Nitrocellulose Degradation by the Fungus Fusarium solani

Саратовских, Е. А.; Shcherbakova, V. A.; Yarullin, R. N.

doi:10.1134/s0003683818010106

Cited by 12 publications

(3 citation statements)

References 19 publications

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“…Agar is composed of carbon, hydrogen, oxygen, and sulfur (Bornman and Barnard 1993). Although the growth of the organisms on nitrocellulose alone is very slow (Saratovskikh et al 2018) and non-existent on the time scale of the experiment (data not shown), we propose that P. chrysosporium grows using the carbon and nitrogen sources either present in the treated miscanthus or small degradation products of the agar.…”

Section: )mentioning

confidence: 79%

Morphological growth pattern of Phanerochaete chrysosporium cultivated on different Miscanthus x giganteus biomass fractions

Khalil

Legin

Kurek

et al. 2020

Preprint

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The growth pattern of Phanerochaete chrysosporium on different Miscanthus x giganteus biomass fractions was investigated via an original method based on microscopy and image processing. The growth medium, together with image acquisition, was designed to study the mycelia growth non-invasively. Object coalescence, the colonized surface area, and radial expansion of the colony were measured. The substrate was sterilized by autoclaving, which could be considered a pre-treatment. The fastest growth rate was measured on the entire biomass, followed by the soluble fraction of the biomass, and finally, the residual solid fraction of the substrate pre-treated by autoclave. The growth rate on the different fractions of the substrate was additive, suggesting that both the solid and liquid fractions were used by the fungus, although the solid fraction of the substrate seemed to provide a richer but less accessible nutritional source. Based on the FTIR analysis, there were differences in composition between the solid and soluble fractions of the substrate, but the main components for growth were always present. We propose using this novel method for measuring initial fungal growth by following the variation of the number of objects over time. The methodology may be applied to the examination of other filamentous fungi.

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Section: )mentioning

confidence: 79%

Morphological growth pattern of Phanerochaete chrysosporium cultivated on different Miscanthus x giganteus biomass fractions

Khalil

Legin

Kurek

et al. 2020

Preprint

View full text Add to dashboard Cite

show abstract

“…Agar is composed of carbon, hydrogen, oxygen, and sulphur [ 98 ]. Although the growth of the organisms on nitrocellulose alone is very slow [ 99 ] and non-existent on the time scale of the experiment (data not shown), we propose that P. chrysosporium grows using the carbon and nitrogen sources either present in the treated miscanthus or small degradation products of the agar. Nitrogen content was suggested to play a crucial role in mediating the growth of P. chrysosporium .…”

Section: Discussionmentioning

confidence: 93%

Morphological growth pattern of Phanerochaete chrysosporium cultivated on different Miscanthus x giganteus biomass fractions

et al. 2021

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Background Solid-state fermentation is a fungal culture technique used to produce compounds and products of industrial interest. The growth behaviour of filamentous fungi on solid media is challenging to study due to the intermixity of the substrate and the growing organism. Several strategies are available to measure indirectly the fungal biomass during the fermentation such as following the biochemical production of mycelium-specific components or microscopic observation. The microscopic observation of the development of the mycelium, on lignocellulosic substrate, has not been reported. In this study, we set up an experimental protocol based on microscopy and image processing through which we investigated the growth pattern of Phanerochaete chrysosporium on different Miscanthus x giganteus biomass fractions. Results Object coalescence, the occupied surface area, and radial expansion of the colony were measured in time. The substrate was sterilized by autoclaving, which could be considered a type of pre-treatment. The fastest growth rate was measured on the unfractionated biomass, followed by the soluble fraction of the biomass, then the residual solid fractions. The growth rate on the different fractions of the substrate was additive, suggesting that both the solid and soluble fractions were used by the fungus. Based on the FTIR analysis, there were differences in composition between the solid and soluble fractions of the substrate, but the main components for growth were always present. We propose using this novel method for measuring the very initial fungal growth by following the variation of the number of objects over time. Once growth is established, the growth can be followed by measurement of the occupied surface by the mycelium. Conclusion Our data showed that the growth was affected from the very beginning by the nature of the substrate. The most extensive colonization of the surface was observed with the unfractionated substrate containing both soluble and solid components. The methodology was practical and may be applied to investigate the growth of other fungi, including the influence of environmental parameters on the fungal growth.

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“…The microbiological NC degradation with the sulfatereducing bacteria Desulfovibrio desulfuricans [19] and filamentous fungus Fusarium solani [24,25] has been studied, and the influence of the preliminary NC treatment on the process when UV-irradiation and ozone are used has been considered.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of denitration of nitrocellulose by microbiological treatment for industrial waste effluents using calorimetry analysis

Саратовских

Авдеева

Yarullin

et al. 2018

J Therm Anal Calorim

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The problem of utilization of the compounds present in the effluents of the production of cellulose nitrates (NC) is very important and complicated, and its decision is inextricably related to the environment. The process of heat release in the samples of nitrocellulose subjected to biological processing of different types was studied. Biological processing was conducted by incubation with sulfate-reducing bacteria Desulfovibrio desulfuricans BRMB-1388, microscopic fungi Fusarium solani BKM F-819, and their mixed culture. The studies were carried out on the native NC (13.38% nitrogen) and the NC processed (NC proc ) using ultraviolet radiation and ozone (UV ? ozone). It has been shown that the pretreatment of the NC by UV ? ozone significantly increases the degree of decomposition of the NC during its subsequent biodegradation assisted by symbiotic microorganisms of D. desulfuricans and F. solani. A substantial result was achieved on the fifth day of NC proc incubation, which is promising from the viewpoint of practical applications.

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Nitrocellulose Degradation by the Fungus Fusarium solani

Cited by 12 publications

References 19 publications

Morphological growth pattern of Phanerochaete chrysosporium cultivated on different Miscanthus x giganteus biomass fractions

Morphological growth pattern of Phanerochaete chrysosporium cultivated on different Miscanthus x giganteus biomass fractions

Morphological growth pattern of Phanerochaete chrysosporium cultivated on different Miscanthus x giganteus biomass fractions

Evaluation of denitration of nitrocellulose by microbiological treatment for industrial waste effluents using calorimetry analysis

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