Nitrogen Advanced Treatment of Urban Sewage by Denitrification Deep-Bed Filter: Removal Performance and Metabolic Pathway

Abstract

This study aimed to explore the performance of denitrification deep-bed filter (DN-DBF) to treat municipal sewage for meeting a more stringent discharge standard of total nitrogen (TN) (10.0 mg L–1). A lab-scale DN-DBF was conducted to optimize operation parameters and reveal the microbiological mechanism for TN removal. The results showed that more than 12.7% TN removal was obtained by adding methanol compared with sodium acetate. The effluent TN concentration reached 6.0–7.0 mg L–1 with the optimal influent carbon and nitrogen ratio (C/N) and hydraulic retention time (HRT) (3:1 and 0.25 h). For the nitrogen removal mechanism, Blastocatellaceae_Subgroup_4 and norank_o_JG30-KF-CM45 were dominant denitrification floras with an abundance of 6–10%. Though large TN was removed at the top layer of DN-DBF, microbial richness and diversity at the middle layer were higher than both ends. However, the relative abundance of nitrite reductase enzymes (EC1.7.2.1) gradually increases as the depth increases; conversely, the relative abundance of nitrous oxide reductase gradually decreased.

Food wastewater treatment using a hybrid biofilm reactor: nutrient removal performance and functional microorganisms on filler biofilm and suspended sludge

“… 38 The dominant genera in the fillers of column O, norank_f_JG30-KF-CM45 , are associated with COD degradation 39 and denitrification. 40 In the current study, the functional microorganisms in the filler biofilms of column O played greater roles in various metabolic pathways, especially carbohydrate and nitrogen metabolism. Hybrid fillers may form a micro-ecological environment that contributes to the development of different microbial species, thus improving FWW treatment efficiency.…”

Biochar Addition in Membrane Bioreactor Enables Membrane Fouling Alleviation and Nitrogen Removal Improvement for Low C/N Municipal Wastewater Treatment

“…The top 20 dominant genera in the CMBR and BMBR were displayed in a hierarchically clustered heatmap ( Figure 6 C). The microbial flora of norank_f__JG30-KF-CM45 [ 62 ] and Plasticucumulans were more abundant in BMBR than in CMBR, which was identified as denitrification bacteria [ 63 , 64 ]. The relative abundance of Plasticucumulans differed drastically, from 2.3% in CMBR to 3.5% in BMBR, and may lead to better TN removal in BMBR.…”

Application of Deep-bed Denitrification Filter in Sewage Treatment

“…The main reason is that sodium acetate is a low molecular organic acid salt, which is easy to be used by microorganisms. Denitrification deep bed filter completes the removal of SS and TN in the tank through filtration, carbon source dosing control and other mechanisms [7][8][9][10] .…”