Nitrogen amendment enhances the biological methanogenic potential of bituminous coal

Li, Yang; Qin, TianQi; Feng, Feisheng; Zhang, Yuanyuan; Xue, Sheng

doi:10.1016/j.fuel.2023.128932

Cited by 7 publications

(2 citation statements)

References 57 publications

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“…During the degradation of nitrogen-containing heterocyclic compounds, 2-oxoisocaproate dehydrogenase [EC:1.2.4.4], uric acid oxidase [EC:1.7.3.3], and dihydropyrimidine dehydrogenase [EC:1.3.1.2] act on the reductive cleavage, and dihydropyrimidinase [EC:3.5.2.2] acted on hydroxylation reactions to open nitrogen-containing heterocyclic compounds in the closed state, yielding organonitrogen compounds such as formiminoglycine, 3-ureidopropionate, and others . Meanwhile, this also indicates that the acquisition of nitrogen is an important process in microbial metabolism, and the low content of nitrogen in bituminous coal may limit microbial metabolism and growth . Shi et al found that microbial degradation of organic pollutants was related to nitrogen response and further determined that controlling organic nitrogen content could effectively influence the degradation of phenol, acetaminophen, and sulfamethoxazole in industrial wastewater.…”

Section: Discussionmentioning

confidence: 99%

“… 41 Meanwhile, this also indicates that the acquisition of nitrogen is an important process in microbial metabolism, and the low content of nitrogen in bituminous coal may limit microbial metabolism and growth. 42 Shi et al 43 found that microbial degradation of organic pollutants was related to nitrogen response and further determined that controlling organic nitrogen content could effectively influence the degradation of phenol, acetaminophen, and sulfamethoxazole in industrial wastewater. Wild et al 44 found that increasing nitrogen levels in the soil can promote soil microbial metabolic activity at low temperatures and further lead to the entry of soil carbon into the carbon cycle.…”

Section: Discussionmentioning

confidence: 99%

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Improved Formation of Biomethane by Enriched Microorganisms from Different Rank Coal Seams

Liu,

Zhou,

Xue

et al. 2024

ACS Omega

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The influence of enrichment of culturable microorganisms in in situ coal seams on biomethane production potential of other coal seams has been rarely studied. In this study, we enriched culturable microorganisms from three in situ coal seams with three coal ranks and conducted indoor anaerobic biomethane production experiments. Microbial community composition, gene functions, and metabolites in different culture units by 16S rRNA high-throughput sequencing combined with liquid chromatography-mass spectrometry-time-of-flight (LC-MS-TOF). The results showed that biomethane production in the bituminous coal group (BC)cc resulted in the highest methane yield of 243.3 μmol/g, which was 12.3 times higher than that in the control group (CK). Meanwhile, Methanosarcina was the dominant archaeal genus in the three experimental groups (37.42 ± 11.16−52.62 ± 2.10%), while its share in the CK was only 2.91 ± 0.48%. Based on the functional annotation, the relative abundance of functional genes in the three experimental groups was mainly related to the metabolism of nitrogen-containing heterocyclic compounds such as purines and pyrimidines. Metabolite analysis showed that enriched microorganisms promoted the degradation of a total of 778 organic substances in bituminous coal, including 55 significantly different metabolites (e.g., purines and pyrimidines). Based on genomic and metabolomic analyses, this paper reconstructed the heterocyclic compounds degradation coupled methane metabolism pathway and thereby preliminarily elucidated that enriched culturable bacteria from different coal-rank seams could promote the degradation of bituminous coal and intensify biogenic methane yields.

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Section: Discussionmentioning

confidence: 99%