Nowadays, some relative warming temperatures related to climate change may be provided at the grafting time. Therefore, this study was conducted during two seasons (2018-2019) to study the effect of three callusing method (callusing room, callusing soil, callusing tunnel) and four grafting date (15 Jan., 1Feb., 15 Feb., 1 Mar.) for early (Flame seedless), medium (Thompson seedless) and late (Crimson seedless) grape varieties on grafted grape cuttings as short methods for transplant production. The results indicated that, the early grapes variety achieved higher grafting success on 1st Feb. grafting date as well as the late grape variety in callusing room and callusing soil methods. Also, Callusing soil achieved grafted success by 72.9%, 68.55% and 77.94% compared to callusing tunnel 37.3%, 45.9% and 55% for Flame seedless, Thompson seedless and Crimson seedless, respectively as mean of both seasons. High grafting success resulted from the high content of indole and sugars, along with low phenol content before callusing stage, as well as high indole and low sugars of grafts partner after callusing stage. while, higher phenols was accumulated in rootstock after callusing stage. There is no antagonistic effect between grafts partners. Callusing soil may be considered as an eco-friendly, sustainable and cheaper alternative tool for callusing of grafts cuttings.