequence on the electrode surface can block the electron transfer from the BOD to the electrode, thus providing a good opportunity for reducing the electrocatalytic activity of oxygen reduction in the biocathode. This in combination with the glucose oxidation at the carbon nanotube/Meldola's blue/glucose dehydrogenase bioanode can result in a current/or power decrease of BFC in the presence of wild-type p53. The specially designed BFC-based self-powered p53 sensor shows a wide linear range from 1 pM to 1 μM with a detection limit of 1 pM for analyzing wild-type p53. Most importantly, our BFC-based self-powered sensors can detect the concentrations of wild-type p53 in normal and cancer cell lysates without any extensive sample pretreatment/separation or specialized instruments. The present BFC-based self-powered sensor can provide a simple, economical, sensitive, and rapid way for analyzing p53 protein in normal and cancer cells at clinical level, which shows great potential for creating the treatment modalities that capitalize on the concentration variation of the wild-type p53.

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“…Detection was possible by measuring the increase in power output with increasing fuel concentrations. Subsequently, the Minteer Research Group reported a self-powered nitroaromatic explosives sensor utilizing mitochondria for detection [9] as well as an enzymatic self-powered EDTA biosensor [10]. Another common detection method for self-powered biosensors takes advantage of enzymatic inhibitor effects.…”

Section: Introductionmentioning

confidence: 99%

Long-term arsenic monitoring with an Enterobacter cloacae microbial fuel cell

Rasmussen

2015

Bioelectrochemistry

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A microbial fuel cell was constructed with biofilms of Enterobacter cloacae grown on the anode. Bioelectrocatalysis was observed when the biofilm was grown in media containing sucrose as the carbon source and methylene blue as the mediator. The presence of arsenic caused a decrease in bioelectrocatalytic current. Biofilm growth in the presence of arsenic resulted in lower power outputs whereas addition of arsenic showed no immediate result in power output due to the short term arsenic resistance of the bacteria and slow transport of arsenic across cellular membranes to metabolic enzymes. Calibration curves plotted from the maximum current and maximum power of power curves after growth show that this system is able to quantify both arsenate and arsenate with low detection limits (46 μM for arsenate and 4.4 μM for arsenite). This system could be implemented as a method for long-term monitoring of arsenic concentration in environments where arsenic contamination could occur and alter the metabolism of the organisms resulting in a decrease in power output of the self-powered sensor.

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Nitroaromatic Actuation of Mitochondrial Bioelectrocatalysis for Self-Powered Explosive Sensors

Cited by 65 publications

References 14 publications

Hydrophobic Salt-modified Nafion for Enzyme Immobilization and Stabilization

Hydrophobic Salt-modified Nafion for Enzyme Immobilization and Stabilization

Rational Tuning of the Electrocatalytic Nanobiointerface for a “Turn‐Off” Biofuel‐Cell‐Based Self‐Powered Biosensor for p53 Protein

Long-term arsenic monitoring with an Enterobacter cloacae microbial fuel cell

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