Nitrogen and Oxygen Isotope Signatures of Nitrogen Compounds during Anammox in the Laboratory and a Wastewater Treatment Plant

Kotajima, Shotoku; Koba, Keisuke; Ikeda, Daisuke; Terada, Akihiko; Isaka, Kazuichi; Nishina, Kazuya; Kimura, Yuuya; Makabe, Akiko; Yano, M.; Fujitani, Hirotsugu; Ushiki, Norisuke; Tsuneda, Satoshi; Yoh, Muneoki

doi:10.1264/jsme2.me20031

Cited by 8 publications

(4 citation statements)

References 74 publications

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“…Jettenia , and Ca. Brocadia 10 , 19 , as well as in anammox incubation experiments 20 . This consistancy is also striking in light of the variation of 15 ε(NH 4 + ) observed in this study, and suggests that variations in substrate concentrations, reaction rates, or other physiological conditions are not strong controls on 15 ε(NO 2 – –N 2 ).…”

Section: Discussionmentioning

confidence: 98%

“…Such an inverse isotope effect appears to be a signature feature of microbial nitrite oxidation to nitrate, both under oxic and anoxic ( i.e. , anammox) conditions 58 , 59 In culture-based experiments with nitrite oxidizing bacteria (NOB), 15 ε(NO 2 – –NO 3 – ) has been found to vary between − 7.8‰ and − 23.6‰ 59,60 , while anammox bacteria have been shown to express 15 ε(NO 2 – –NO 3 – ) values in pure or highly-enriched cultures between − 30 and − 45‰ 10 , 19 , with values as low as − 78‰ in a wastewater incubation experiment 20 . In our experiments, we found N isotope effects that cover nearly this whole range (Fig.…”

Section: Discussionmentioning

confidence: 99%

“…, anoxic sediments and waters/ocean basins), its δ 15 N is thought to reflect the balance between N fixation, with a 15 ε near 0‰; aerobic ammonia oxidation, with a variable isotope effect between 14‰ and 46‰ 14 – 17 ; and assimilation (4‰ to 27‰) 18 . For anammox, a very limited number of isotope studies have constrained 15 N/ 14 N isotope effects in enriched cultures 10 , 19 , while one study has estimated their values in wastewater incubations 20 . These studies have established that there are significant variations amongst anammox microbial species with regards to the various N isotope effects associated with specific anammox reactions ( e.g.…”

Section: Introductionmentioning

confidence: 99%

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Nitrogen isotope effects can be used to diagnose N transformations in wastewater anammox systems

Magyar

Hausherr

Niederdorfer

et al. 2021

Sci Rep

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Anaerobic ammonium oxidation (anammox) plays an important role in aquatic systems as a sink of bioavailable nitrogen (N), and in engineered processes by removing ammonium from wastewater. The isotope effects anammox imparts in the N isotope signatures (15N/14N) of ammonium, nitrite, and nitrate can be used to estimate its role in environmental settings, to describe physiological and ecological variations in the anammox process, and possibly to optimize anammox-based wastewater treatment. We measured the stable N-isotope composition of ammonium, nitrite, and nitrate in wastewater cultivations of anammox bacteria. We find that the N isotope enrichment factor 15ε for the reduction of nitrite to N2 is consistent across all experimental conditions (13.5‰ ± 3.7‰), suggesting it reflects the composition of the anammox bacteria community. Values of 15ε for the oxidation of nitrite to nitrate (inverse isotope effect, − 16 to − 43‰) and for the reduction of ammonium to N2 (normal isotope effect, 19–32‰) are more variable, and likely controlled by experimental conditions. We argue that the variations in the isotope effects can be tied to the metabolism and physiology of anammox bacteria, and that the broad range of isotope effects observed for anammox introduces complications for analyzing N-isotope mass balances in natural systems.

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Section: Discussionmentioning

confidence: 98%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Nitrogen isotope effects can be used to diagnose N transformations in wastewater anammox systems

Magyar

Hausherr

Niederdorfer

et al. 2021

Sci Rep

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“…Isotopic fractionation [ 201 , 202 , 203 , 204 ] can cause the isotope amount ratio n( 15 N)/n( 14 N) to increase systematically through food chains via assimilation of N compounds in biomolecules such as proteins. Isotopic fractionation occurs because of assimilation, storage, and excretion of proteins and other N compounds.…”

Section: Tracking Nitrogen Uptake and Assimilation In Plantsmentioning

confidence: 99%

Multiple Facets of Nitrogen: From Atmospheric Gas to Indispensable Agricultural Input

Kabange

Lee

Shin

et al. 2022

Life

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Nitrogen (N) is a gas and the fifth most abundant element naturally found in the atmosphere. N’s role in agriculture and plant metabolism has been widely investigated for decades, and extensive information regarding this subject is available. However, the advent of sequencing technology and the advances in plant biotechnology, coupled with the growing interest in functional genomics-related studies and the various environmental challenges, have paved novel paths to rediscovering the fundamentals of N and its dynamics in physiological and biological processes, as well as biochemical reactions under both normal and stress conditions. This work provides a comprehensive review on multiple facets of N and N-containing compounds in plants disseminated in the literature to better appreciate N in its multiple dimensions. Here, some of the ancient but fundamental aspects of N are revived and the advances in our understanding of N in the metabolism of plants is portrayed. It is established that N is indispensable for achieving high plant productivity and fitness. However, the use of N-rich fertilizers in relatively higher amounts negatively affects the environment. Therefore, a paradigm shift is important to shape to the future use of N-rich fertilizers in crop production and their contribution to the current global greenhouse gases (GHGs) budget would help tackle current global environmental challenges toward a sustainable agriculture.

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Nitrous oxide emission mitigation from biological wastewater treatment – A review

Lee

Lin

Lei

2022

Bioresource Technology

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Nitrogen and Oxygen Isotope Signatures of Nitrogen Compounds during Anammox in the Laboratory and a Wastewater Treatment Plant

Cited by 8 publications

References 74 publications

Nitrogen isotope effects can be used to diagnose N transformations in wastewater anammox systems

Nitrogen isotope effects can be used to diagnose N transformations in wastewater anammox systems

Multiple Facets of Nitrogen: From Atmospheric Gas to Indispensable Agricultural Input

Nitrous oxide emission mitigation from biological wastewater treatment – A review

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